Experiment: H2 N-free with 10mM NAG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01690 and MMP_RS01695 are separated by 367 nucleotides MMP_RS01695 and MMP_RS01700 are separated by 91 nucleotides
MMP_RS01690: MMP_RS01690 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 310,758 to 312,596
_RS01690
MMP_RS01695: MMP_RS01695 - fructose-1 6-bisphosphatase, at 312,964 to 314,115
_RS01695
MMP_RS01700: MMP_RS01700 - dihydroxy-acid dehydratase, at 314,207 to 315,859
_RS01700
Position (kb) 312
313
314
315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 312.075 kb on - strand, within MMP_RS01690 at 312.107 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.314 kb on + strand, within MMP_RS01690 at 312.370 kb on + strand, within MMP_RS01690 at 312.455 kb on - strand at 312.609 kb on - strand at 312.613 kb on + strand at 312.671 kb on + strand at 312.671 kb on + strand at 312.672 kb on - strand at 312.673 kb on + strand at 312.673 kb on + strand at 312.674 kb on - strand at 312.674 kb on - strand at 312.680 kb on + strand at 312.711 kb on + strand at 312.776 kb on + strand at 312.786 kb on + strand at 312.790 kb on - strand at 312.819 kb on - strand at 312.823 kb on - strand at 312.842 kb on - strand at 312.843 kb on + strand at 312.843 kb on + strand at 312.843 kb on + strand at 312.895 kb on + strand at 312.912 kb on - strand at 312.912 kb on - strand at 312.918 kb on - strand at 312.984 kb on + strand at 313.029 kb on + strand at 313.435 kb on - strand at 313.525 kb on - strand at 314.197 kb on - strand at 314.198 kb on + strand at 314.202 kb on - strand at 314.208 kb on - strand at 314.208 kb on - strand at 314.333 kb on - strand at 314.857 kb on - strand, within MMP_RS01700
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM NAG remove 312,075 - MMP_RS01690 0.72 +0.6 312,107 - MMP_RS01690 0.73 -2.2 312,313 - MMP_RS01690 0.85 +0.7 312,313 - MMP_RS01690 0.85 -0.2 312,313 - MMP_RS01690 0.85 -0.4 312,314 + MMP_RS01690 0.85 +2.3 312,370 + MMP_RS01690 0.88 -0.4 312,455 - +0.2 312,609 - -1.4 312,613 + -0.0 312,671 + +1.3 312,671 + +0.2 312,672 - -0.2 312,673 + -0.3 312,673 + -0.0 312,674 - +0.2 312,674 - -1.7 312,680 + +0.0 312,711 + +0.3 312,776 + +1.7 312,786 + -0.1 312,790 - -0.5 312,819 - -0.3 312,823 - +1.2 312,842 - +0.5 312,843 + -0.6 312,843 + -1.4 312,843 + +0.1 312,895 + -1.2 312,912 - -1.9 312,912 - -0.6 312,918 - -2.2 312,984 + -2.3 313,029 + -2.4 313,435 - -0.6 313,525 - -2.8 314,197 - +0.5 314,198 + +0.2 314,202 - -0.3 314,208 - -0.6 314,208 - +0.5 314,333 - -3.1 314,857 - MMP_RS01700 0.39 -2.8
Or see this region's nucleotide sequence