Experiment: H2 N-free with 10mM L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04030 and MMP_RS04035 are separated by 192 nucleotides MMP_RS04035 and MMP_RS04040 are separated by 5 nucleotides MMP_RS04040 and MMP_RS04045 are separated by 11 nucleotides MMP_RS04045 and MMP_RS04050 are separated by 100 nucleotides
MMP_RS04030: MMP_RS04030 - hypothetical protein, at 765,202 to 765,603
_RS04030
MMP_RS04035: MMP_RS04035 - hypothetical protein, at 765,796 to 766,236
_RS04035
MMP_RS04040: MMP_RS04040 - hypothetical protein, at 766,242 to 766,721
_RS04040
MMP_RS04045: MMP_RS04045 - hypothetical protein, at 766,733 to 767,473
_RS04045
MMP_RS04050: MMP_RS04050 - hypothetical protein, at 767,574 to 767,810
_RS04050
Position (kb) 766
767 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 765.258 kb on - strand, within MMP_RS04030 at 765.258 kb on - strand, within MMP_RS04030 at 765.258 kb on - strand, within MMP_RS04030 at 765.297 kb on - strand, within MMP_RS04030 at 765.392 kb on - strand, within MMP_RS04030 at 765.412 kb on - strand, within MMP_RS04030 at 765.434 kb on + strand, within MMP_RS04030 at 765.506 kb on - strand, within MMP_RS04030 at 765.507 kb on + strand, within MMP_RS04030 at 765.577 kb on - strand at 765.577 kb on - strand at 765.577 kb on - strand at 765.578 kb on + strand at 765.578 kb on + strand at 765.604 kb on + strand at 765.628 kb on + strand at 765.633 kb on + strand at 765.633 kb on + strand at 765.647 kb on - strand at 765.662 kb on - strand at 765.662 kb on - strand at 765.717 kb on - strand at 765.717 kb on - strand at 765.717 kb on - strand at 765.722 kb on + strand at 765.759 kb on + strand at 766.011 kb on + strand, within MMP_RS04035 at 766.082 kb on - strand, within MMP_RS04035 at 766.361 kb on - strand, within MMP_RS04040 at 766.361 kb on - strand, within MMP_RS04040 at 766.362 kb on + strand, within MMP_RS04040 at 766.362 kb on + strand, within MMP_RS04040 at 766.363 kb on - strand, within MMP_RS04040 at 766.364 kb on + strand, within MMP_RS04040 at 766.365 kb on - strand, within MMP_RS04040 at 766.375 kb on + strand, within MMP_RS04040 at 766.413 kb on - strand, within MMP_RS04040 at 766.424 kb on - strand, within MMP_RS04040 at 766.528 kb on - strand, within MMP_RS04040 at 766.528 kb on - strand, within MMP_RS04040 at 766.529 kb on + strand, within MMP_RS04040 at 766.532 kb on - strand, within MMP_RS04040 at 766.626 kb on + strand, within MMP_RS04040 at 766.629 kb on - strand, within MMP_RS04040 at 766.757 kb on + strand at 766.819 kb on + strand, within MMP_RS04045 at 766.897 kb on - strand, within MMP_RS04045 at 766.985 kb on - strand, within MMP_RS04045 at 767.046 kb on + strand, within MMP_RS04045 at 767.053 kb on - strand, within MMP_RS04045 at 767.053 kb on - strand, within MMP_RS04045 at 767.054 kb on + strand, within MMP_RS04045 at 767.145 kb on - strand, within MMP_RS04045 at 767.182 kb on - strand, within MMP_RS04045 at 767.186 kb on - strand, within MMP_RS04045 at 767.199 kb on + strand, within MMP_RS04045 at 767.252 kb on - strand, within MMP_RS04045 at 767.274 kb on + strand, within MMP_RS04045 at 767.303 kb on - strand, within MMP_RS04045 at 767.397 kb on - strand, within MMP_RS04045 at 767.397 kb on - strand, within MMP_RS04045 at 767.398 kb on + strand, within MMP_RS04045 at 767.576 kb on + strand at 767.581 kb on + strand at 767.621 kb on - strand, within MMP_RS04050 at 767.677 kb on - strand, within MMP_RS04050 at 767.712 kb on + strand, within MMP_RS04050
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Cysteine remove 765,258 - MMP_RS04030 0.14 +0.0 765,258 - MMP_RS04030 0.14 -0.1 765,258 - MMP_RS04030 0.14 -0.6 765,297 - MMP_RS04030 0.24 -0.1 765,392 - MMP_RS04030 0.47 -1.7 765,412 - MMP_RS04030 0.52 -0.6 765,434 + MMP_RS04030 0.58 -0.8 765,506 - MMP_RS04030 0.76 +0.6 765,507 + MMP_RS04030 0.76 -0.2 765,577 - +0.1 765,577 - -0.2 765,577 - -0.2 765,578 + +0.6 765,578 + +0.9 765,604 + -0.4 765,628 + -0.2 765,633 + -0.5 765,633 + +0.3 765,647 - +0.4 765,662 - +1.4 765,662 - +0.6 765,717 - -0.5 765,717 - +0.9 765,717 - -0.5 765,722 + +0.9 765,759 + -1.2 766,011 + MMP_RS04035 0.49 +0.1 766,082 - MMP_RS04035 0.65 -0.5 766,361 - MMP_RS04040 0.25 -1.0 766,361 - MMP_RS04040 0.25 +0.3 766,362 + MMP_RS04040 0.25 -0.0 766,362 + MMP_RS04040 0.25 -3.4 766,363 - MMP_RS04040 0.25 +0.4 766,364 + MMP_RS04040 0.25 -2.3 766,365 - MMP_RS04040 0.26 +0.4 766,375 + MMP_RS04040 0.28 +0.3 766,413 - MMP_RS04040 0.36 -0.4 766,424 - MMP_RS04040 0.38 -0.4 766,528 - MMP_RS04040 0.60 -0.2 766,528 - MMP_RS04040 0.60 -1.6 766,529 + MMP_RS04040 0.60 -0.5 766,532 - MMP_RS04040 0.60 +0.2 766,626 + MMP_RS04040 0.80 +0.4 766,629 - MMP_RS04040 0.81 -0.6 766,757 + -0.2 766,819 + MMP_RS04045 0.12 -0.3 766,897 - MMP_RS04045 0.22 +0.7 766,985 - MMP_RS04045 0.34 +0.6 767,046 + MMP_RS04045 0.42 -0.1 767,053 - MMP_RS04045 0.43 +0.2 767,053 - MMP_RS04045 0.43 +0.1 767,054 + MMP_RS04045 0.43 -0.8 767,145 - MMP_RS04045 0.56 +0.4 767,182 - MMP_RS04045 0.61 +0.1 767,186 - MMP_RS04045 0.61 -0.7 767,199 + MMP_RS04045 0.63 +0.3 767,252 - MMP_RS04045 0.70 -0.2 767,274 + MMP_RS04045 0.73 +0.7 767,303 - MMP_RS04045 0.77 -1.0 767,397 - MMP_RS04045 0.90 +0.0 767,397 - MMP_RS04045 0.90 -0.1 767,398 + MMP_RS04045 0.90 +0.0 767,576 + -0.2 767,581 + +0.5 767,621 - MMP_RS04050 0.20 +1.0 767,677 - MMP_RS04050 0.43 -0.3 767,712 + MMP_RS04050 0.58 +0.4
Or see this region's nucleotide sequence