Experiment: H2 N-free with 10mM B-Alanine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00855 and MMP_RS00860 are separated by 4 nucleotides MMP_RS00860 and MMP_RS00865 are separated by 381 nucleotides MMP_RS00865 and MMP_RS08930 are separated by 268 nucleotides MMP_RS08930 and MMP_RS00870 are separated by 25 nucleotides
MMP_RS00855: MMP_RS00855 - DUF367 family protein, at 161,087 to 161,602
_RS00855
MMP_RS00860: MMP_RS00860 - 50S ribosomal protein L40e, at 161,607 to 161,750
_RS00860
MMP_RS00865: MMP_RS00865 - SLC13 family permease, at 162,132 to 163,472
_RS00865
MMP_RS08930: MMP_RS08930 - hypothetical protein, at 163,741 to 163,899
_RS08930
MMP_RS00870: MMP_RS00870 - homocitrate synthase family protein, at 163,925 to 165,085
_RS00870
Position (kb)
162
163
164 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 161.134 kb on - strand at 161.134 kb on - strand at 161.176 kb on - strand, within MMP_RS00855 at 161.214 kb on - strand, within MMP_RS00855 at 161.260 kb on - strand, within MMP_RS00855 at 161.262 kb on - strand, within MMP_RS00855 at 161.408 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.436 kb on + strand, within MMP_RS00855 at 161.749 kb on + strand at 161.867 kb on + strand at 161.885 kb on + strand at 161.916 kb on - strand at 161.981 kb on + strand at 162.004 kb on + strand at 162.007 kb on + strand at 162.030 kb on + strand at 162.030 kb on + strand at 162.036 kb on + strand at 162.037 kb on - strand at 162.053 kb on + strand at 162.117 kb on + strand at 162.117 kb on + strand at 162.117 kb on + strand at 162.444 kb on + strand, within MMP_RS00865 at 162.604 kb on - strand, within MMP_RS00865 at 162.785 kb on + strand, within MMP_RS00865 at 162.785 kb on + strand, within MMP_RS00865 at 162.788 kb on - strand, within MMP_RS00865 at 163.113 kb on + strand, within MMP_RS00865 at 163.140 kb on - strand, within MMP_RS00865 at 163.360 kb on - strand at 163.420 kb on - strand at 163.513 kb on - strand at 163.562 kb on + strand at 163.566 kb on - strand at 163.567 kb on + strand at 163.569 kb on + strand at 163.569 kb on + strand at 163.579 kb on - strand at 163.580 kb on + strand at 163.580 kb on + strand at 163.621 kb on - strand at 163.622 kb on + strand at 163.626 kb on + strand at 163.644 kb on - strand at 163.645 kb on + strand at 163.648 kb on - strand at 163.654 kb on - strand at 163.655 kb on + strand at 163.684 kb on - strand at 163.685 kb on + strand at 163.742 kb on - strand at 163.777 kb on - strand, within MMP_RS08930 at 163.867 kb on + strand, within MMP_RS08930 at 163.887 kb on - strand at 163.900 kb on + strand at 164.146 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM B-Alanine remove 161,134 - +0.2 161,134 - -1.5 161,176 - MMP_RS00855 0.17 +0.4 161,214 - MMP_RS00855 0.25 +0.3 161,260 - MMP_RS00855 0.34 +0.5 161,262 - MMP_RS00855 0.34 -0.1 161,408 + MMP_RS00855 0.62 +0.9 161,436 + MMP_RS00855 0.68 +0.9 161,436 + MMP_RS00855 0.68 +0.1 161,749 + +0.4 161,867 + +0.5 161,885 + -0.0 161,916 - -0.6 161,981 + -0.1 162,004 + -3.1 162,007 + +0.1 162,030 + -0.8 162,030 + -1.3 162,036 + -2.6 162,037 - +0.3 162,053 + -0.3 162,117 + -0.3 162,117 + -0.1 162,117 + -2.5 162,444 + MMP_RS00865 0.23 +0.4 162,604 - MMP_RS00865 0.35 +0.1 162,785 + MMP_RS00865 0.49 -0.9 162,785 + MMP_RS00865 0.49 -0.1 162,788 - MMP_RS00865 0.49 -2.1 163,113 + MMP_RS00865 0.73 -0.5 163,140 - MMP_RS00865 0.75 -0.0 163,360 - -0.8 163,420 - -0.1 163,513 - -1.0 163,562 + +1.3 163,566 - +0.7 163,567 + -0.5 163,569 + +0.5 163,569 + +0.9 163,579 - -2.2 163,580 + +0.2 163,580 + +0.4 163,621 - -1.4 163,622 + -1.3 163,626 + +0.3 163,644 - -0.8 163,645 + +0.1 163,648 - +0.4 163,654 - -0.7 163,655 + +0.9 163,684 - +0.3 163,685 + +1.2 163,742 - -0.6 163,777 - MMP_RS08930 0.23 +0.3 163,867 + MMP_RS08930 0.79 +0.6 163,887 - -0.4 163,900 + +0.3 164,146 + +2.4
Or see this region's nucleotide sequence