Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08725 and MMP_RS08730 are separated by 41 nucleotides MMP_RS08730 and MMP_RS08735 are separated by 99 nucleotides MMP_RS08735 and MMP_RS08740 are separated by 187 nucleotides
MMP_RS08725: MMP_RS08725 - Ni/Fe hydrogenase subunit alpha, at 1,634,132 to 1,635,388
_RS08725
MMP_RS08730: MMP_RS08730 - F420-non-reducing hydrogenase subunit, at 1,635,430 to 1,636,296
_RS08730
MMP_RS08735: MMP_RS08735 - hydrogenase iron-sulfur subunit, at 1,636,396 to 1,636,800
_RS08735
MMP_RS08740: MMP_RS08740 - CoB--CoM heterodisulfide reductase iron-sulfur subunit A family protein, at 1,636,988 to 1,638,964
_RS08740
Position (kb)
1635
1636
1637 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1634.721 kb on - strand, within MMP_RS08725 at 1635.324 kb on + strand at 1635.424 kb on + strand at 1635.892 kb on + strand at 1635.999 kb on + strand at 1636.112 kb on + strand at 1636.783 kb on + strand at 1637.178 kb on - strand at 1637.198 kb on - strand at 1637.199 kb on + strand at 1637.199 kb on + strand at 1637.217 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,634,721 - MMP_RS08725 0.47 -3.8 1,635,324 + -1.7 1,635,424 + -5.0 1,635,892 + -4.1 1,635,999 + -3.7 1,636,112 + -4.8 1,636,783 + -3.1 1,637,178 - -2.9 1,637,198 - -1.1 1,637,199 + -1.5 1,637,199 + -1.9 1,637,217 + -3.5
Or see this region's nucleotide sequence