Experiment: H2 N-free with 10mM B-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02580 and MMP_RS02585 are separated by 230 nucleotides MMP_RS02585 and MMP_RS02590 are separated by 86 nucleotides MMP_RS02590 and MMP_RS02595 are separated by 212 nucleotides
MMP_RS02580: MMP_RS02580 - cupin domain-containing protein, at 482,858 to 483,295
_RS02580
MMP_RS02585: MMP_RS02585 - methyl-accepting chemotaxis protein, at 483,526 to 485,715
_RS02585
MMP_RS02590: MMP_RS02590 - low molecular weight phosphatase family protein, at 485,802 to 486,212
_RS02590
MMP_RS02595: MMP_RS02595 - putative zinc-binding protein, at 486,425 to 486,886
_RS02595
Position (kb) 483
484
485
486 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 482.544 kb on - strand at 482.677 kb on - strand at 482.756 kb on - strand at 482.756 kb on - strand at 482.756 kb on - strand at 482.757 kb on + strand at 482.757 kb on + strand at 482.757 kb on + strand at 482.757 kb on + strand at 482.757 kb on + strand at 482.758 kb on - strand at 482.758 kb on - strand at 482.758 kb on - strand at 482.759 kb on + strand at 482.760 kb on - strand at 482.760 kb on - strand at 482.760 kb on - strand at 482.760 kb on - strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.761 kb on + strand at 482.762 kb on - strand at 482.762 kb on - strand at 482.762 kb on - strand at 482.824 kb on + strand at 482.864 kb on - strand at 482.896 kb on - strand at 482.897 kb on + strand at 482.897 kb on + strand at 482.897 kb on + strand at 482.897 kb on + strand at 482.936 kb on - strand, within MMP_RS02580 at 482.937 kb on + strand, within MMP_RS02580 at 482.959 kb on + strand, within MMP_RS02580 at 483.026 kb on + strand, within MMP_RS02580 at 483.031 kb on - strand, within MMP_RS02580 at 483.032 kb on + strand, within MMP_RS02580 at 483.152 kb on - strand, within MMP_RS02580 at 483.168 kb on - strand, within MMP_RS02580 at 483.169 kb on + strand, within MMP_RS02580 at 483.279 kb on + strand at 483.284 kb on + strand at 483.297 kb on - strand at 483.320 kb on - strand at 483.358 kb on - strand at 483.359 kb on + strand at 483.361 kb on + strand at 483.363 kb on - strand at 483.364 kb on + strand at 483.413 kb on - strand at 483.436 kb on - strand at 483.436 kb on - strand at 483.436 kb on - strand at 483.437 kb on + strand at 483.450 kb on + strand at 483.451 kb on - strand at 483.451 kb on - strand at 483.499 kb on - strand at 483.500 kb on + strand at 483.500 kb on + strand at 483.503 kb on + strand at 483.514 kb on - strand at 483.515 kb on + strand at 483.515 kb on + strand at 483.523 kb on - strand at 483.523 kb on - strand at 483.524 kb on + strand at 483.534 kb on + strand at 483.560 kb on - strand at 483.566 kb on - strand at 483.570 kb on - strand at 483.571 kb on + strand at 483.749 kb on - strand, within MMP_RS02585 at 483.749 kb on - strand, within MMP_RS02585 at 483.754 kb on + strand, within MMP_RS02585 at 483.849 kb on - strand, within MMP_RS02585 at 483.849 kb on - strand, within MMP_RS02585 at 483.867 kb on - strand, within MMP_RS02585 at 483.867 kb on - strand, within MMP_RS02585 at 483.868 kb on + strand, within MMP_RS02585 at 483.868 kb on + strand, within MMP_RS02585 at 483.868 kb on + strand, within