Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08420 and MMP_RS08425 are separated by 129 nucleotides MMP_RS08425 and MMP_RS08430 overlap by 11 nucleotides MMP_RS08430 and MMP_RS08435 are separated by 76 nucleotides
MMP_RS08420: MMP_RS08420 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,580,669 to 1,581,871
_RS08420
MMP_RS08425: MMP_RS08425 - methionine adenosyltransferase, at 1,582,001 to 1,583,218
_RS08425
MMP_RS08430: MMP_RS08430 - HAD family hydrolase, at 1,583,208 to 1,584,014
_RS08430
MMP_RS08435: MMP_RS08435 - Era-like GTP-binding protein, at 1,584,091 to 1,584,645
_RS08435
Position (kb)
1582
1583
1584 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1581.554 kb on + strand at 1581.950 kb on + strand at 1581.957 kb on - strand at 1582.522 kb on - strand at 1582.677 kb on + strand at 1583.206 kb on + strand at 1583.206 kb on + strand at 1583.209 kb on - strand at 1583.209 kb on - strand at 1583.209 kb on - strand at 1583.210 kb on + strand at 1583.210 kb on + strand at 1583.217 kb on + strand at 1583.253 kb on - strand at 1583.254 kb on + strand at 1583.256 kb on + strand at 1583.263 kb on + strand at 1583.347 kb on + strand, within MMP_RS08430 at 1583.621 kb on - strand, within MMP_RS08430 at 1583.622 kb on + strand, within MMP_RS08430 at 1583.624 kb on + strand, within MMP_RS08430 at 1583.624 kb on + strand, within MMP_RS08430 at 1583.628 kb on - strand, within MMP_RS08430 at 1583.646 kb on + strand, within MMP_RS08430 at 1583.646 kb on + strand, within MMP_RS08430 at 1583.967 kb on - strand at 1583.973 kb on - strand at 1583.974 kb on + strand at 1584.034 kb on + strand at 1584.036 kb on - strand at 1584.037 kb on + strand at 1584.037 kb on + strand at 1584.055 kb on - strand at 1584.058 kb on - strand at 1584.060 kb on - strand at 1584.068 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,581,554 + -1.5 1,581,950 + +1.3 1,581,957 - -1.8 1,582,522 - -4.0 1,582,677 + -3.2 1,583,206 + -0.2 1,583,206 + -0.3 1,583,209 - +1.0 1,583,209 - +0.6 1,583,209 - +0.7 1,583,210 + +1.6 1,583,210 + -0.4 1,583,217 + +0.6 1,583,253 - +0.4 1,583,254 + +0.2 1,583,256 + +1.0 1,583,263 + +0.2 1,583,347 + MMP_RS08430 0.17 +0.2 1,583,621 - MMP_RS08430 0.51 +0.2 1,583,622 + MMP_RS08430 0.51 +2.2 1,583,624 + MMP_RS08430 0.52 +0.7 1,583,624 + MMP_RS08430 0.52 -0.6 1,583,628 - MMP_RS08430 0.52 -0.2 1,583,646 + MMP_RS08430 0.54 +1.1 1,583,646 + MMP_RS08430 0.54 -1.2 1,583,967 - +0.4 1,583,973 - +0.4 1,583,974 + +0.7 1,584,034 + +1.5 1,584,036 - +1.2 1,584,037 + +0.1 1,584,037 + +0.1 1,584,055 - -0.0 1,584,058 - +0.3 1,584,060 - +0.0 1,584,068 + -0.4
Or see this region's nucleotide sequence