Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08765 and MMP_RS08985 are separated by 96 nucleotides MMP_RS08985 and MMP_RS08770 are separated by 22 nucleotides MMP_RS08770 and MMP_RS08775 overlap by 4 nucleotides
MMP_RS08765: MMP_RS08765 - homoserine dehydrogenase, at 1,642,986 to 1,643,999
_RS08765
MMP_RS08985: MMP_RS08985 - tRNA-Sec, at 1,644,096 to 1,644,185
_RS08985
MMP_RS08770: MMP_RS08770 - hypothetical protein, at 1,644,208 to 1,645,104
_RS08770
MMP_RS08775: MMP_RS08775 - methanogenesis marker 9 domain-containing protein, at 1,645,101 to 1,645,556
_RS08775
Position (kb)
1644
1645 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1643.153 kb on + strand at 1643.315 kb on + strand at 1643.379 kb on + strand at 1643.425 kb on + strand at 1644.059 kb on - strand at 1644.059 kb on - strand at 1644.060 kb on + strand at 1644.062 kb on + strand at 1644.063 kb on - strand at 1644.261 kb on + strand at 1644.288 kb on - strand at 1644.408 kb on + strand, within MMP_RS08770 at 1644.417 kb on + strand, within MMP_RS08770 at 1644.441 kb on - strand, within MMP_RS08770 at 1644.563 kb on - strand, within MMP_RS08770 at 1644.571 kb on + strand, within MMP_RS08770 at 1644.683 kb on - strand, within MMP_RS08770 at 1644.684 kb on + strand, within MMP_RS08770 at 1644.710 kb on - strand, within MMP_RS08770 at 1644.769 kb on + strand, within MMP_RS08770 at 1644.861 kb on + strand, within MMP_RS08770 at 1644.867 kb on - strand, within MMP_RS08770 at 1644.870 kb on + strand, within MMP_RS08770 at 1645.014 kb on - strand, within MMP_RS08770 at 1645.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,643,153 + -2.7 1,643,315 + -3.8 1,643,379 + -3.7 1,643,425 + -2.5 1,644,059 - -0.7 1,644,059 - -0.5 1,644,060 + -0.3 1,644,062 + +0.1 1,644,063 - -2.7 1,644,261 + +0.0 1,644,288 - +0.3 1,644,408 + MMP_RS08770 0.22 -0.4 1,644,417 + MMP_RS08770 0.23 -0.5 1,644,441 - MMP_RS08770 0.26 +0.6 1,644,563 - MMP_RS08770 0.40 -1.0 1,644,571 + MMP_RS08770 0.40 +0.6 1,644,683 - MMP_RS08770 0.53 +0.2 1,644,684 + MMP_RS08770 0.53 -1.2 1,644,710 - MMP_RS08770 0.56 +1.5 1,644,769 + MMP_RS08770 0.63 +1.0 1,644,861 + MMP_RS08770 0.73 +0.9 1,644,867 - MMP_RS08770 0.73 +0.7 1,644,870 + MMP_RS08770 0.74 +0.7 1,645,014 - MMP_RS08770 0.90 +1.0 1,645,088 - +0.3
Or see this region's nucleotide sequence