Experiment: H2 N-free with 10mM B-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03560 and MMP_RS03565 are separated by 90 nucleotides MMP_RS03565 and MMP_RS03570 are separated by 36 nucleotides MMP_RS03570 and MMP_RS03575 are separated by 164 nucleotides MMP_RS03575 and MMP_RS03580 are separated by 99 nucleotides
MMP_RS03560: MMP_RS03560 - Na+/H+ antiporter NhaC family protein, at 666,725 to 668,302
_RS03560
MMP_RS03565: MMP_RS03565 - uracil phosphoribosyltransferase, at 668,393 to 669,088
_RS03565
MMP_RS03570: MMP_RS03570 - uracil-xanthine permease family protein, at 669,125 to 670,366
_RS03570
MMP_RS03575: MMP_RS03575 - ECF transporter S component, at 670,531 to 671,097
_RS03575
MMP_RS03580: MMP_RS03580 - signal recognition particle-docking protein FtsY, at 671,197 to 672,348
_RS03580
Position (kb)
669
670
671 Strain fitness (log2 ratio)
-2
-1
0
1 at 668.144 kb on + strand, within MMP_RS03560 at 668.147 kb on - strand at 668.152 kb on - strand at 668.173 kb on - strand at 668.193 kb on + strand at 668.193 kb on + strand at 668.229 kb on - strand at 668.285 kb on - strand at 668.286 kb on + strand at 668.289 kb on + strand at 668.302 kb on - strand at 668.302 kb on - strand at 668.307 kb on + strand at 668.307 kb on + strand at 668.308 kb on - strand at 668.309 kb on + strand at 668.329 kb on + strand at 668.354 kb on + strand at 668.354 kb on + strand at 668.356 kb on + strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.386 kb on - strand at 668.480 kb on - strand, within MMP_RS03565 at 668.480 kb on - strand, within MMP_RS03565 at 668.484 kb on - strand, within MMP_RS03565 at 668.494 kb on - strand, within MMP_RS03565 at 668.591 kb on - strand, within MMP_RS03565 at 668.648 kb on + strand, within MMP_RS03565 at 668.719 kb on + strand, within MMP_RS03565 at 668.729 kb on - strand, within MMP_RS03565 at 668.730 kb on + strand, within MMP_RS03565 at 668.734 kb on - strand, within MMP_RS03565 at 668.842 kb on - strand, within MMP_RS03565 at 668.845 kb on - strand, within MMP_RS03565 at 668.860 kb on - strand, within MMP_RS03565 at 669.032 kb on - strand at 669.084 kb on + strand at 669.088 kb on - strand at 669.114 kb on + strand at 669.119 kb on + strand at 669.279 kb on + strand, within MMP_RS03570 at 669.310 kb on - strand, within MMP_RS03570 at 669.364 kb on - strand, within MMP_RS03570 at 669.416 kb on + strand, within MMP_RS03570 at 669.443 kb on - strand, within MMP_RS03570 at 669.687 kb on + strand, within MMP_RS03570 at 669.798 kb on + strand, within MMP_RS03570 at 669.825 kb on + strand, within MMP_RS03570 at 669.867 kb on + strand, within MMP_RS03570 at 669.883 kb on - strand, within MMP_RS03570 at 669.884 kb on + strand, within MMP_RS03570 at 669.906 kb on + strand, within MMP_RS03570 at 669.914 kb on - strand, within MMP_RS03570 at 669.931 kb on - strand, within MMP_RS03570 at 669.945 kb on - strand, within MMP_RS03570 at 670.069 kb on - strand, within MMP_RS03570 at 670.334 kb on - strand at 670.335 kb on + strand at 670.350 kb on + strand at 670.350 kb on + strand at 670.350 kb on + strand at 670.356 kb on + strand at 670.392 kb on + strand at 670.437 kb on + strand at 670.440 kb on - strand at 670.466 kb on - strand at 670.536 kb on - strand at 670.558 kb on - strand at 670.559 kb on + strand at 670.583 kb