MMP_RS02585 at 483.868 kb on + strand, within MMP_RS02585 at 483.871 kb on + strand, within MMP_RS02585 at 483.871 kb on + strand, within MMP_RS02585 at 483.871 kb on + strand, within MMP_RS02585 at 483.998 kb on - strand, within MMP_RS02585 at 484.002 kb on - strand, within MMP_RS02585 at 484.064 kb on - strand, within MMP_RS02585 at 484.239 kb on - strand, within MMP_RS02585 at 484.239 kb on - strand, within MMP_RS02585 at 484.241 kb on - strand, within MMP_RS02585 at 484.242 kb on + strand, within MMP_RS02585 at 484.262 kb on + strand, within MMP_RS02585 at 484.296 kb on - strand, within MMP_RS02585 at 484.337 kb on - strand, within MMP_RS02585 at 484.344 kb on - strand, within MMP_RS02585 at 484.347 kb on - strand, within MMP_RS02585 at 484.349 kb on - strand, within MMP_RS02585 at 484.386 kb on - strand, within MMP_RS02585 at 484.416 kb on + strand, within MMP_RS02585 at 484.459 kb on - strand, within MMP_RS02585 at 484.460 kb on + strand, within MMP_RS02585 at 484.460 kb on + strand, within MMP_RS02585 at 484.460 kb on + strand, within MMP_RS02585 at 484.532 kb on + strand, within MMP_RS02585 at 484.790 kb on - strand, within MMP_RS02585 at 484.791 kb on + strand, within MMP_RS02585 at 484.827 kb on - strand, within MMP_RS02585 at 484.972 kb on - strand, within MMP_RS02585 at 485.327 kb on - strand, within MMP_RS02585 at 485.335 kb on + strand, within MMP_RS02585 at 485.335 kb on + strand, within MMP_RS02585 at 485.685 kb on + strand at 485.689 kb on + strand at 485.754 kb on + strand at 485.783 kb on - strand at 485.844 kb on - strand, within MMP_RS02590 at 485.898 kb on - strand, within MMP_RS02590 at 485.898 kb on - strand, within MMP_RS02590 at 485.898 kb on - strand, within MMP_RS02590 at 485.898 kb on - strand, within MMP_RS02590 at 485.898 kb on - strand, within MMP_RS02590 at 485.898 kb on - strand, within MMP_RS02590 at 485.899 kb on + strand, within MMP_RS02590 at 485.965 kb on + strand, within MMP_RS02590 at 485.965 kb on + strand, within MMP_RS02590 at 485.993 kb on - strand, within MMP_RS02590 at 486.169 kb on + strand, within MMP_RS02590 at 486.207 kb on + strand at 486.232 kb on - strand at 486.244 kb on - strand at 486.249 kb on - strand at 486.249 kb on - strand at 486.253 kb on - strand at 486.253 kb on - strand at 486.253 kb on - strand at 486.254 kb on + strand at 486.254 kb on + strand at 486.255 kb on - strand at 486.255 kb on - strand at 486.256 kb on + strand at 486.269 kb on - strand at 486.269 kb on - strand at 486.270 kb on + strand at 486.271 kb on - strand at 486.271 kb on - strand at 486.272 kb on + strand at 486.281 kb on - strand at 486.291 kb on - strand at 486.292 kb on + strand at 486.293 kb on - strand at 486.319 kb on - strand at 486.346 kb on - strand at 486.347 kb on + strand at 486.712 kb on - strand, within MMP_RS02595
