Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08460 and MMP_RS08465 are separated by 54 nucleotides MMP_RS08465 and MMP_RS08470 are separated by 4 nucleotides
MMP_RS08460: MMP_RS08460 - proteasome-activating nucleotidase, at 1,587,504 to 1,588,727
_RS08460
MMP_RS08465: MMP_RS08465 - NAD(P)-binding protein, at 1,588,782 to 1,589,972
_RS08465
MMP_RS08470: MMP_RS08470 - ATP-binding cassette domain-containing protein, at 1,589,977 to 1,591,038
_RS08470
Position (kb)
1588
1589
1590 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1588.242 kb on + strand, within MMP_RS08460 at 1588.367 kb on + strand, within MMP_RS08460 at 1588.529 kb on + strand, within MMP_RS08460 at 1588.729 kb on - strand at 1589.275 kb on - strand at 1589.453 kb on - strand at 1589.456 kb on + strand at 1589.461 kb on - strand at 1589.726 kb on + strand at 1590.013 kb on - strand at 1590.020 kb on + strand at 1590.073 kb on - strand at 1590.202 kb on + strand, within MMP_RS08470 at 1590.226 kb on - strand, within MMP_RS08470 at 1590.454 kb on + strand, within MMP_RS08470 at 1590.592 kb on - strand, within MMP_RS08470 at 1590.593 kb on + strand, within MMP_RS08470 at 1590.692 kb on + strand, within MMP_RS08470 at 1590.812 kb on + strand, within MMP_RS08470 at 1590.841 kb on - strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.842 kb on + strand, within MMP_RS08470 at 1590.862 kb on - strand, within MMP_RS08470 at 1590.947 kb on + strand at 1590.950 kb on - strand at 1590.951 kb on + strand at 1590.970 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,588,242 + MMP_RS08460 0.60 +0.5 1,588,367 + MMP_RS08460 0.71 -2.2 1,588,529 + MMP_RS08460 0.84 -0.1 1,588,729 - -0.9 1,589,275 - -0.7 1,589,453 - -2.5 1,589,456 + -3.7 1,589,461 - -0.4 1,589,726 + +0.6 1,590,013 - +0.6 1,590,020 + +0.4 1,590,073 - +0.4 1,590,202 + MMP_RS08470 0.21 -0.1 1,590,226 - MMP_RS08470 0.23 -0.2 1,590,454 + MMP_RS08470 0.45 +0.4 1,590,592 - MMP_RS08470 0.58 +1.0 1,590,593 + MMP_RS08470 0.58 +0.7 1,590,692 + MMP_RS08470 0.67 -0.2 1,590,812 + MMP_RS08470 0.79 -0.5 1,590,841 - MMP_RS08470 0.81 +1.2 1,590,842 + MMP_RS08470 0.81 +0.3 1,590,842 + MMP_RS08470 0.81 -0.1 1,590,862 - MMP_RS08470 0.83 -0.2 1,590,947 + +0.3 1,590,950 - -0.6 1,590,951 + +0.3 1,590,970 - +0.6
Or see this region's nucleotide sequence