Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08295 and MMP_RS08300 are separated by 69 nucleotides MMP_RS08300 and MMP_RS08305 are separated by 85 nucleotides MMP_RS08305 and MMP_RS08310 are separated by 205 nucleotides MMP_RS08310 and MMP_RS08315 are separated by 10 nucleotides
MMP_RS08295: MMP_RS08295 - histidine--tRNA ligase, at 1,559,794 to 1,561,044
_RS08295
MMP_RS08300: MMP_RS08300 - metal-sulfur cluster assembly factor, at 1,561,114 to 1,561,413
_RS08300
MMP_RS08305: MMP_RS08305 - aspartate--tRNA(Asn) ligase, at 1,561,499 to 1,562,815
_RS08305
MMP_RS08310: MMP_RS08310 - TMEM165/GDT1 family protein, at 1,563,021 to 1,563,284
_RS08310
MMP_RS08315: MMP_RS08315 - S-methyl-5-thioribose-1-phosphate isomerase, at 1,563,295 to 1,564,260
_RS08315
Position (kb)
1561
1562
1563 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1560.746 kb on - strand at 1560.902 kb on + strand at 1561.045 kb on - strand at 1561.087 kb on - strand at 1561.089 kb on - strand at 1561.091 kb on - strand at 1561.092 kb on + strand at 1561.092 kb on + strand at 1561.155 kb on - strand, within MMP_RS08300 at 1561.327 kb on - strand, within MMP_RS08300 at 1561.330 kb on + strand, within MMP_RS08300 at 1561.414 kb on + strand at 1561.437 kb on + strand at 1561.442 kb on + strand at 1561.470 kb on - strand at 1561.474 kb on + strand at 1561.702 kb on + strand at 1562.586 kb on + strand at 1562.586 kb on + strand at 1562.832 kb on + strand at 1562.834 kb on + strand at 1562.895 kb on - strand at 1562.895 kb on - strand at 1562.896 kb on + strand at 1562.896 kb on + strand at 1562.907 kb on - strand at 1562.908 kb on + strand at 1562.908 kb on + strand at 1562.912 kb on - strand at 1562.913 kb on + strand at 1562.913 kb on + strand at 1562.922 kb on + strand at 1562.925 kb on + strand at 1562.951 kb on + strand at 1562.961 kb on + strand at 1562.987 kb on + strand at 1563.003 kb on + strand at 1563.037 kb on + strand at 1563.058 kb on + strand, within MMP_RS08310 at 1563.265 kb on + strand at 1563.300 kb on - strand at 1563.340 kb on + strand at 1563.425 kb on + strand, within MMP_RS08315 at 1563.457 kb on + strand, within MMP_RS08315 at 1563.745 kb on - strand, within MMP_RS08315 at 1563.746 kb on + strand, within MMP_RS08315
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,560,746 - -2.9 1,560,902 + +0.9 1,561,045 - -2.2 1,561,087 - -0.8 1,561,089 - +0.3 1,561,091 - -1.2 1,561,092 + -0.6 1,561,092 + -3.2 1,561,155 - MMP_RS08300 0.14 -2.9 1,561,327 - MMP_RS08300 0.71 -1.7 1,561,330 + MMP_RS08300 0.72 -2.1 1,561,414 + -3.6 1,561,437 + -0.1 1,561,442 + -4.4 1,561,470 - +1.5 1,561,474 + -1.5 1,561,702 + -3.4 1,562,586 + -4.3 1,562,586 + +1.5 1,562,832 + -2.4 1,562,834 + -2.1 1,562,895 - -0.1 1,562,895 - -0.9 1,562,896 + +0.1 1,562,896 + -0.0 1,562,907 - +0.9 1,562,908 + +0.5 1,562,908 + +0.9 1,562,912 - +1.1 1,562,913 + +1.0 1,562,913 + +1.9 1,562,922 + -2.3 1,562,925 + +0.1 1,562,951 + +0.6 1,562,961 + +1.5 1,562,987 + +0.4 1,563,003 + +0.0 1,563,037 + +1.2 1,563,058 + MMP_RS08310 0.14 +1.1 1,563,265 + +0.6 1,563,300 - -0.3 1,563,340 + +1.0 1,563,425 + MMP_RS08315 0.13 +0.0 1,563,457 + MMP_RS08315 0.17 +0.7 1,563,745 - MMP_RS08315 0.47 +0.0 1,563,746 + MMP_RS08315 0.47 +1.6
Or see this region's nucleotide sequence