Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08550 and MMP_RS08555 overlap by 11 nucleotides MMP_RS08555 and MMP_RS08560 overlap by 4 nucleotides MMP_RS08560 and MMP_RS08565 are separated by 29 nucleotides MMP_RS08565 and MMP_RS08570 are separated by 50 nucleotides MMP_RS08570 and MMP_RS08575 are separated by 32 nucleotides
MMP_RS08550: MMP_RS08550 - DUF2304 domain-containing protein, at 1,604,456 to 1,604,815
_RS08550
MMP_RS08555: MMP_RS08555 - hypothetical protein, at 1,604,805 to 1,605,002
_RS08555
MMP_RS08560: MMP_RS08560 - precorrin-4 C(11)-methyltransferase, at 1,604,999 to 1,605,763
_RS08560
MMP_RS08565: MMP_RS08565 - aconitase X catalytic domain-containing protein, at 1,605,793 to 1,606,998
_RS08565
MMP_RS08570: MMP_RS08570 - coenzyme F420-0:L-glutamate ligase, at 1,607,049 to 1,607,945
_RS08570
MMP_RS08575: MMP_RS08575 - hypothetical protein, at 1,607,978 to 1,609,657
_RS08575
Position (kb)
1605
1606
1607 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1604.842 kb on + strand, within MMP_RS08555 at 1604.926 kb on - strand, within MMP_RS08555 at 1604.928 kb on - strand, within MMP_RS08555 at 1604.929 kb on + strand, within MMP_RS08555 at 1605.073 kb on - strand at 1605.273 kb on - strand at 1605.287 kb on + strand at 1605.287 kb on + strand at 1605.629 kb on + strand at 1605.629 kb on + strand at 1605.631 kb on + strand at 1605.635 kb on - strand at 1605.866 kb on - strand at 1606.243 kb on - strand at 1606.641 kb on - strand at 1607.012 kb on - strand at 1607.021 kb on + strand at 1607.026 kb on - strand at 1607.056 kb on - strand at 1607.109 kb on - strand at 1607.109 kb on - strand at 1607.204 kb on + strand, within MMP_RS08570 at 1607.289 kb on + strand, within MMP_RS08570 at 1607.292 kb on - strand, within MMP_RS08570 at 1607.293 kb on + strand, within MMP_RS08570 at 1607.295 kb on - strand, within MMP_RS08570 at 1607.296 kb on + strand, within MMP_RS08570 at 1607.296 kb on + strand, within MMP_RS08570 at 1607.296 kb on + strand, within MMP_RS08570 at 1607.296 kb on + strand, within MMP_RS08570 at 1607.338 kb on - strand, within MMP_RS08570 at 1607.426 kb on + strand, within MMP_RS08570 at 1607.435 kb on + strand, within MMP_RS08570 at 1607.436 kb on - strand, within MMP_RS08570 at 1607.493 kb on - strand, within MMP_RS08570 at 1607.494 kb on + strand, within MMP_RS08570 at 1607.659 kb on - strand, within MMP_RS08570 at 1607.754 kb on + strand, within MMP_RS08570 at 1607.885 kb on + strand at 1607.924 kb on + strand at 1607.975 kb on - strand at 1607.975 kb on - strand at 1607.976 kb on + strand at 1607.976 kb on + strand at 1607.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,604,842 + MMP_RS08555 0.19 +0.6 1,604,926 - MMP_RS08555 0.61 +0.6 1,604,928 - MMP_RS08555 0.62 +0.4 1,604,929 + MMP_RS08555 0.63 +0.2 1,605,073 - -3.2 1,605,273 - -2.7 1,605,287 + -1.2 1,605,287 + -1.6 1,605,629 + -3.2 1,605,629 + -1.3 1,605,631 + -2.9 1,605,635 - -3.3 1,605,866 - -4.5 1,606,243 - -0.2 1,606,641 - -3.0 1,607,012 - +0.9 1,607,021 + -0.3 1,607,026 - +0.3 1,607,056 - +0.5 1,607,109 - -1.0 1,607,109 - -2.4 1,607,204 + MMP_RS08570 0.17 -1.4 1,607,289 + MMP_RS08570 0.27 -1.4 1,607,292 - MMP_RS08570 0.27 -4.0 1,607,293 + MMP_RS08570 0.27 -1.6 1,607,295 - MMP_RS08570 0.27 -0.6 1,607,296 + MMP_RS08570 0.28 -1.4 1,607,296 + MMP_RS08570 0.28 -3.1 1,607,296 + MMP_RS08570 0.28 -2.8 1,607,296 + MMP_RS08570 0.28 -2.6 1,607,338 - MMP_RS08570 0.32 -2.4 1,607,426 + MMP_RS08570 0.42 -2.0 1,607,435 + MMP_RS08570 0.43 -2.8 1,607,436 - MMP_RS08570 0.43 -2.4 1,607,493 - MMP_RS08570 0.49 -2.0 1,607,494 + MMP_RS08570 0.50 -1.0 1,607,659 - MMP_RS08570 0.68 -1.2 1,607,754 + MMP_RS08570 0.79 -2.7 1,607,885 + -3.3 1,607,924 + -1.7 1,607,975 - +0.4 1,607,975 - -0.9 1,607,976 + -0.7 1,607,976 + +0.2 1,607,979 - +0.6
Or see this region's nucleotide sequence