Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01355

Experiment: H2 N-free with 10mM Urea

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01340 and MMP_RS01345 are separated by 7 nucleotidesMMP_RS01345 and MMP_RS01350 overlap by 1 nucleotidesMMP_RS01350 and MMP_RS01355 are separated by 54 nucleotidesMMP_RS01355 and MMP_RS01360 are separated by 29 nucleotidesMMP_RS01360 and MMP_RS01365 are separated by 98 nucleotides MMP_RS01340: MMP_RS01340 - KEOPS complex Pcc1-like subunit, at 250,204 to 250,455 _RS01340 MMP_RS01345: MMP_RS01345 - rRNA maturation protein, at 250,463 to 250,957 _RS01345 MMP_RS01350: MMP_RS01350 - DNA-directed RNA polymerase subunit P, at 250,957 to 251,097 _RS01350 MMP_RS01355: MMP_RS01355 - 50S ribosomal protein L37Ae, at 251,152 to 251,442 _RS01355 MMP_RS01360: MMP_RS01360 - ribosome assembly factor SBDS, at 251,472 to 252,176 _RS01360 MMP_RS01365: MMP_RS01365 - archaeal proteasome endopeptidase complex subunit alpha, at 252,275 to 253,054 _RS01365 Position (kb) 251 252Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 250.181 kb on + strandat 250.192 kb on + strandat 250.202 kb on - strandat 250.206 kb on + strandat 250.226 kb on - strandat 250.226 kb on - strandat 250.227 kb on + strandat 250.260 kb on + strandat 250.265 kb on - strandat 250.458 kb on - strandat 250.603 kb on + strandat 250.606 kb on - strandat 250.811 kb on - strandat 250.811 kb on - strandat 250.812 kb on + strandat 250.818 kb on + strandat 250.977 kb on - strandat 250.993 kb on - strandat 251.491 kb on + strandat 251.495 kb on - strandat 252.199 kb on + strandat 252.389 kb on - strand, within MMP_RS01365

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM Urea
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250,181 + -0.9
250,192 + -1.2
250,202 - +0.4
250,206 + +0.1
250,226 - +1.2
250,226 - +0.4
250,227 + +0.7
250,260 + -0.2
250,265 - -0.6
250,458 - -2.2
250,603 + +0.0
250,606 - -0.3
250,811 - +0.8
250,811 - -0.8
250,812 + +1.3
250,818 + -0.7
250,977 - +0.6
250,993 - +0.0
251,491 + -1.5
251,495 - +1.5
252,199 + +1.5
252,389 - MMP_RS01365 0.15 +4.5

Or see this region's nucleotide sequence