Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08535 and MMP_RS08540 are separated by 52 nucleotides MMP_RS08540 and MMP_RS08545 are separated by 159 nucleotides MMP_RS08545 and MMP_RS08550 are separated by 85 nucleotides MMP_RS08550 and MMP_RS08555 overlap by 11 nucleotides MMP_RS08555 and MMP_RS08560 overlap by 4 nucleotides
MMP_RS08535: MMP_RS08535 - bis-aminopropyl spermidine synthase family protein, at 1,601,691 to 1,602,863
_RS08535
MMP_RS08540: MMP_RS08540 - 30S ribosomal protein S8e, at 1,602,916 to 1,603,302
_RS08540
MMP_RS08545: MMP_RS08545 - aspartate carbamoyltransferase, at 1,603,462 to 1,604,370
_RS08545
MMP_RS08550: MMP_RS08550 - DUF2304 domain-containing protein, at 1,604,456 to 1,604,815
_RS08550
MMP_RS08555: MMP_RS08555 - hypothetical protein, at 1,604,805 to 1,605,002
_RS08555
MMP_RS08560: MMP_RS08560 - precorrin-4 C(11)-methyltransferase, at 1,604,999 to 1,605,763
_RS08560
Position (kb)
1603
1604
1605 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1602.504 kb on - strand, within MMP_RS08535 at 1602.567 kb on + strand, within MMP_RS08535 at 1602.582 kb on - strand, within MMP_RS08535 at 1602.769 kb on - strand at 1602.903 kb on - strand at 1602.917 kb on - strand at 1602.918 kb on + strand at 1602.928 kb on - strand at 1602.929 kb on + strand at 1603.073 kb on + strand at 1603.230 kb on - strand at 1603.352 kb on - strand at 1603.355 kb on + strand at 1603.356 kb on - strand at 1603.356 kb on - strand at 1603.357 kb on + strand at 1603.386 kb on - strand at 1603.391 kb on - strand at 1603.392 kb on + strand at 1603.392 kb on + strand at 1603.408 kb on + strand at 1603.665 kb on + strand at 1603.902 kb on - strand at 1604.397 kb on - strand at 1604.400 kb on + strand at 1604.409 kb on + strand at 1604.410 kb on - strand at 1604.411 kb on + strand at 1604.413 kb on + strand at 1604.414 kb on - strand at 1604.421 kb on + strand at 1604.427 kb on - strand at 1604.428 kb on + strand at 1604.428 kb on + strand at 1604.432 kb on - strand at 1604.433 kb on + strand at 1604.433 kb on + strand at 1604.433 kb on + strand at 1604.433 kb on + strand at 1604.436 kb on - strand at 1604.437 kb on + strand at 1604.437 kb on + strand at 1604.842 kb on + strand, within MMP_RS08555 at 1604.926 kb on - strand, within MMP_RS08555 at 1604.928 kb on - strand, within MMP_RS08555 at 1604.929 kb on + strand, within MMP_RS08555 at 1605.073 kb on - strand at 1605.273 kb on - strand at 1605.287 kb on + strand at 1605.287 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,602,504 - MMP_RS08535 0.69 +0.6 1,602,567 + MMP_RS08535 0.75 +1.3 1,602,582 - MMP_RS08535 0.76 +0.2 1,602,769 - -1.0 1,602,903 - -0.7 1,602,917 - -0.0 1,602,918 + +0.2 1,602,928 - -1.0 1,602,929 + +0.2 1,603,073 + -2.7 1,603,230 - -1.3 1,603,352 - -3.2 1,603,355 + -1.4 1,603,356 - -2.0 1,603,356 - -3.7 1,603,357 + -1.7 1,603,386 - +0.1 1,603,391 - -4.5 1,603,392 + -2.4 1,603,392 + -1.5 1,603,408 + -0.8 1,603,665 + -3.7 1,603,902 - -3.0 1,604,397 - -0.8 1,604,400 + +0.2 1,604,409 + -0.1 1,604,410 - +1.0 1,604,411 + -4.5 1,604,413 + -1.2 1,604,414 - +0.7 1,604,421 + +0.1 1,604,427 - +0.3 1,604,428 + -0.1 1,604,428 + -0.5 1,604,432 - +0.7 1,604,433 + +0.8 1,604,433 + +0.7 1,604,433 + -0.2 1,604,433 + -0.3 1,604,436 - +1.2 1,604,437 + +0.6 1,604,437 + +0.6 1,604,842 + MMP_RS08555 0.19 +0.6 1,604,926 - MMP_RS08555 0.61 +0.6 1,604,928 - MMP_RS08555 0.62 +0.4 1,604,929 + MMP_RS08555 0.63 +0.2 1,605,073 - -3.2 1,605,273 - -2.7 1,605,287 + -1.2 1,605,287 + -1.6
Or see this region's nucleotide sequence