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM B-Alanine remove 482,544 - -0.0 482,677 - +1.4 482,756 - +0.2 482,756 - +1.0 482,756 - -0.3 482,757 + -0.1 482,757 + -0.2 482,757 + +0.5 482,757 + +0.3 482,757 + +0.1 482,758 - +1.1 482,758 - -0.0 482,758 - -1.4 482,759 + +0.5 482,760 - -0.0 482,760 - +0.0 482,760 - +0.1 482,760 - +0.3 482,761 + +1.3 482,761 + +0.9 482,761 + -0.1 482,761 + -0.5 482,761 + +0.2 482,761 + +0.3 482,761 + +0.6 482,761 + +1.1 482,762 - -0.0 482,762 - -1.2 482,762 - +1.5 482,824 + +0.5 482,864 - -0.9 482,896 - +0.7 482,897 + +0.9 482,897 + -0.5 482,897 + +0.7 482,897 + +1.4 482,936 - MMP_RS02580 0.18 -0.8 482,937 + MMP_RS02580 0.18 -2.6 482,959 + MMP_RS02580 0.23 +0.3 483,026 + MMP_RS02580 0.38 +0.0 483,031 - MMP_RS02580 0.39 +0.0 483,032 + MMP_RS02580 0.40 +1.2 483,152 - MMP_RS02580 0.67 -0.3 483,168 - MMP_RS02580 0.71 +0.5 483,169 + MMP_RS02580 0.71 +0.3 483,279 + -0.8 483,284 + +0.8 483,297 - +0.7 483,320 - +1.1 483,358 - -0.1 483,359 + -0.2 483,361 + -1.0 483,363 - -0.2 483,364 + -0.3 483,413 - -0.2 483,436 - -1.2 483,436 - +0.6 483,436 - -0.3 483,437 + +0.0 483,450 + +1.2 483,451 - -0.2 483,451 - -1.2 483,499 - -0.6 483,500 + -0.5 483,500 + -0.7 483,503 + +0.2 483,514 - -0.6 483,515 + +3.4 483,515 + -0.6 483,523 - +0.5 483,523 - +0.3 483,524 + -0.1 483,534 + +0.2 483,560 - +0.6 483,566 - +1.2 483,570 - -0.4 483,571 + -0.7 483,749 - MMP_RS02585 0.10 -0.5 483,749 - MMP_RS02585 0.10 -0.2 483,754 + MMP_RS02585 0.10 -0.5 483,849 - MMP_RS02585 0.15 -1.1 483,849 - MMP_RS02585 0.15 +0.7 483,867 - MMP_RS02585 0.16 -0.4 483,867 - MMP_RS02585 0.16 +0.0 483,868 + MMP_RS02585 0.16 +1.5 483,868 + MMP_RS02585 0.16 -0.0 483,868 + MMP_RS02585 0.16 -0.4 483,868 + MMP_RS02585 0.16 -0.9 483,871 + MMP_RS02585 0.16 +0.0 483,871 + MMP_RS02585 0.16 -1.9 483,871 + MMP_RS02585 0.16 -0.5 483,998 - MMP_RS02585 0.22 -0.8 484,002 - MMP_RS02585 0.22 +0.2 484,064 - MMP_RS02585 0.25 +0.7 484,239 - MMP_RS02585 0.33 +0.2 484,239 - MMP_RS02585 0.33 -0.5 484,241 - MMP_RS02585 0.33 -0.6 484,242 + MMP_RS02585 0.33 -0.7 484,262 + MMP_RS02585 0.34 +0.0 484,296 - MMP_RS02585 0.35 -0.4 484,337 - MMP_RS02585 0.37 +0.3 484,344 - MMP_RS02585 0.37 +0.2 484,347 - MMP_RS02585 0.37 -1.8 484,349 - MMP_RS02585 0.38 -0.2 484,386 - MMP_RS02585 0.39 +0.0 484,416 + MMP_RS02585 0.41 -0.7 484,459 - MMP_RS02585 0.43 +0.0 484,460 + MMP_RS02585 0.43 -0.9 484,460 + MMP_RS02585 0.43 -0.7 484,460 + MMP_RS02585 0.43 +0.6 484,532 + MMP_RS02585 0.46 -0.2 484,790 - MMP_RS02585 0.58 +0.4 484,791 + MMP_RS02585 0.58 +0.6 484,827 - MMP_RS02585 0.59 -0.1 484,972 - MMP_RS02585 0.66 -0.9 485,327 - MMP_RS02585 0.82 +0.7 485,335 + MMP_RS02585 0.83 +0.2 485,335 + MMP_RS02585 0.83 -0.5 485,685 + +5.7 485,689 + -0.2 485,754 + +0.6 485,783 - -0.6 485,844 - MMP_RS02590 0.10 +0.4 485,898 - MMP_RS02590 0.23 +0.3 485,898 - MMP_RS02590 0.23 +1.4 485,898 - MMP_RS02590 0.23 -0.1 485,898 - MMP_RS02590 0.23 +0.7 485,898 - MMP_RS02590 0.23 -0.2 485,898 - MMP_RS02590 0.23 +0.2 485,899 + MMP_RS02590 0.24 -0.1 485,965 + MMP_RS02590 0.40 -0.4 485,965 + MMP_RS02590 0.40 -2.6 485,993 - MMP_RS02590 0.46 +0.6 486,169 + MMP_RS02590 0.89 +0.5 486,207 + -0.4 486,232 - +0.5 486,244 - +0.1 486,249 - +0.2 486,249 - -0.3 486,253 - -0.1 486,253 - -0.1 486,253 - +0.2 486,254 + -0.9 486,254 + +0.1 486,255 - -0.3 486,255 - +1.4 486,256 + -1.8 486,269 - -1.6 486,269 - -0.4 486,270 + -1.0 486,271 - +0.2 486,271 - -0.3 486,272 + -0.6 486,281 - -0.3 486,291 - -0.0 486,292 + -1.1 486,293 - -0.9 486,319 - +0.1 486,346 - +1.6 486,347 + -0.9 486,712 - MMP_RS02595 0.62 -0.0
Or see this region's nucleotide sequence