Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08285 and MMP_RS08290 are separated by 111 nucleotides MMP_RS08290 and MMP_RS08295 are separated by 35 nucleotides MMP_RS08295 and MMP_RS08300 are separated by 69 nucleotides MMP_RS08300 and MMP_RS08305 are separated by 85 nucleotides
MMP_RS08285: MMP_RS08285 - serine/threonine protein kinase, at 1,558,700 to 1,559,377
_RS08285
MMP_RS08290: MMP_RS08290 - DNA-binding protein Alba, at 1,559,489 to 1,559,758
_RS08290
MMP_RS08295: MMP_RS08295 - histidine--tRNA ligase, at 1,559,794 to 1,561,044
_RS08295
MMP_RS08300: MMP_RS08300 - metal-sulfur cluster assembly factor, at 1,561,114 to 1,561,413
_RS08300
MMP_RS08305: MMP_RS08305 - aspartate--tRNA(Asn) ligase, at 1,561,499 to 1,562,815
_RS08305
Position (kb)
1559
1560
1561
1562 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1558.796 kb on - strand, within MMP_RS08285 at 1558.796 kb on - strand, within MMP_RS08285 at 1558.797 kb on + strand, within MMP_RS08285 at 1558.799 kb on + strand, within MMP_RS08285 at 1558.799 kb on + strand, within MMP_RS08285 at 1558.800 kb on - strand, within MMP_RS08285 at 1558.801 kb on + strand, within MMP_RS08285 at 1558.801 kb on + strand, within MMP_RS08285 at 1558.801 kb on + strand, within MMP_RS08285 at 1558.880 kb on + strand, within MMP_RS08285 at 1559.014 kb on - strand, within MMP_RS08285 at 1559.050 kb on + strand, within MMP_RS08285 at 1559.163 kb on + strand, within MMP_RS08285 at 1559.163 kb on + strand, within MMP_RS08285 at 1559.189 kb on + strand, within MMP_RS08285 at 1559.214 kb on - strand, within MMP_RS08285 at 1559.447 kb on + strand at 1559.449 kb on + strand at 1559.451 kb on + strand at 1559.505 kb on + strand at 1559.532 kb on + strand, within MMP_RS08290 at 1559.549 kb on + strand, within MMP_RS08290 at 1559.558 kb on + strand, within MMP_RS08290 at 1559.580 kb on - strand, within MMP_RS08290 at 1559.695 kb on - strand, within MMP_RS08290 at 1559.695 kb on - strand, within MMP_RS08290 at 1559.697 kb on - strand, within MMP_RS08290 at 1559.697 kb on - strand, within MMP_RS08290 at 1559.697 kb on - strand, within MMP_RS08290 at 1559.697 kb on - strand, within MMP_RS08290 at 1559.698 kb on + strand, within MMP_RS08290 at 1559.753 kb on + strand at 1559.756 kb on - strand at 1559.796 kb on + strand at 1559.800 kb on + strand at 1560.293 kb on + strand at 1560.395 kb on + strand at 1560.481 kb on + strand at 1560.746 kb on - strand at 1560.902 kb on + strand at 1561.045 kb on - strand at 1561.087 kb on - strand at 1561.089 kb on - strand at 1561.091 kb on - strand at 1561.092 kb on + strand at 1561.092 kb on + strand at 1561.155 kb on - strand, within MMP_RS08300 at 1561.327 kb on - strand, within MMP_RS08300 at 1561.330 kb on + strand, within MMP_RS08300 at 1561.414 kb on + strand at 1561.437 kb on + strand at 1561.442 kb on + strand at 1561.470 kb on - strand at 1561.474 kb on + strand at 1561.702 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,558,796 - MMP_RS08285 0.14 -0.6 1,558,796 - MMP_RS08285 0.14 +0.9 1,558,797 + MMP_RS08285 0.14 +1.5 1,558,799 + MMP_RS08285 0.15 +1.2 1,558,799 + MMP_RS08285 0.15 +1.2 1,558,800 - MMP_RS08285 0.15 +0.3 1,558,801 + MMP_RS08285 0.15 +0.8 1,558,801 + MMP_RS08285 0.15 +1.2 1,558,801 + MMP_RS08285 0.15 +1.9 1,558,880 + MMP_RS08285 0.27 +0.7 1,559,014 - MMP_RS08285 0.46 -1.3 1,559,050 + MMP_RS08285 0.52 +0.1 1,559,163 + MMP_RS08285 0.68 +0.5 1,559,163 + MMP_RS08285 0.68 +0.4 1,559,189 + MMP_RS08285 0.72 -1.0 1,559,214 - MMP_RS08285 0.76 +0.8 1,559,447 + +0.5 1,559,449 + +1.2 1,559,451 + -0.1 1,559,505 + -0.3 1,559,532 + MMP_RS08290 0.16 -0.9 1,559,549 + MMP_RS08290 0.22 -1.0 1,559,558 + MMP_RS08290 0.26 +0.4 1,559,580 - MMP_RS08290 0.34 -0.9 1,559,695 - MMP_RS08290 0.76 +0.2 1,559,695 - MMP_RS08290 0.76 +0.1 1,559,697 - MMP_RS08290 0.77 -0.0 1,559,697 - MMP_RS08290 0.77 -0.7 1,559,697 - MMP_RS08290 0.77 +0.9 1,559,697 - MMP_RS08290 0.77 -0.2 1,559,698 + MMP_RS08290 0.77 +0.1 1,559,753 + +0.2 1,559,756 - +0.7 1,559,796 + -0.6 1,559,800 + +0.1 1,560,293 + -1.4 1,560,395 + -1.1 1,560,481 + -0.1 1,560,746 - -2.9 1,560,902 + +0.9 1,561,045 - -2.2 1,561,087 - -0.8 1,561,089 - +0.3 1,561,091 - -1.2 1,561,092 + -0.6 1,561,092 + -3.2 1,561,155 - MMP_RS08300 0.14 -2.9 1,561,327 - MMP_RS08300 0.71 -1.7 1,561,330 + MMP_RS08300 0.72 -2.1 1,561,414 + -3.6 1,561,437 + -0.1 1,561,442 + -4.4 1,561,470 - +1.5 1,561,474 + -1.5 1,561,702 + -3.4
Or see this region's nucleotide sequence