Experiment: H2 N-free with 10mM Urea
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00450 and MMP_RS00455 are separated by 194 nucleotides MMP_RS00455 and MMP_RS00460 are separated by 146 nucleotides MMP_RS00460 and MMP_RS00465 are separated by 27 nucleotides
MMP_RS00450: MMP_RS00450 - 4Fe-4S binding protein, at 88,705 to 89,550
_RS00450
MMP_RS00455: MMP_RS00455 - orotate phosphoribosyltransferase-like protein, at 89,745 to 90,362
_RS00455
MMP_RS00460: MMP_RS00460 - glutamine amidotransferase family protein, at 90,509 to 91,600
_RS00460
MMP_RS00465: MMP_RS00465 - alpha-hydroxy-acid oxidizing protein, at 91,628 to 93,160
_RS00465
Position (kb) 90
91
92 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 89.534 kb on + strand at 89.553 kb on - strand at 89.560 kb on - strand at 89.561 kb on + strand at 89.565 kb on - strand at 89.618 kb on - strand at 89.619 kb on + strand at 89.620 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.660 kb on - strand at 89.661 kb on + strand at 89.661 kb on + strand at 89.661 kb on + strand at 89.661 kb on + strand at 89.662 kb on - strand at 89.662 kb on - strand at 89.662 kb on - strand at 89.663 kb on + strand at 89.663 kb on + strand at 89.663 kb on + strand at 89.699 kb on - strand at 89.700 kb on + strand at 89.712 kb on - strand at 89.723 kb on - strand at 89.926 kb on - strand, within MMP_RS00455 at 89.969 kb on - strand, within MMP_RS00455 at 90.089 kb on - strand, within MMP_RS00455 at 90.089 kb on - strand, within MMP_RS00455 at 90.090 kb on + strand, within MMP_RS00455 at 90.091 kb on - strand, within MMP_RS00455 at 90.091 kb on - strand, within MMP_RS00455 at 90.091 kb on - strand, within MMP_RS00455 at 90.093 kb on - strand, within MMP_RS00455 at 90.093 kb on - strand, within MMP_RS00455 at 90.093 kb on - strand, within MMP_RS00455 at 90.094 kb on + strand, within MMP_RS00455 at 90.186 kb on + strand, within MMP_RS00455 at 90.319 kb on - strand at 90.478 kb on - strand at 91.203 kb on - strand at 91.703 kb on + strand at 91.888 kb on + strand at 91.953 kb on + strand at 92.001 kb on - strand at 92.002 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Urea remove 89,534 + +5.8 89,553 - +5.9 89,560 - +4.2 89,561 + +4.3 89,565 - +4.9 89,618 - +1.0 89,619 + +4.1 89,620 - +0.9 89,660 - -1.0 89,660 - +0.7 89,660 - +0.4 89,660 - -0.6 89,660 - +1.2 89,661 + +1.0 89,661 + +0.2 89,661 + -0.8 89,661 + -0.3 89,662 - +0.5 89,662 - -0.7 89,662 - -0.7 89,663 + +1.1 89,663 + -0.2 89,663 + -0.3 89,699 - +0.7 89,700 + +1.5 89,712 - +2.2 89,723 - +1.5 89,926 - MMP_RS00455 0.29 +2.3 89,969 - MMP_RS00455 0.36 +2.0 90,089 - MMP_RS00455 0.56 +0.8 90,089 - MMP_RS00455 0.56 +1.6 90,090 + MMP_RS00455 0.56 -1.2 90,091 - MMP_RS00455 0.56 +1.1 90,091 - MMP_RS00455 0.56 -0.8 90,091 - MMP_RS00455 0.56 +0.7 90,093 - MMP_RS00455 0.56 +0.1 90,093 - MMP_RS00455 0.56 +1.9 90,093 - MMP_RS00455 0.56 +1.7 90,094 + MMP_RS00455 0.56 +2.3 90,186 + MMP_RS00455 0.71 +0.6 90,319 - +2.1 90,478 - +1.5 91,203 - +2.6 91,703 + +0.9 91,888 + +1.9 91,953 + +3.2 92,001 - +0.5 92,002 + +2.4
Or see this region's nucleotide sequence