Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08415 and MMP_RS08420 are separated by 9 nucleotides MMP_RS08420 and MMP_RS08425 are separated by 129 nucleotides
MMP_RS08415: MMP_RS08415 - radical SAM protein, at 1,579,796 to 1,580,659
_RS08415
MMP_RS08420: MMP_RS08420 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,580,669 to 1,581,871
_RS08420
MMP_RS08425: MMP_RS08425 - methionine adenosyltransferase, at 1,582,001 to 1,583,218
_RS08425
Position (kb)
1580
1581
1582 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1579.675 kb on - strand at 1579.676 kb on + strand at 1579.676 kb on + strand at 1579.720 kb on + strand at 1579.720 kb on + strand at 1579.720 kb on + strand at 1579.723 kb on - strand at 1579.724 kb on + strand at 1579.724 kb on + strand at 1579.725 kb on - strand at 1579.725 kb on - strand at 1579.725 kb on - strand at 1579.727 kb on - strand at 1579.727 kb on - strand at 1579.727 kb on - strand at 1579.728 kb on + strand at 1579.728 kb on + strand at 1579.730 kb on - strand at 1579.730 kb on - strand at 1579.733 kb on - strand at 1579.734 kb on + strand at 1579.736 kb on + strand at 1580.269 kb on + strand, within MMP_RS08415 at 1580.322 kb on + strand, within MMP_RS08415 at 1580.485 kb on + strand, within MMP_RS08415 at 1580.774 kb on + strand at 1580.774 kb on + strand at 1580.800 kb on - strand at 1580.959 kb on + strand at 1581.554 kb on + strand at 1581.950 kb on + strand at 1581.957 kb on - strand at 1582.522 kb on - strand at 1582.677 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,579,675 - +0.2 1,579,676 + -0.1 1,579,676 + +0.2 1,579,720 + +0.4 1,579,720 + +0.1 1,579,720 + +0.9 1,579,723 - +0.5 1,579,724 + +1.0 1,579,724 + +1.0 1,579,725 - +0.1 1,579,725 - -0.3 1,579,725 - +1.1 1,579,727 - +0.3 1,579,727 - +0.4 1,579,727 - -0.0 1,579,728 + +1.2 1,579,728 + +0.5 1,579,730 - +0.2 1,579,730 - -0.3 1,579,733 - -0.1 1,579,734 + -0.1 1,579,736 + +0.8 1,580,269 + MMP_RS08415 0.55 -0.2 1,580,322 + MMP_RS08415 0.61 -0.4 1,580,485 + MMP_RS08415 0.80 +0.7 1,580,774 + -3.1 1,580,774 + -2.7 1,580,800 - -1.1 1,580,959 + -3.7 1,581,554 + -1.5 1,581,950 + +1.3 1,581,957 - -1.8 1,582,522 - -4.0 1,582,677 + -3.2
Or see this region's nucleotide sequence