Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08180 and MMP_RS08185 are separated by 38 nucleotides MMP_RS08185 and MMP_RS08190 are separated by 27 nucleotides MMP_RS08190 and MMP_RS08195 are separated by 11 nucleotides
MMP_RS08180: MMP_RS08180 - cobalamin biosynthesis protein, at 1,540,191 to 1,541,162
_RS08180
MMP_RS08185: MMP_RS08185 - tryptophan--tRNA ligase, at 1,541,201 to 1,542,277
_RS08185
MMP_RS08190: MMP_RS08190 - DUF1743 domain-containing protein, at 1,542,305 to 1,543,210
_RS08190
MMP_RS08195: MMP_RS08195 - hypothetical protein, at 1,543,222 to 1,543,764
_RS08195
Position (kb)
1541
1542
1543 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1540.338 kb on + strand at 1540.407 kb on + strand at 1540.505 kb on - strand at 1540.938 kb on - strand at 1540.988 kb on + strand at 1541.147 kb on + strand at 1541.380 kb on + strand at 1541.388 kb on - strand at 1541.390 kb on - strand at 1541.510 kb on - strand at 1541.747 kb on - strand at 1541.895 kb on - strand at 1541.988 kb on - strand at 1541.991 kb on + strand at 1542.451 kb on - strand at 1542.470 kb on + strand at 1542.591 kb on - strand at 1542.649 kb on - strand at 1542.963 kb on - strand at 1543.179 kb on + strand at 1543.196 kb on - strand at 1543.201 kb on + strand at 1543.223 kb on - strand at 1543.223 kb on - strand at 1543.224 kb on + strand at 1543.224 kb on + strand at 1543.224 kb on + strand at 1543.244 kb on - strand at 1543.244 kb on - strand at 1543.245 kb on + strand at 1543.277 kb on + strand, within MMP_RS08195
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,540,338 + -1.3 1,540,407 + -3.0 1,540,505 - -1.2 1,540,938 - -2.8 1,540,988 + -3.2 1,541,147 + -4.2 1,541,380 + -0.7 1,541,388 - -1.9 1,541,390 - -4.0 1,541,510 - -4.7 1,541,747 - -3.2 1,541,895 - -2.9 1,541,988 - -3.8 1,541,991 + -2.1 1,542,451 - -3.0 1,542,470 + -3.9 1,542,591 - -3.2 1,542,649 - -0.4 1,542,963 - -1.7 1,543,179 + -1.2 1,543,196 - -3.1 1,543,201 + -2.1 1,543,223 - -0.2 1,543,223 - -0.9 1,543,224 + -2.8 1,543,224 + -4.2 1,543,224 + -1.1 1,543,244 - +0.4 1,543,244 - +1.3 1,543,245 + -2.1 1,543,277 + MMP_RS08195 0.10 -1.3
Or see this region's nucleotide sequence