Experiment: H2 N-free with 5mM Inosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05005 and MMP_RS05010 are separated by 256 nucleotides MMP_RS05010 and MMP_RS05015 are separated by 18 nucleotides MMP_RS05015 and MMP_RS05020 are separated by 33 nucleotides MMP_RS05020 and MMP_RS05025 are separated by 28 nucleotides
MMP_RS05005: MMP_RS05005 - uroporphyrinogen-III C-methyltransferase, at 953,174 to 953,893
_RS05005
MMP_RS05010: MMP_RS05010 - KEOPS complex subunit Cgi121, at 954,150 to 954,581
_RS05010
MMP_RS05015: MMP_RS05015 - histidinol dehydrogenase, at 954,600 to 955,874
_RS05015
MMP_RS05020: MMP_RS05020 - hypothetical protein, at 955,908 to 956,324
_RS05020
MMP_RS05025: MMP_RS05025 - ATP-dependent DNA ligase, at 956,353 to 958,074
_RS05025
Position (kb) 954
955
956 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 953.924 kb on - strand at 953.925 kb on + strand at 953.925 kb on + strand at 953.934 kb on - strand at 953.934 kb on - strand at 953.935 kb on + strand at 953.963 kb on + strand at 953.967 kb on + strand at 953.968 kb on - strand at 953.969 kb on + strand at 953.988 kb on - strand at 953.988 kb on - strand at 953.989 kb on + strand at 954.022 kb on + strand at 954.046 kb on + strand at 954.049 kb on - strand at 954.050 kb on + strand at 954.050 kb on + strand at 954.052 kb on + strand at 954.061 kb on - strand at 954.069 kb on - strand at 954.069 kb on - strand at 954.071 kb on - strand at 954.074 kb on - strand at 954.080 kb on + strand at 954.138 kb on + strand at 954.157 kb on - strand at 954.268 kb on - strand at 954.269 kb on + strand at 954.271 kb on + strand at 954.294 kb on + strand at 954.322 kb on - strand at 954.650 kb on + strand at 954.791 kb on + strand at 954.839 kb on + strand at 954.979 kb on + strand at 955.060 kb on - strand at 955.394 kb on - strand at 955.394 kb on - strand at 955.401 kb on + strand at 955.554 kb on - strand at 955.927 kb on - strand at 955.932 kb on + strand at 956.053 kb on - strand, within MMP_RS05020 at 956.131 kb on - strand, within MMP_RS05020 at 956.174 kb on + strand, within MMP_RS05020 at 956.281 kb on - strand, within MMP_RS05020 at 956.281 kb on - strand, within MMP_RS05020 at 956.299 kb on - strand at 956.661 kb on - strand at 956.695 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 5mM Inosine remove 953,924 - -1.8 953,925 + -3.5 953,925 + -4.4 953,934 - -2.0 953,934 - -1.3 953,935 + -0.4 953,963 + -1.3 953,967 + -1.5 953,968 - +1.1 953,969 + +0.1 953,988 - -1.3 953,988 - -0.2 953,989 + +1.5 954,022 + +0.3 954,046 + +0.7 954,049 - -0.6 954,050 + -3.3 954,050 + +2.3 954,052 + -2.0 954,061 - -2.8 954,069 - -3.6 954,069 - -4.4 954,071 - -3.1 954,074 - -4.1 954,080 + -4.0 954,138 + -0.5 954,157 - -2.1 954,268 - -4.1 954,269 + -3.9 954,271 + -4.0 954,294 + -3.4 954,322 - -2.0 954,650 + -2.4 954,791 + -2.4 954,839 + -1.5 954,979 + -3.5 955,060 - -3.1 955,394 - -4.0 955,394 - -1.6 955,401 + -3.7 955,554 - -1.4 955,927 - -1.4 955,932 + +1.0 956,053 - MMP_RS05020 0.35 -0.9 956,131 - MMP_RS05020 0.53 -4.7 956,174 + MMP_RS05020 0.64 -2.4 956,281 - MMP_RS05020 0.89 -2.3 956,281 - MMP_RS05020 0.89 -1.1 956,299 - -1.2 956,661 - -2.8 956,695 + -3.6
Or see this region's nucleotide sequence