Experiment: H2 N-free with 10mM L-tyrosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03235 and MMP_RS03240 are separated by 130 nucleotides MMP_RS03240 and MMP_RS03245 are separated by 46 nucleotides MMP_RS03245 and MMP_RS03250 are separated by 105 nucleotides
MMP_RS03235: MMP_RS03235 - tRNA pseudouridine(13) synthase TruD, at 609,963 to 611,255
_RS03235
MMP_RS03240: MMP_RS03240 - tRNA(Ile)(2)-agmatinylcytidine synthase, at 611,386 to 612,660
_RS03240
MMP_RS03245: MMP_RS03245 - DNA repair and recombination protein RadB, at 612,707 to 613,357
_RS03245
MMP_RS03250: MMP_RS03250 - MJ1244 family protein, at 613,463 to 613,792
_RS03250
Position (kb) 611
612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 610.388 kb on - strand, within MMP_RS03235 at 610.532 kb on + strand, within MMP_RS03235 at 610.690 kb on + strand, within MMP_RS03235 at 610.690 kb on + strand, within MMP_RS03235 at 610.787 kb on - strand, within MMP_RS03235 at 611.205 kb on - strand at 611.279 kb on - strand at 611.285 kb on - strand at 611.286 kb on + strand at 611.307 kb on - strand at 611.307 kb on - strand at 611.316 kb on + strand at 611.386 kb on + strand at 611.437 kb on - strand at 611.615 kb on - strand at 611.623 kb on + strand at 611.623 kb on + strand at 611.962 kb on + strand at 612.120 kb on + strand at 612.416 kb on - strand at 612.434 kb on + strand at 612.681 kb on + strand at 612.706 kb on - strand at 612.734 kb on + strand at 612.790 kb on - strand, within MMP_RS03245 at 613.003 kb on - strand, within MMP_RS03245 at 613.066 kb on - strand, within MMP_RS03245 at 613.250 kb on + strand, within MMP_RS03245 at 613.253 kb on + strand, within MMP_RS03245 at 613.376 kb on - strand at 613.405 kb on - strand at 613.409 kb on - strand at 613.410 kb on + strand at 613.428 kb on + strand at 613.428 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-tyrosine remove 610,388 - MMP_RS03235 0.33 -1.0 610,532 + MMP_RS03235 0.44 -1.2 610,690 + MMP_RS03235 0.56 -2.9 610,690 + MMP_RS03235 0.56 -0.9 610,787 - MMP_RS03235 0.64 -0.3 611,205 - +1.3 611,279 - +0.0 611,285 - -1.1 611,286 + -2.5 611,307 - -2.4 611,307 - +1.4 611,316 + -0.4 611,386 + -1.5 611,437 - -1.3 611,615 - +2.8 611,623 + -2.5 611,623 + -1.4 611,962 + -1.0 612,120 + -0.7 612,416 - -2.2 612,434 + -0.7 612,681 + +1.6 612,706 - +0.8 612,734 + +0.2 612,790 - MMP_RS03245 0.13 +0.7 613,003 - MMP_RS03245 0.45 -0.8 613,066 - MMP_RS03245 0.55 -0.3 613,250 + MMP_RS03245 0.83 -1.7 613,253 + MMP_RS03245 0.84 -0.3 613,376 - -1.2 613,405 - +0.3 613,409 - +5.1 613,410 + +4.4 613,428 + +6.2 613,428 + +4.0
Or see this region's nucleotide sequence