Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08785 and MMP_RS08790 are separated by 8 nucleotides MMP_RS08790 and MMP_RS08795 are separated by 113 nucleotides MMP_RS08795 and MMP_RS08800 are separated by 22 nucleotides MMP_RS08800 and MMP_RS08805 overlap by 44 nucleotides MMP_RS08805 and MMP_RS08810 are separated by 32 nucleotides
MMP_RS08785: MMP_RS08785 - RNA-protein complex protein Nop10, at 1,646,237 to 1,646,392
_RS08785
MMP_RS08790: MMP_RS08790 - translation initiation factor IF-2 subunit alpha, at 1,646,401 to 1,647,198
_RS08790
MMP_RS08795: MMP_RS08795 - 30S ribosomal protein S27e, at 1,647,312 to 1,647,497
_RS08795
MMP_RS08800: MMP_RS08800 - 50S ribosomal protein L44e, at 1,647,520 to 1,647,801
_RS08800
MMP_RS08805: MMP_RS08805 - hypothetical protein, at 1,647,758 to 1,648,345
_RS08805
MMP_RS08810: MMP_RS08810 - DNA polymerase sliding clamp, at 1,648,378 to 1,649,130
_RS08810
Position (kb)
1647
1648 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1646.524 kb on - strand, within MMP_RS08790 at 1646.550 kb on + strand, within MMP_RS08790 at 1646.713 kb on - strand, within MMP_RS08790 at 1647.112 kb on - strand, within MMP_RS08790 at 1647.218 kb on - strand at 1647.219 kb on + strand at 1647.223 kb on - strand at 1647.224 kb on + strand at 1647.230 kb on + strand at 1647.240 kb on - strand at 1647.243 kb on + strand at 1647.250 kb on + strand at 1647.277 kb on - strand at 1647.278 kb on + strand at 1647.283 kb on - strand at 1647.304 kb on + strand at 1647.313 kb on - strand at 1647.319 kb on + strand at 1647.322 kb on + strand at 1648.360 kb on - strand at 1648.361 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,646,524 - MMP_RS08790 0.15 -0.1 1,646,550 + MMP_RS08790 0.19 -1.4 1,646,713 - MMP_RS08790 0.39 -2.5 1,647,112 - MMP_RS08790 0.89 -0.1 1,647,218 - -2.3 1,647,219 + -4.0 1,647,223 - -3.6 1,647,224 + -3.6 1,647,230 + -2.7 1,647,240 - -3.5 1,647,243 + -2.9 1,647,250 + -4.8 1,647,277 - +0.0 1,647,278 + +0.4 1,647,283 - -1.4 1,647,304 + +0.6 1,647,313 - -0.7 1,647,319 + -0.1 1,647,322 + -0.3 1,648,360 - -1.1 1,648,361 + -0.9
Or see this region's nucleotide sequence