Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08250 and MMP_RS08255 are separated by 31 nucleotides MMP_RS08255 and MMP_RS08260 are separated by 10 nucleotides MMP_RS08260 and MMP_RS08265 are separated by 122 nucleotides
MMP_RS08250: MMP_RS08250 - pyruvate kinase, at 1,552,396 to 1,553,739
_RS08250
MMP_RS08255: MMP_RS08255 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 1,553,771 to 1,554,961
_RS08255
MMP_RS08260: MMP_RS08260 - DUF99 family protein, at 1,554,972 to 1,555,523
_RS08260
MMP_RS08265: MMP_RS08265 - rubrerythrin, at 1,555,646 to 1,555,954
_RS08265
Position (kb)
1553
1554
1555 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1552.799 kb on - strand, within MMP_RS08250 at 1552.863 kb on - strand, within MMP_RS08250 at 1552.866 kb on + strand, within MMP_RS08250 at 1552.970 kb on + strand, within MMP_RS08250 at 1553.125 kb on + strand, within MMP_RS08250 at 1553.159 kb on - strand, within MMP_RS08250 at 1553.164 kb on + strand, within MMP_RS08250 at 1553.193 kb on + strand, within MMP_RS08250 at 1553.398 kb on - strand, within MMP_RS08250 at 1553.441 kb on - strand, within MMP_RS08250 at 1553.456 kb on - strand, within MMP_RS08250 at 1553.456 kb on - strand, within MMP_RS08250 at 1553.457 kb on + strand, within MMP_RS08250 at 1553.457 kb on + strand, within MMP_RS08250 at 1553.622 kb on + strand at 1553.653 kb on + strand at 1553.672 kb on + strand at 1553.721 kb on + strand at 1553.736 kb on + strand at 1554.027 kb on + strand at 1554.031 kb on - strand at 1554.154 kb on + strand at 1554.349 kb on - strand at 1554.733 kb on + strand at 1554.817 kb on + strand at 1554.846 kb on + strand at 1554.949 kb on + strand at 1554.949 kb on + strand at 1555.059 kb on - strand, within MMP_RS08260 at 1555.059 kb on - strand, within MMP_RS08260 at 1555.060 kb on + strand, within MMP_RS08260 at 1555.074 kb on + strand, within MMP_RS08260 at 1555.079 kb on + strand, within MMP_RS08260 at 1555.081 kb on - strand, within MMP_RS08260 at 1555.082 kb on + strand, within MMP_RS08260 at 1555.240 kb on + strand, within MMP_RS08260 at 1555.240 kb on + strand, within MMP_RS08260 at 1555.388 kb on - strand, within MMP_RS08260 at 1555.389 kb on + strand, within MMP_RS08260 at 1555.403 kb on - strand, within MMP_RS08260 at 1555.411 kb on - strand, within MMP_RS08260 at 1555.560 kb on + strand at 1555.568 kb on - strand at 1555.569 kb on + strand at 1555.572 kb on - strand at 1555.573 kb on + strand at 1555.573 kb on + strand at 1555.595 kb on + strand at 1555.598 kb on - strand at 1555.621 kb on + strand at 1555.645 kb on - strand at 1555.756 kb on + strand, within MMP_RS08265 at 1555.764 kb on + strand, within MMP_RS08265 at 1555.766 kb on + strand, within MMP_RS08265 at 1555.821 kb on - strand, within MMP_RS08265 at 1555.953 kb on + strand at 1555.958 kb on + strand at 1555.961 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,552,799 - MMP_RS08250 0.30 +0.1 1,552,863 - MMP_RS08250 0.35 -0.2 1,552,866 + MMP_RS08250 0.35 -0.5 1,552,970 + MMP_RS08250 0.43 -0.5 1,553,125 + MMP_RS08250 0.54 +0.7 1,553,159 - MMP_RS08250 0.57 -1.5 1,553,164 + MMP_RS08250 0.57 +0.7 1,553,193 + MMP_RS08250 0.59 -0.7 1,553,398 - MMP_RS08250 0.75 +0.9 1,553,441 - MMP_RS08250 0.78 +0.3 1,553,456 - MMP_RS08250 0.79 +0.9 1,553,456 - MMP_RS08250 0.79 +0.4 1,553,457 + MMP_RS08250 0.79 +0.4 1,553,457 + MMP_RS08250 0.79 +0.6 1,553,622 + +0.9 1,553,653 + +0.4 1,553,672 + +0.6 1,553,721 + -0.1 1,553,736 + +0.2 1,554,027 + -2.8 1,554,031 - -2.2 1,554,154 + -1.7 1,554,349 - -2.7 1,554,733 + -1.5 1,554,817 + -0.7 1,554,846 + -3.7 1,554,949 + +0.0 1,554,949 + +0.7 1,555,059 - MMP_RS08260 0.16 +0.5 1,555,059 - MMP_RS08260 0.16 +0.7 1,555,060 + MMP_RS08260 0.16 +1.3 1,555,074 + MMP_RS08260 0.18 +0.2 1,555,079 + MMP_RS08260 0.19 +0.1 1,555,081 - MMP_RS08260 0.20 +0.6 1,555,082 + MMP_RS08260 0.20 +0.1 1,555,240 + MMP_RS08260 0.49 +1.9 1,555,240 + MMP_RS08260 0.49 +1.2 1,555,388 - MMP_RS08260 0.75 +0.4 1,555,389 + MMP_RS08260 0.76 +0.8 1,555,403 - MMP_RS08260 0.78 +0.9 1,555,411 - MMP_RS08260 0.80 +0.3 1,555,560 + +0.2 1,555,568 - -0.0 1,555,569 + -1.0 1,555,572 - +0.7 1,555,573 + +1.1 1,555,573 + +1.2 1,555,595 + -1.9 1,555,598 - -0.1 1,555,621 + +0.8 1,555,645 - -0.2 1,555,756 + MMP_RS08265 0.36 +0.2 1,555,764 + MMP_RS08265 0.38 +0.5 1,555,766 + MMP_RS08265 0.39 +0.7 1,555,821 - MMP_RS08265 0.57 +0.2 1,555,953 + -0.5 1,555,958 + +1.0 1,555,961 - +0.3
Or see this region's nucleotide sequence