Experiment: H2 N-free with 5mM Inosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03225 and MMP_RS03230 are separated by 95 nucleotides MMP_RS03230 and MMP_RS03235 are separated by 213 nucleotides MMP_RS03235 and MMP_RS03240 are separated by 130 nucleotides
MMP_RS03225: MMP_RS03225 - acetolactate decarboxylase, at 608,278 to 609,078
_RS03225
MMP_RS03230: MMP_RS03230 - flavodoxin family protein, at 609,174 to 609,749
_RS03230
MMP_RS03235: MMP_RS03235 - tRNA pseudouridine(13) synthase TruD, at 609,963 to 611,255
_RS03235
MMP_RS03240: MMP_RS03240 - tRNA(Ile)(2)-agmatinylcytidine synthase, at 611,386 to 612,660
_RS03240
Position (kb) 609
610
611
612 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 609.119 kb on + strand at 609.138 kb on - strand at 609.181 kb on - strand at 609.182 kb on + strand at 609.230 kb on + strand at 609.237 kb on + strand, within MMP_RS03230 at 609.283 kb on - strand, within MMP_RS03230 at 609.283 kb on - strand, within MMP_RS03230 at 609.284 kb on + strand, within MMP_RS03230 at 609.315 kb on + strand, within MMP_RS03230 at 609.324 kb on - strand, within MMP_RS03230 at 609.541 kb on - strand, within MMP_RS03230 at 609.548 kb on - strand, within MMP_RS03230 at 609.828 kb on - strand at 609.932 kb on + strand at 609.932 kb on + strand at 609.951 kb on - strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.952 kb on + strand at 609.954 kb on + strand at 609.955 kb on - strand at 609.955 kb on - strand at 609.956 kb on + strand at 609.956 kb on + strand at 610.315 kb on - strand, within MMP_RS03235 at 610.316 kb on + strand, within MMP_RS03235 at 610.316 kb on + strand, within MMP_RS03235 at 610.316 kb on + strand, within MMP_RS03235 at 610.388 kb on - strand, within MMP_RS03235 at 610.532 kb on + strand, within MMP_RS03235 at 610.690 kb on + strand, within MMP_RS03235 at 610.690 kb on + strand, within MMP_RS03235 at 610.787 kb on - strand, within MMP_RS03235 at 611.205 kb on - strand at 611.279 kb on - strand at 611.285 kb on - strand at 611.286 kb on + strand at 611.307 kb on - strand at 611.307 kb on - strand at 611.316 kb on + strand at 611.386 kb on + strand at 611.437 kb on - strand at 611.615 kb on - strand at 611.623 kb on + strand at 611.623 kb on + strand at 611.962 kb on + strand at 612.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 5mM Inosine remove 609,119 + -2.3 609,138 - -0.6 609,181 - -0.9 609,182 + -0.4 609,230 + +3.9 609,237 + MMP_RS03230 0.11 +0.9 609,283 - MMP_RS03230 0.19 +0.5 609,283 - MMP_RS03230 0.19 +2.1 609,284 + MMP_RS03230 0.19 +0.8 609,315 + MMP_RS03230 0.24 +0.2 609,324 - MMP_RS03230 0.26 +3.0 609,541 - MMP_RS03230 0.64 +0.3 609,548 - MMP_RS03230 0.65 -0.6 609,828 - -0.8 609,932 + +1.3 609,932 + +0.2 609,951 - +0.0 609,952 + -0.1 609,952 + +0.1 609,952 + -0.3 609,954 + +3.3 609,955 - -2.9 609,955 - -0.5 609,956 + -0.5 609,956 + +0.3 610,315 - MMP_RS03235 0.27 -0.1 610,316 + MMP_RS03235 0.27 -3.4 610,316 + MMP_RS03235 0.27 -1.4 610,316 + MMP_RS03235 0.27 +1.2 610,388 - MMP_RS03235 0.33 +1.3 610,532 + MMP_RS03235 0.44 -0.8 610,690 + MMP_RS03235 0.56 -0.9 610,690 + MMP_RS03235 0.56 -1.1 610,787 - MMP_RS03235 0.64 -1.7 611,205 - -0.1 611,279 - -1.3 611,285 - +0.1 611,286 + -1.5 611,307 - +1.3 611,307 - +1.4 611,316 + +1.9 611,386 + -4.4 611,437 - -1.8 611,615 - -1.1 611,623 + -1.5 611,623 + -2.7 611,962 + -1.3 612,120 + -1.8
Or see this region's nucleotide sequence