Experiment: H2 N-free with 10mM L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01600 and MMP_RS01605 are separated by 975 nucleotides MMP_RS01605 and MMP_RS01610 are separated by 259 nucleotides MMP_RS01610 and MMP_RS01615 overlap by 1 nucleotides
MMP_RS01600: MMP_RS01600 - 50S ribosomal protein L15e, at 297,985 to 298,569
_RS01600
MMP_RS01605: MMP_RS01605 - hypothetical protein, at 299,545 to 299,793
_RS01605
MMP_RS01610: MMP_RS01610 - hypothetical protein, at 300,053 to 300,514
_RS01610
MMP_RS01615: MMP_RS01615 - hydrogenase maturation nickel metallochaperone HypA, at 300,514 to 300,894
_RS01615
Position (kb) 299
300 Strain fitness (log2 ratio) -1
0
1
2
3 at 298.616 kb on - strand at 298.656 kb on - strand at 298.660 kb on - strand at 298.661 kb on + strand at 298.668 kb on - strand at 298.679 kb on - strand at 298.855 kb on - strand at 298.921 kb on - strand at 298.928 kb on - strand at 298.938 kb on + strand at 298.938 kb on + strand at 298.938 kb on + strand at 298.938 kb on + strand at 298.982 kb on + strand at 299.024 kb on - strand at 299.061 kb on - strand at 299.061 kb on - strand at 299.061 kb on - strand at 299.185 kb on - strand at 299.186 kb on + strand at 299.188 kb on - strand at 299.188 kb on - strand at 299.188 kb on - strand at 299.188 kb on - strand at 299.189 kb on + strand at 299.190 kb on - strand at 299.194 kb on - strand at 299.195 kb on + strand at 299.199 kb on - strand at 299.199 kb on - strand at 299.201 kb on - strand at 299.206 kb on + strand at 299.209 kb on - strand at 299.252 kb on - strand at 299.287 kb on - strand at 299.290 kb on - strand at 299.294 kb on - strand at 299.303 kb on + strand at 299.309 kb on + strand at 299.309 kb on + strand at 299.399 kb on + strand at 299.423 kb on - strand at 299.423 kb on - strand at 299.424 kb on + strand at 299.456 kb on + strand at 299.463 kb on - strand at 299.583 kb on + strand, within MMP_RS01605 at 299.604 kb on - strand, within MMP_RS01605 at 299.606 kb on - strand, within MMP_RS01605 at 299.607 kb on + strand, within MMP_RS01605 at 299.740 kb on + strand, within MMP_RS01605 at 299.758 kb on + strand, within MMP_RS01605 at 299.762 kb on + strand, within MMP_RS01605 at 299.847 kb on + strand at 299.921 kb on + strand at 299.932 kb on - strand at 299.985 kb on - strand at 299.985 kb on - strand at 299.998 kb on - strand at 300.007 kb on - strand at 300.035 kb on + strand at 300.058 kb on + strand at 300.058 kb on + strand at 300.061 kb on - strand at 300.178 kb on + strand, within MMP_RS01610 at 300.252 kb on - strand, within MMP_RS01610 at 300.256 kb on - strand, within MMP_RS01610 at 300.256 kb on - strand, within MMP_RS01610 at 300.351 kb on - strand, within MMP_RS01610 at 300.496 kb on - strand at 300.596 kb on - strand at 300.615 kb on + strand at 300.645 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Cysteine remove 298,616 - +0.5 298,656 - +0.1 298,660 - -0.5 298,661 + +0.8 298,668 - +3.6 298,679 - +0.3 298,855 - +0.2 298,921 - +0.3 298,928 - -0.3 298,938 + +0.5 298,938 + +0.4 298,938 + +0.4 298,938 + +0.5 298,982 + -0.5 299,024 - +0.7 299,061 - +0.1 299,061 - -0.0 299,061 - +0.2 299,185 - -1.0 299,186 + -0.6 299,188 - +0.1 299,188 - +0.6 299,188 - +0.2 299,188 - -0.6 299,189 + -0.3 299,190 - +0.1 299,194 - +0.4 299,195 + +0.3 299,199 - +0.9 299,199 - +0.4 299,201 - -0.6 299,206 + -0.1 299,209 - +0.0 299,252 - +1.6 299,287 - -0.3 299,290 - +0.7 299,294 - +0.4 299,303 + +0.6 299,309 + -1.0 299,309 + +1.2 299,399 + +0.7 299,423 - +0.6 299,423 - -0.4 299,424 + -0.3 299,456 + +0.1 299,463 - +0.3 299,583 + MMP_RS01605 0.15 -0.4 299,604 - MMP_RS01605 0.24 -0.4 299,606 - MMP_RS01605 0.24 -0.3 299,607 + MMP_RS01605 0.25 -0.2 299,740 + MMP_RS01605 0.78 -0.6 299,758 + MMP_RS01605 0.86 +0.4 299,762 + MMP_RS01605 0.87 -0.8 299,847 + +0.2 299,921 + +0.2 299,932 - +0.3 299,985 - +0.3 299,985 - +0.7 299,998 - -0.1 300,007 - -0.1 300,035 + -0.1 300,058 + +1.0 300,058 + -1.2 300,061 - +0.3 300,178 + MMP_RS01610 0.27 +0.6 300,252 - MMP_RS01610 0.43 -0.6 300,256 - MMP_RS01610 0.44 +0.4 300,256 - MMP_RS01610 0.44 -0.1 300,351 - MMP_RS01610 0.65 -1.3 300,496 - -0.5 300,596 - -0.5 300,615 + -0.8 300,645 + -0.2
Or see this region's nucleotide sequence