Experiment: H2 N-free with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04470 and MMP_RS04475 overlap by 35 nucleotides MMP_RS04475 and MMP_RS04480 are separated by 575 nucleotides
MMP_RS04470: MMP_RS04470 - nitrogenase, at 849,999 to 851,375
_RS04470
MMP_RS04475: MMP_RS04475 - nitrogen fixation, at 851,341 to 851,661
_RS04475
MMP_RS04480: MMP_RS04480 - lysine 2 3-aminomutase, at 852,237 to 853,538
_RS04480
Position (kb) 851
852 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 850.360 kb on - strand, within MMP_RS04470 at 850.394 kb on - strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.395 kb on + strand, within MMP_RS04470 at 850.400 kb on - strand, within MMP_RS04470 at 850.401 kb on + strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.408 kb on - strand, within MMP_RS04470 at 850.409 kb on + strand, within MMP_RS04470 at 850.409 kb on + strand, within MMP_RS04470 at 850.480 kb on - strand, within MMP_RS04470 at 850.481 kb on + strand, within MMP_RS04470 at 850.553 kb on + strand, within MMP_RS04470 at 850.562 kb on + strand, within MMP_RS04470 at 850.586 kb on - strand, within MMP_RS04470 at 850.587 kb on + strand, within MMP_RS04470 at 850.587 kb on + strand, within MMP_RS04470 at 850.637 kb on - strand, within MMP_RS04470 at 850.805 kb on - strand, within MMP_RS04470 at 850.838 kb on + strand, within MMP_RS04470 at 850.841 kb on - strand, within MMP_RS04470 at 850.841 kb on - strand, within MMP_RS04470 at 850.934 kb on + strand, within MMP_RS04470 at 851.032 kb on - strand, within MMP_RS04470 at 851.037 kb on + strand, within MMP_RS04470 at 851.052 kb on + strand, within MMP_RS04470 at 851.066 kb on + strand, within MMP_RS04470 at 851.126 kb on + strand, within MMP_RS04470 at 851.180 kb on - strand, within MMP_RS04470 at 851.199 kb on - strand, within MMP_RS04470 at 851.205 kb on + strand, within MMP_RS04470 at 851.239 kb on + strand at 851.240 kb on - strand at 851.241 kb on + strand at 851.260 kb on - strand at 851.261 kb on + strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.264 kb on - strand at 851.267 kb on - strand at 851.268 kb on + strand at 851.268 kb on + strand at 851.269 kb on - strand at 851.269 kb on - strand at 851.276 kb on - strand at 851.293 kb on + strand at 851.382 kb on + strand, within MMP_RS04475 at 851.382 kb on + strand, within MMP_RS04475 at 851.444 kb on - strand, within MMP_RS04475 at 851.484 kb on - strand, within MMP_RS04475 at 851.486 kb on - strand, within MMP_RS04475 at 851.513 kb on + strand, within MMP_RS04475 at 851.934 kb on - strand at 851.944 kb on - strand at 851.945 kb on + strand at 851.957 kb on + strand at 851.966 kb on - strand at 851.971 kb on - strand at 851.972 kb on + strand at 851.975 kb on - strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.976 kb on + strand at 851.998 kb on - strand at 852.002 kb on - strand at 852.041 kb on - strand at 852.046 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.051 kb on - strand at 852.054 kb on + strand at 852.056 kb on - strand at 852.057 kb on + strand at 852.057 kb on + strand at 852.074 kb on + strand at 852.102 kb on - strand at 852.102 kb on - strand at 852.103 kb on + strand at 852.105 kb on + strand at 852.179 kb on - strand at 852.180 kb on + strand at 852.247 kb on - strand at 852.248 kb on + strand at 852.260 kb on - strand at 852.265 kb on - strand at 852.319 kb on + strand at 852.421 kb on - strand, within MMP_RS04480 at 852.422 kb on + strand, within MMP_RS04480 at 852.428 kb on + strand, within MMP_RS04480 at 852.428 kb on + strand, within MMP_RS04480 at 852.443 kb on - strand, within MMP_RS04480 at 852.451 kb on + strand, within MMP_RS04480 at 852.453 kb on - strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.454 kb on + strand, within MMP_RS04480 at 852.463 kb on - strand, within MMP_RS04480 at 852.500 kb on - strand, within MMP_RS04480 at 852.533 kb on + strand, within MMP_RS04480 at 852.533 kb on + strand, within MMP_RS04480 at 852.622 kb on - strand, within MMP_RS04480 at 852.627 kb on - strand, within MMP_RS04480 at 852.632 kb on + strand, within MMP_RS04480
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Threonine remove 850,360 - MMP_RS04470 0.26 -2.2 850,394 - MMP_RS04470 0.29 -1.3 850,395 + MMP_RS04470 0.29 -2.1 850,395 + MMP_RS04470 0.29 -1.6 850,395 + MMP_RS04470 0.29 -2.4 850,400 - MMP_RS04470 0.29 -1.9 850,401 + MMP_RS04470 0.29 -0.6 850,408 - MMP_RS04470 0.30 -1.3 850,408 - MMP_RS04470 0.30 -1.9 850,408 - MMP_RS04470 0.30 -3.2 850,409 + MMP_RS04470 0.30 -1.3 850,409 + MMP_RS04470 0.30 -2.0 850,480 - MMP_RS04470 0.35 -2.2 850,481 + MMP_RS04470 0.35 +0.1 850,553 + MMP_RS04470 0.40 -2.1 850,562 + MMP_RS04470 0.41 -2.1 850,586 - MMP_RS04470 0.43 -2.8 850,587 + MMP_RS04470 0.43 -5.0 850,587 + MMP_RS04470 0.43 -4.1 850,637 - MMP_RS04470 0.46 -0.7 850,805 - MMP_RS04470 0.59 -1.5 850,838 + MMP_RS04470 0.61 -5.0 850,841 - MMP_RS04470 0.61 -2.1 850,841 - MMP_RS04470 0.61 -1.6 850,934 + MMP_RS04470 0.68 -1.3 851,032 - MMP_RS04470 0.75 -1.9 851,037 + MMP_RS04470 0.75 -2.3 851,052 + MMP_RS04470 0.76 -5.0 851,066 + MMP_RS04470 0.77 -3.3 851,126 + MMP_RS04470 0.82 -3.7 851,180 - MMP_RS04470 0.86 -3.4 851,199 - MMP_RS04470 0.87 -1.7 851,205 + MMP_RS04470 0.88 -3.2 851,239 + -1.6 851,240 - -1.8 851,241 + -1.8 851,260 - -4.4 851,261 + -2.5 851,264 - -3.0 851,264 - -2.3 851,264 - -2.3 851,267 - -2.8 851,268 + -1.0 851,268 + -2.9 851,269 - -3.7 851,269 - -2.1 851,276 - +0.1 851,293 + +2.3 851,382 + MMP_RS04475 0.13 +2.0 851,382 + MMP_RS04475 0.13 +4.0 851,444 - MMP_RS04475 0.32 +2.5 851,484 - MMP_RS04475 0.45 +3.7 851,486 - MMP_RS04475 0.45 +3.2 851,513 + MMP_RS04475 0.54 +3.5 851,934 - +0.2 851,944 - +0.2 851,945 + +0.1 851,957 + +0.4 851,966 - -1.7 851,971 - +0.6 851,972 + -0.4 851,975 - -0.9 851,976 + -1.7 851,976 + -1.1 851,976 + +0.4 851,998 - +0.6 852,002 - -0.5 852,041 - +0.4 852,046 - -0.5 852,051 - -0.4 852,051 - +0.3 852,051 - -1.6 852,051 - -1.9 852,054 + +0.5 852,056 - -1.1 852,057 + -1.0 852,057 + -0.3 852,074 + +1.0 852,102 - +0.1 852,102 - -0.7 852,103 + -2.4 852,105 + -1.3 852,179 - +0.9 852,180 + -1.0 852,247 - +0.7 852,248 + +0.7 852,260 - +0.0 852,265 - +0.8 852,319 + -1.3 852,421 - MMP_RS04480 0.14 +1.2 852,422 + MMP_RS04480 0.14 -0.0 852,428 + MMP_RS04480 0.15 +0.1 852,428 + MMP_RS04480 0.15 +0.6 852,443 - MMP_RS04480 0.16 +0.9 852,451 + MMP_RS04480 0.16 -0.6 852,453 - MMP_RS04480 0.17 -1.5 852,454 + MMP_RS04480 0.17 +0.5 852,454 + MMP_RS04480 0.17 -0.7 852,454 + MMP_RS04480 0.17 +0.6 852,463 - MMP_RS04480 0.17 -1.3 852,500 - MMP_RS04480 0.20 -0.2 852,533 + MMP_RS04480 0.23 -0.9 852,533 + MMP_RS04480 0.23 +0.3 852,622 - MMP_RS04480 0.30 +1.4 852,627 - MMP_RS04480 0.30 +0.6 852,632 + MMP_RS04480 0.30 -0.1
Or see this region's nucleotide sequence