Experiment: H2 N-free with 10mM B-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02620 and MMP_RS02625 overlap by 7 nucleotides MMP_RS02625 and MMP_RS02630 are separated by 250 nucleotides
MMP_RS02620: MMP_RS02620 - DEAD/DEAH box helicase, at 496,024 to 498,303
_RS02620
MMP_RS02625: MMP_RS02625 - ribonuclease H-like domain-containing protein, at 498,297 to 499,391
_RS02625
MMP_RS02630: MMP_RS02630 - rubredoxin, at 499,642 to 501,126
_RS02630
Position (kb) 498
499
500 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 497.411 kb on + strand, within MMP_RS02620 at 497.511 kb on - strand, within MMP_RS02620 at 497.512 kb on + strand, within MMP_RS02620 at 497.584 kb on - strand, within MMP_RS02620 at 497.633 kb on + strand, within MMP_RS02620 at 497.654 kb on - strand, within MMP_RS02620 at 497.683 kb on - strand, within MMP_RS02620 at 497.697 kb on - strand, within MMP_RS02620 at 497.720 kb on - strand, within MMP_RS02620 at 497.720 kb on - strand, within MMP_RS02620 at 497.721 kb on + strand, within MMP_RS02620 at 497.741 kb on + strand, within MMP_RS02620 at 497.792 kb on + strand, within MMP_RS02620 at 497.863 kb on + strand, within MMP_RS02620 at 497.883 kb on + strand, within MMP_RS02620 at 498.089 kb on + strand at 498.119 kb on - strand at 498.167 kb on + strand at 498.231 kb on - strand at 498.320 kb on - strand at 498.339 kb on - strand at 498.348 kb on - strand at 498.348 kb on - strand at 498.348 kb on - strand at 498.363 kb on - strand at 498.403 kb on - strand at 498.404 kb on + strand at 498.465 kb on - strand, within MMP_RS02625 at 498.480 kb on + strand, within MMP_RS02625 at 498.580 kb on - strand, within MMP_RS02625 at 498.603 kb on + strand, within MMP_RS02625 at 498.612 kb on + strand, within MMP_RS02625 at 498.810 kb on - strand, within MMP_RS02625 at 498.822 kb on - strand, within MMP_RS02625 at 498.884 kb on - strand, within MMP_RS02625 at 499.068 kb on - strand, within MMP_RS02625 at 499.124 kb on + strand, within MMP_RS02625 at 499.165 kb on - strand, within MMP_RS02625 at 499.173 kb on - strand, within MMP_RS02625 at 499.371 kb on - strand at 499.412 kb on - strand at 499.413 kb on + strand at 499.444 kb on - strand at 499.475 kb on + strand at 499.513 kb on + strand at 499.561 kb on - strand at 499.562 kb on + strand at 499.563 kb on - strand at 499.563 kb on - strand at 499.564 kb on + strand at 499.567 kb on - strand at 499.574 kb on - strand at 499.575 kb on + strand at 499.578 kb on + strand at 499.589 kb on + strand at 500.097 kb on - strand, within MMP_RS02630 at 500.289 kb on - strand, within MMP_RS02630 at 500.289 kb on - strand, within MMP_RS02630 at 500.294 kb on + strand, within MMP_RS02630 at 500.300 kb on - strand, within MMP_RS02630 at 500.305 kb on - strand, within MMP_RS02630 at 500.305 kb on - strand, within MMP_RS02630 at 500.306 kb on + strand, within MMP_RS02630 at 500.310 kb on + strand, within MMP_RS02630
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM B-Alanine remove 497,411 + MMP_RS02620 0.61 +0.4 497,511 - MMP_RS02620 0.65 -0.0 497,512 + MMP_RS02620 0.65 -0.4 497,584 - MMP_RS02620 0.68 -1.3 497,633 + MMP_RS02620 0.71 -0.1 497,654 - MMP_RS02620 0.71 -0.4 497,683 - MMP_RS02620 0.73 +0.1 497,697 - MMP_RS02620 0.73 +1.3 497,720 - MMP_RS02620 0.74 +0.2 497,720 - MMP_RS02620 0.74 -0.9 497,721 + MMP_RS02620 0.74 -0.5 497,741 + MMP_RS02620 0.75 -0.4 497,792 + MMP_RS02620 0.78 -2.3 497,863 + MMP_RS02620 0.81 +0.3 497,883 + MMP_RS02620 0.82 -1.6 498,089 + -0.3 498,119 - -1.3 498,167 + -0.9 498,231 - +0.8 498,320 - +1.1 498,339 - -1.2 498,348 - -0.3 498,348 - +1.5 498,348 - -0.6 498,363 - -0.2 498,403 - +0.5 498,404 + +0.1 498,465 - MMP_RS02625 0.15 +1.0 498,480 + MMP_RS02625 0.17 +0.2 498,580 - MMP_RS02625 0.26 -1.2 498,603 + MMP_RS02625 0.28 -1.0 498,612 + MMP_RS02625 0.29 -1.0 498,810 - MMP_RS02625 0.47 -3.1 498,822 - MMP_RS02625 0.48 -0.0 498,884 - MMP_RS02625 0.54 -0.2 499,068 - MMP_RS02625 0.70 -0.1 499,124 + MMP_RS02625 0.76 -1.2 499,165 - MMP_RS02625 0.79 +0.4 499,173 - MMP_RS02625 0.80 +0.2 499,371 - +0.2 499,412 - +0.3 499,413 + -0.1 499,444 - -0.2 499,475 + -0.7 499,513 + -0.3 499,561 - -1.1 499,562 + -1.2 499,563 - +0.6 499,563 - -0.7 499,564 + +0.4 499,567 - -1.9 499,574 - -1.0 499,575 + +1.0 499,578 + -0.0 499,589 + +1.1 500,097 - MMP_RS02630 0.31 -0.3 500,289 - MMP_RS02630 0.44 -0.5 500,289 - MMP_RS02630 0.44 +0.8 500,294 + MMP_RS02630 0.44 -0.4 500,300 - MMP_RS02630 0.44 -0.4 500,305 - MMP_RS02630 0.45 -0.2 500,305 - MMP_RS02630 0.45 -0.0 500,306 + MMP_RS02630 0.45 -0.1 500,310 + MMP_RS02630 0.45 -1.0
Or see this region's nucleotide sequence