Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08795 and MMP_RS08800 are separated by 22 nucleotides MMP_RS08800 and MMP_RS08805 overlap by 44 nucleotides MMP_RS08805 and MMP_RS08810 are separated by 32 nucleotides MMP_RS08810 and MMP_RS08815 are separated by 74 nucleotides
MMP_RS08795: MMP_RS08795 - 30S ribosomal protein S27e, at 1,647,312 to 1,647,497
_RS08795
MMP_RS08800: MMP_RS08800 - 50S ribosomal protein L44e, at 1,647,520 to 1,647,801
_RS08800
MMP_RS08805: MMP_RS08805 - hypothetical protein, at 1,647,758 to 1,648,345
_RS08805
MMP_RS08810: MMP_RS08810 - DNA polymerase sliding clamp, at 1,648,378 to 1,649,130
_RS08810
MMP_RS08815: MMP_RS08815 - LysR family transcriptional regulator, at 1,649,205 to 1,650,095
_RS08815
Position (kb)
1648
1649
1650 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1648.360 kb on - strand at 1648.361 kb on + strand at 1649.069 kb on + strand at 1649.109 kb on - strand at 1649.112 kb on - strand at 1649.167 kb on + strand at 1649.180 kb on + strand at 1649.279 kb on + strand at 1649.374 kb on + strand at 1649.399 kb on - strand at 1649.440 kb on + strand at 1649.484 kb on - strand at 1649.485 kb on + strand at 1649.496 kb on + strand at 1649.535 kb on - strand at 1649.661 kb on - strand at 1649.750 kb on - strand at 1649.752 kb on - strand at 1649.766 kb on + strand at 1649.775 kb on - strand at 1649.792 kb on - strand at 1649.897 kb on + strand at 1649.920 kb on + strand at 1649.985 kb on + strand at 1650.104 kb on - strand at 1650.125 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,648,360 - -1.1 1,648,361 + -0.9 1,649,069 + -3.9 1,649,109 - -1.1 1,649,112 - -1.7 1,649,167 + -2.1 1,649,180 + -1.7 1,649,279 + -3.5 1,649,374 + -4.8 1,649,399 - -5.2 1,649,440 + -1.9 1,649,484 - -4.5 1,649,485 + -3.5 1,649,496 + -2.2 1,649,535 - -3.8 1,649,661 - -3.7 1,649,750 - -2.7 1,649,752 - -5.5 1,649,766 + -4.8 1,649,775 - -4.6 1,649,792 - -5.3 1,649,897 + -1.7 1,649,920 + -3.5 1,649,985 + -2.0 1,650,104 - -4.1 1,650,125 + -2.7
Or see this region's nucleotide sequence