on + strand at 670.598 kb on - strand, within MMP_RS03575 at 670.616 kb on + strand, within MMP_RS03575 at 670.655 kb on - strand, within MMP_RS03575 at 670.656 kb on + strand, within MMP_RS03575 at 670.656 kb on + strand, within MMP_RS03575 at 670.656 kb on + strand, within MMP_RS03575 at 670.671 kb on - strand, within MMP_RS03575 at 670.683 kb on - strand, within MMP_RS03575 at 670.778 kb on + strand, within MMP_RS03575 at 670.805 kb on + strand, within MMP_RS03575 at 670.874 kb on + strand, within MMP_RS03575 at 670.908 kb on - strand, within MMP_RS03575 at 670.908 kb on - strand, within MMP_RS03575 at 670.908 kb on - strand, within MMP_RS03575 at 670.981 kb on - strand, within MMP_RS03575 at 671.057 kb on + strand at 671.088 kb on - strand at 671.089 kb on + strand at 671.143 kb on + strand at 671.143 kb on + strand at 671.144 kb on - strand at 671.144 kb on - strand at 671.152 kb on + strand at 671.185 kb on - strand at 671.312 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM B-Alanine remove 668,144 + MMP_RS03560 0.90 -1.3 668,147 - +0.5 668,152 - +0.7 668,173 - +0.2 668,193 + +0.3 668,193 + +0.6 668,229 - -1.9 668,285 - -1.4 668,286 + -0.8 668,289 + -0.5 668,302 - +0.9 668,302 - +0.8 668,307 + -0.1 668,307 + +0.3 668,308 - +0.2 668,309 + -1.6 668,329 + -0.9 668,354 + +0.2 668,354 + +0.3 668,356 + -0.3 668,386 - +0.7 668,386 - +1.0 668,386 - -0.8 668,386 - +1.1 668,386 - -0.4 668,480 - MMP_RS03565 0.12 +0.1 668,480 - MMP_RS03565 0.12 +0.9 668,484 - MMP_RS03565 0.13 -0.3 668,494 - MMP_RS03565 0.15 +0.0 668,591 - MMP_RS03565 0.28 -0.2 668,648 + MMP_RS03565 0.37 -1.2 668,719 + MMP_RS03565 0.47 -1.3 668,729 - MMP_RS03565 0.48 +1.1 668,730 + MMP_RS03565 0.48 -0.7 668,734 - MMP_RS03565 0.49 +0.2 668,842 - MMP_RS03565 0.65 -0.2 668,845 - MMP_RS03565 0.65 -1.2 668,860 - MMP_RS03565 0.67 -0.1 669,032 - +0.2 669,084 + -0.2 669,088 - +0.0 669,114 + -1.6 669,119 + +0.4 669,279 + MMP_RS03570 0.12 -0.9 669,310 - MMP_RS03570 0.15 -2.3 669,364 - MMP_RS03570 0.19 -0.3 669,416 + MMP_RS03570 0.23 +0.1 669,443 - MMP_RS03570 0.26 -0.7 669,687 + MMP_RS03570 0.45 -0.4 669,798 + MMP_RS03570 0.54 -0.6 669,825 + MMP_RS03570 0.56 +0.4 669,867 + MMP_RS03570 0.60 +0.3 669,883 - MMP_RS03570 0.61 +0.2 669,884 + MMP_RS03570 0.61 -0.1 669,906 + MMP_RS03570 0.63 +0.4 669,914 - MMP_RS03570 0.64 -1.1 669,931 - MMP_RS03570 0.65 +0.2 669,945 - MMP_RS03570 0.66 -1.1 670,069 - MMP_RS03570 0.76 -0.3 670,334 - -0.6 670,335 + +0.6 670,350 + +1.0 670,350 + +0.4 670,350 + +1.0 670,356 + -0.0 670,392 + +0.1 670,437 + +1.0 670,440 - +0.1 670,466 - -0.7 670,536 - -0.3 670,558 - +1.0 670,559 + -0.4 670,583 + -0.0 670,598 - MMP_RS03575 0.12 +0.4 670,616 + MMP_RS03575 0.15 +0.3 670,655 - MMP_RS03575 0.22 -0.6 670,656 + MMP_RS03575 0.22 -1.0 670,656 + MMP_RS03575 0.22 +0.6 670,656 + MMP_RS03575 0.22 -0.1 670,671 - MMP_RS03575 0.25 +0.5 670,683 - MMP_RS03575 0.27 -0.7 670,778 + MMP_RS03575 0.44 -1.3 670,805 + MMP_RS03575 0.48 +0.8 670,874 + MMP_RS03575 0.60 -0.4 670,908 - MMP_RS03575 0.66 +1.2 670,908 - MMP_RS03575 0.66 +0.6 670,908 - MMP_RS03575 0.66 -0.1 670,981 - MMP_RS03575 0.79 +0.5 671,057 + -0.5 671,088 - +0.8 671,089 + -0.4 671,143 + +1.0 671,143 + -1.2 671,144 - -0.6 671,144 - -0.4 671,152 + +0.2 671,185 - +0.4 671,312 + +0.4
Or see this region's nucleotide sequence