Experiment: H2 N-free with 10mM L-Glutamic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05730 and MMP_RS05735 are separated by 28 nucleotides MMP_RS05735 and MMP_RS05740 overlap by 14 nucleotides
MMP_RS05730: MMP_RS05730 - DUF373 family protein, at 1,099,029 to 1,100,138
_RS05730
MMP_RS05735: MMP_RS05735 - oligosaccharide repeat unit polymerase family protein, at 1,100,167 to 1,101,342
_RS05735
MMP_RS05740: MMP_RS05740 - oligosaccharide repeat unit polymerase family protein, at 1,101,329 to 1,102,402
_RS05740
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1099.287 kb on - strand at 1099.365 kb on - strand at 1099.405 kb on + strand at 1099.663 kb on - strand at 1099.781 kb on + strand at 1099.810 kb on + strand at 1099.863 kb on - strand at 1099.863 kb on - strand at 1099.863 kb on - strand at 1099.864 kb on + strand at 1099.864 kb on + strand at 1099.864 kb on + strand at 1100.030 kb on - strand at 1100.295 kb on + strand at 1100.310 kb on - strand at 1100.311 kb on + strand at 1100.332 kb on - strand at 1100.894 kb on + strand at 1100.960 kb on - strand at 1100.980 kb on - strand at 1101.022 kb on - strand at 1101.366 kb on - strand at 1101.448 kb on + strand at 1101.617 kb on - strand at 1101.897 kb on - strand at 1102.070 kb on - strand at 1102.071 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Glutamic remove 1,099,287 - -3.6 1,099,365 - -2.4 1,099,405 + -4.5 1,099,663 - -2.1 1,099,781 + -4.2 1,099,810 + -1.2 1,099,863 - -2.5 1,099,863 - -1.8 1,099,863 - -2.9 1,099,864 + -2.9 1,099,864 + +0.2 1,099,864 + -4.7 1,100,030 - -2.1 1,100,295 + -4.5 1,100,310 - -3.9 1,100,311 + -2.1 1,100,332 - -1.6 1,100,894 + +0.3 1,100,960 - -4.9 1,100,980 - -2.6 1,101,022 - -3.1 1,101,366 - +0.9 1,101,448 + +0.2 1,101,617 - -1.8 1,101,897 - -2.0 1,102,070 - -1.8 1,102,071 + -0.4
Or see this region's nucleotide sequence