Experiment: H2 N-free with 10mM B-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03610 and MMP_RS03615 are separated by 66 nucleotides MMP_RS03615 and MMP_RS03620 are separated by 5 nucleotides MMP_RS03620 and MMP_RS03625 are separated by 85 nucleotides
MMP_RS03610: MMP_RS03610 - NCS2 family permease, at 677,464 to 678,765
_RS03610
MMP_RS03615: MMP_RS03615 - 4Fe-4S binding protein, at 678,832 to 679,533
_RS03615
MMP_RS03620: MMP_RS03620 - hypothetical protein, at 679,539 to 680,084
_RS03620
MMP_RS03625: MMP_RS03625 - cell wall-binding protein, at 680,170 to 681,129
_RS03625
Position (kb) 679
680
681 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 678.723 kb on + strand at 678.801 kb on - strand at 678.892 kb on + strand at 678.892 kb on + strand at 678.972 kb on - strand, within MMP_RS03615 at 679.044 kb on + strand, within MMP_RS03615 at 679.099 kb on - strand, within MMP_RS03615 at 679.101 kb on - strand, within MMP_RS03615 at 679.102 kb on + strand, within MMP_RS03615 at 679.166 kb on - strand, within MMP_RS03615 at 679.167 kb on + strand, within MMP_RS03615 at 679.174 kb on + strand, within MMP_RS03615 at 679.179 kb on - strand, within MMP_RS03615 at 679.215 kb on + strand, within MMP_RS03615 at 679.252 kb on - strand, within MMP_RS03615 at 679.330 kb on - strand, within MMP_RS03615 at 679.332 kb on - strand, within MMP_RS03615 at 679.384 kb on - strand, within MMP_RS03615 at 679.385 kb on + strand, within MMP_RS03615 at 679.461 kb on - strand, within MMP_RS03615 at 679.484 kb on + strand at 679.668 kb on - strand, within MMP_RS03620 at 679.669 kb on + strand, within MMP_RS03620 at 679.680 kb on - strand, within MMP_RS03620 at 679.702 kb on - strand, within MMP_RS03620 at 679.704 kb on - strand, within MMP_RS03620 at 679.705 kb on + strand, within MMP_RS03620 at 679.748 kb on - strand, within MMP_RS03620 at 679.749 kb on + strand, within MMP_RS03620 at 679.753 kb on + strand, within MMP_RS03620 at 679.811 kb on - strand, within MMP_RS03620 at 679.814 kb on + strand, within MMP_RS03620 at 679.847 kb on + strand, within MMP_RS03620 at 679.902 kb on - strand, within MMP_RS03620 at 679.960 kb on - strand, within MMP_RS03620 at 680.096 kb on - strand at 680.097 kb on + strand at 680.099 kb on + strand at 680.099 kb on + strand at 680.156 kb on + strand at 680.158 kb on + strand at 680.158 kb on + strand at 680.242 kb on - strand at 680.388 kb on + strand, within MMP_RS03625 at 680.393 kb on - strand, within MMP_RS03625 at 680.393 kb on - strand, within MMP_RS03625 at 680.394 kb on + strand, within MMP_RS03625 at 680.414 kb on - strand, within MMP_RS03625 at 680.603 kb on - strand, within MMP_RS03625 at 680.609 kb on - strand, within MMP_RS03625 at 680.624 kb on + strand, within MMP_RS03625 at 680.947 kb on - strand, within MMP_RS03625 at 681.015 kb on - strand, within MMP_RS03625 at 681.015 kb on - strand, within MMP_RS03625
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM B-Alanine remove 678,723 + -3.1 678,801 - -0.8 678,892 + +1.6 678,892 + +1.0 678,972 - MMP_RS03615 0.20 -0.2 679,044 + MMP_RS03615 0.30 +0.5 679,099 - MMP_RS03615 0.38 -0.2 679,101 - MMP_RS03615 0.38 -0.4 679,102 + MMP_RS03615 0.38 +0.7 679,166 - MMP_RS03615 0.48 -0.3 679,167 + MMP_RS03615 0.48 +0.4 679,174 + MMP_RS03615 0.49 -0.1 679,179 - MMP_RS03615 0.49 -0.3 679,215 + MMP_RS03615 0.55 +0.5 679,252 - MMP_RS03615 0.60 +0.6 679,330 - MMP_RS03615 0.71 -1.4 679,332 - MMP_RS03615 0.71 +1.2 679,384 - MMP_RS03615 0.79 -0.2 679,385 + MMP_RS03615 0.79 -1.2 679,461 - MMP_RS03615 0.90 +1.2 679,484 + +1.7 679,668 - MMP_RS03620 0.24 +0.7 679,669 + MMP_RS03620 0.24 -2.1 679,680 - MMP_RS03620 0.26 -1.6 679,702 - MMP_RS03620 0.30 +0.4 679,704 - MMP_RS03620 0.30 +0.1 679,705 + MMP_RS03620 0.30 +0.2 679,748 - MMP_RS03620 0.38 -0.2 679,749 + MMP_RS03620 0.38 -1.5 679,753 + MMP_RS03620 0.39 -0.2 679,811 - MMP_RS03620 0.50 -1.3 679,814 + MMP_RS03620 0.50 -0.2 679,847 + MMP_RS03620 0.56 -0.1 679,902 - MMP_RS03620 0.66 -1.4 679,960 - MMP_RS03620 0.77 -0.2 680,096 - -0.5 680,097 + +2.6 680,099 + +0.7 680,099 + +0.1 680,156 + -0.6 680,158 + -0.3 680,158 + -0.2 680,242 - -0.6 680,388 + MMP_RS03625 0.23 +0.7 680,393 - MMP_RS03625 0.23 -1.0 680,393 - MMP_RS03625 0.23 +0.0 680,394 + MMP_RS03625 0.23 +0.6 680,414 - MMP_RS03625 0.25 -2.5 680,603 - MMP_RS03625 0.45 -0.4 680,609 - MMP_RS03625 0.46 -0.0 680,624 + MMP_RS03625 0.47 +0.7 680,947 - MMP_RS03625 0.81 -0.2 681,015 - MMP_RS03625 0.88 +0.1 681,015 - MMP_RS03625 0.88 -1.0
Or see this region's nucleotide sequence