Strain Fitness in Methanococcus maripaludis S2 around MMP_RS08425

Experiment: H2 N-free with 10mM L-Aspartic Acid

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS08420 and MMP_RS08425 are separated by 129 nucleotidesMMP_RS08425 and MMP_RS08430 overlap by 11 nucleotidesMMP_RS08430 and MMP_RS08435 are separated by 76 nucleotides MMP_RS08420: MMP_RS08420 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,580,669 to 1,581,871 _RS08420 MMP_RS08425: MMP_RS08425 - methionine adenosyltransferase, at 1,582,001 to 1,583,218 _RS08425 MMP_RS08430: MMP_RS08430 - HAD family hydrolase, at 1,583,208 to 1,584,014 _RS08430 MMP_RS08435: MMP_RS08435 - Era-like GTP-binding protein, at 1,584,091 to 1,584,645 _RS08435 Position (kb) 1582 1583 1584Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1581.554 kb on + strandat 1581.950 kb on + strandat 1581.957 kb on - strandat 1582.522 kb on - strandat 1582.677 kb on + strandat 1583.206 kb on + strandat 1583.206 kb on + strandat 1583.209 kb on - strandat 1583.209 kb on - strandat 1583.209 kb on - strandat 1583.210 kb on + strandat 1583.210 kb on + strandat 1583.217 kb on + strandat 1583.253 kb on - strandat 1583.254 kb on + strandat 1583.256 kb on + strandat 1583.263 kb on + strandat 1583.347 kb on + strand, within MMP_RS08430at 1583.621 kb on - strand, within MMP_RS08430at 1583.622 kb on + strand, within MMP_RS08430at 1583.624 kb on + strand, within MMP_RS08430at 1583.624 kb on + strand, within MMP_RS08430at 1583.628 kb on - strand, within MMP_RS08430at 1583.646 kb on + strand, within MMP_RS08430at 1583.646 kb on + strand, within MMP_RS08430at 1583.967 kb on - strandat 1583.973 kb on - strandat 1583.974 kb on + strandat 1584.034 kb on + strandat 1584.036 kb on - strandat 1584.037 kb on + strandat 1584.037 kb on + strandat 1584.055 kb on - strandat 1584.058 kb on - strandat 1584.060 kb on - strandat 1584.068 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid
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1,581,554 + -1.5
1,581,950 + +1.3
1,581,957 - -1.8
1,582,522 - -4.0
1,582,677 + -3.2
1,583,206 + -0.2
1,583,206 + -0.3
1,583,209 - +1.0
1,583,209 - +0.6
1,583,209 - +0.7
1,583,210 + +1.6
1,583,210 + -0.4
1,583,217 + +0.6
1,583,253 - +0.4
1,583,254 + +0.2
1,583,256 + +1.0
1,583,263 + +0.2
1,583,347 + MMP_RS08430 0.17 +0.2
1,583,621 - MMP_RS08430 0.51 +0.2
1,583,622 + MMP_RS08430 0.51 +2.2
1,583,624 + MMP_RS08430 0.52 +0.7
1,583,624 + MMP_RS08430 0.52 -0.6
1,583,628 - MMP_RS08430 0.52 -0.2
1,583,646 + MMP_RS08430 0.54 +1.1
1,583,646 + MMP_RS08430 0.54 -1.2
1,583,967 - +0.4
1,583,973 - +0.4
1,583,974 + +0.7
1,584,034 + +1.5
1,584,036 - +1.2
1,584,037 + +0.1
1,584,037 + +0.1
1,584,055 - -0.0
1,584,058 - +0.3
1,584,060 - +0.0
1,584,068 + -0.4

Or see this region's nucleotide sequence