Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08170 and MMP_RS08175 are separated by 85 nucleotides MMP_RS08175 and MMP_RS08180 are separated by 0 nucleotides MMP_RS08180 and MMP_RS08185 are separated by 38 nucleotides
MMP_RS08170: MMP_RS08170 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 1,538,219 to 1,539,319
_RS08170
MMP_RS08175: MMP_RS08175 - 7-cyano-7-deazaguanine synthase, at 1,539,405 to 1,540,190
_RS08175
MMP_RS08180: MMP_RS08180 - cobalamin biosynthesis protein, at 1,540,191 to 1,541,162
_RS08180
MMP_RS08185: MMP_RS08185 - tryptophan--tRNA ligase, at 1,541,201 to 1,542,277
_RS08185
Position (kb)
1540
1541
1542 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1539.333 kb on + strand at 1539.352 kb on + strand at 1539.358 kb on + strand at 1539.382 kb on + strand at 1539.465 kb on - strand at 1539.466 kb on + strand at 1539.633 kb on + strand, within MMP_RS08175 at 1539.636 kb on + strand, within MMP_RS08175 at 1539.681 kb on + strand, within MMP_RS08175 at 1539.693 kb on + strand, within MMP_RS08175 at 1539.697 kb on + strand, within MMP_RS08175 at 1539.698 kb on - strand, within MMP_RS08175 at 1539.727 kb on - strand, within MMP_RS08175 at 1539.737 kb on - strand, within MMP_RS08175 at 1539.753 kb on + strand, within MMP_RS08175 at 1539.781 kb on - strand, within MMP_RS08175 at 1539.788 kb on - strand, within MMP_RS08175 at 1539.901 kb on + strand, within MMP_RS08175 at 1540.168 kb on - strand at 1540.169 kb on + strand at 1540.169 kb on + strand at 1540.188 kb on - strand at 1540.192 kb on - strand at 1540.192 kb on - strand at 1540.193 kb on + strand at 1540.197 kb on - strand at 1540.338 kb on + strand at 1540.407 kb on + strand at 1540.505 kb on - strand at 1540.938 kb on - strand at 1540.988 kb on + strand at 1541.147 kb on + strand at 1541.380 kb on + strand at 1541.388 kb on - strand at 1541.390 kb on - strand at 1541.510 kb on - strand at 1541.747 kb on - strand at 1541.895 kb on - strand at 1541.988 kb on - strand at 1541.991 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,539,333 + -0.0 1,539,352 + +0.3 1,539,358 + -0.1 1,539,382 + -0.6 1,539,465 - -0.1 1,539,466 + -0.3 1,539,633 + MMP_RS08175 0.29 +0.5 1,539,636 + MMP_RS08175 0.29 +0.6 1,539,681 + MMP_RS08175 0.35 +0.4 1,539,693 + MMP_RS08175 0.37 +0.3 1,539,697 + MMP_RS08175 0.37 +0.4 1,539,698 - MMP_RS08175 0.37 +0.2 1,539,727 - MMP_RS08175 0.41 -0.1 1,539,737 - MMP_RS08175 0.42 +1.1 1,539,753 + MMP_RS08175 0.44 -0.2 1,539,781 - MMP_RS08175 0.48 -0.9 1,539,788 - MMP_RS08175 0.49 +1.0 1,539,901 + MMP_RS08175 0.63 -2.3 1,540,168 - +1.0 1,540,169 + +1.6 1,540,169 + +1.4 1,540,188 - +0.8 1,540,192 - -0.0 1,540,192 - -1.4 1,540,193 + +1.0 1,540,197 - +0.9 1,540,338 + -1.3 1,540,407 + -3.0 1,540,505 - -1.2 1,540,938 - -2.8 1,540,988 + -3.2 1,541,147 + -4.2 1,541,380 + -0.7 1,541,388 - -1.9 1,541,390 - -4.0 1,541,510 - -4.7 1,541,747 - -3.2 1,541,895 - -2.9 1,541,988 - -3.8 1,541,991 + -2.1
Or see this region's nucleotide sequence