Experiment: H2 N-free with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02235 and MMP_RS02240 are separated by 17 nucleotides MMP_RS02240 and MMP_RS02245 are separated by 30 nucleotides MMP_RS02245 and MMP_RS02250 are separated by 132 nucleotides
MMP_RS02235: MMP_RS02235 - CBS domain-containing protein, at 422,287 to 423,252
_RS02235
MMP_RS02240: MMP_RS02240 - methanogenesis marker 7 protein, at 423,270 to 424,184
_RS02240
MMP_RS02245: MMP_RS02245 - hypothetical protein, at 424,215 to 424,667
_RS02245
MMP_RS02250: MMP_RS02250 - FRG domain-containing protein, at 424,800 to 426,188
_RS02250
Position (kb) 423
424
425 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 422.291 kb on - strand at 422.292 kb on + strand at 422.321 kb on - strand at 422.323 kb on - strand at 422.323 kb on - strand at 422.324 kb on + strand at 422.324 kb on + strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.352 kb on - strand at 422.356 kb on - strand at 422.356 kb on - strand at 422.357 kb on + strand at 422.357 kb on + strand at 422.360 kb on - strand at 422.368 kb on - strand at 422.368 kb on - strand at 422.372 kb on - strand at 422.376 kb on - strand at 422.376 kb on - strand at 422.456 kb on - strand, within MMP_RS02235 at 422.456 kb on - strand, within MMP_RS02235 at 422.457 kb on + strand, within MMP_RS02235 at 422.471 kb on - strand, within MMP_RS02235 at 422.472 kb on + strand, within MMP_RS02235 at 422.518 kb on + strand, within MMP_RS02235 at 422.554 kb on + strand, within MMP_RS02235 at 422.623 kb on + strand, within MMP_RS02235 at 422.651 kb on - strand, within MMP_RS02235 at 422.794 kb on + strand, within MMP_RS02235 at 422.829 kb on - strand, within MMP_RS02235 at 422.863 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 423.055 kb on + strand, within MMP_RS02235 at 423.071 kb on - strand, within MMP_RS02235 at 423.076 kb on + strand, within MMP_RS02235 at 423.090 kb on + strand, within MMP_RS02235 at 423.126 kb on + strand, within MMP_RS02235 at 423.126 kb on + strand, within MMP_RS02235 at 423.191 kb on - strand at 423.282 kb on + strand at 423.282 kb on + strand at 423.284 kb on - strand at 423.285 kb on + strand at 423.445 kb on + strand, within MMP_RS02240 at 423.777 kb on - strand, within MMP_RS02240 at 423.885 kb on + strand, within MMP_RS02240 at 423.959 kb on + strand, within MMP_RS02240 at 423.959 kb on + strand, within MMP_RS02240 at 424.077 kb on - strand, within MMP_RS02240 at 424.098 kb on - strand at 424.099 kb on + strand at 424.104 kb on + strand at 424.183 kb on + strand at 424.426 kb on - strand, within MMP_RS02245 at 424.427 kb on + strand, within MMP_RS02245 at 424.435 kb on + strand, within MMP_RS02245 at 424.511 kb on - strand, within MMP_RS02245 at 424.517 kb on + strand, within MMP_RS02245 at 424.657 kb on - strand at 424.657 kb on - strand at 424.657 kb on - strand at 424.659 kb on - strand at 424.744 kb on - strand at 424.745 kb on + strand at 425.010 kb on + strand, within MMP_RS02250 at 425.092 kb on - strand, within MMP_RS02250 at 425.128 kb on - strand, within MMP_RS02250 at 425.145 kb on + strand, within MMP_RS02250 at 425.165 kb on - strand, within MMP_RS02250 at 425.166 kb on + strand, within MMP_RS02250
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Threonine remove 422,291 - -2.0 422,292 + -1.0 422,321 - -0.4 422,323 - -1.4 422,323 - -0.8 422,324 + -1.1 422,324 + -0.5 422,325 - -1.8 422,325 - -0.9 422,325 - -1.3 422,326 + -3.3 422,326 + -1.6 422,326 + -1.0 422,352 - -2.3 422,356 - -3.4 422,356 - -0.7 422,357 + -1.8 422,357 + -0.9 422,360 - -1.6 422,368 - -2.0 422,368 - -2.6 422,372 - -1.1 422,376 - -1.4 422,376 - -0.2 422,456 - MMP_RS02235 0.17 -2.8 422,456 - MMP_RS02235 0.17 -3.3 422,457 + MMP_RS02235 0.18 -4.8 422,471 - MMP_RS02235 0.19 -1.0 422,472 + MMP_RS02235 0.19 -0.8 422,518 + MMP_RS02235 0.24 -1.4 422,554 + MMP_RS02235 0.28 -3.4 422,623 + MMP_RS02235 0.35 -2.8 422,651 - MMP_RS02235 0.38 -1.0 422,794 + MMP_RS02235 0.52 -2.2 422,829 - MMP_RS02235 0.56 -1.3 422,863 - MMP_RS02235 0.60 -1.4 422,938 - MMP_RS02235 0.67 -1.7 422,938 - MMP_RS02235 0.67 -1.2 423,055 + MMP_RS02235 0.80 -1.3 423,071 - MMP_RS02235 0.81 -1.5 423,076 + MMP_RS02235 0.82 -1.0 423,090 + MMP_RS02235 0.83 -1.5 423,126 + MMP_RS02235 0.87 -2.3 423,126 + MMP_RS02235 0.87 -1.6 423,191 - -2.7 423,282 + -2.0 423,282 + -1.6 423,284 - +0.6 423,285 + -1.9 423,445 + MMP_RS02240 0.19 -2.1 423,777 - MMP_RS02240 0.55 -3.4 423,885 + MMP_RS02240 0.67 -1.4 423,959 + MMP_RS02240 0.75 -2.6 423,959 + MMP_RS02240 0.75 -2.7 424,077 - MMP_RS02240 0.88 -2.3 424,098 - -2.5 424,099 + -1.4 424,104 + -1.6 424,183 + -0.6 424,426 - MMP_RS02245 0.47 -3.5 424,427 + MMP_RS02245 0.47 -1.3 424,435 + MMP_RS02245 0.49 -2.1 424,511 - MMP_RS02245 0.65 -2.4 424,517 + MMP_RS02245 0.67 -3.1 424,657 - -1.5 424,657 - -2.1 424,657 - -1.1 424,659 - -2.4 424,744 - +1.3 424,745 + +0.3 425,010 + MMP_RS02250 0.15 +0.5 425,092 - MMP_RS02250 0.21 +1.2 425,128 - MMP_RS02250 0.24 +0.8 425,145 + MMP_RS02250 0.25 +1.4 425,165 - MMP_RS02250 0.26 -1.0 425,166 + MMP_RS02250 0.26 -1.1
Or see this region's nucleotide sequence