Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08165 and MMP_RS08170 are separated by 143 nucleotides MMP_RS08170 and MMP_RS08175 are separated by 85 nucleotides MMP_RS08175 and MMP_RS08180 are separated by 0 nucleotides
MMP_RS08165: MMP_RS08165 - phosphoglycerate dehydrogenase, at 1,536,504 to 1,538,075
_RS08165
MMP_RS08170: MMP_RS08170 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 1,538,219 to 1,539,319
_RS08170
MMP_RS08175: MMP_RS08175 - 7-cyano-7-deazaguanine synthase, at 1,539,405 to 1,540,190
_RS08175
MMP_RS08180: MMP_RS08180 - cobalamin biosynthesis protein, at 1,540,191 to 1,541,162
_RS08180
Position (kb)
1538
1539
1540 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1537.357 kb on - strand, within MMP_RS08165 at 1537.378 kb on + strand, within MMP_RS08165 at 1538.061 kb on + strand at 1538.077 kb on - strand at 1538.154 kb on + strand at 1538.154 kb on + strand at 1538.162 kb on - strand at 1538.709 kb on + strand at 1539.333 kb on + strand at 1539.352 kb on + strand at 1539.358 kb on + strand at 1539.382 kb on + strand at 1539.465 kb on - strand at 1539.466 kb on + strand at 1539.633 kb on + strand, within MMP_RS08175 at 1539.636 kb on + strand, within MMP_RS08175 at 1539.681 kb on + strand, within MMP_RS08175 at 1539.693 kb on + strand, within MMP_RS08175 at 1539.697 kb on + strand, within MMP_RS08175 at 1539.698 kb on - strand, within MMP_RS08175 at 1539.727 kb on - strand, within MMP_RS08175 at 1539.737 kb on - strand, within MMP_RS08175 at 1539.753 kb on + strand, within MMP_RS08175 at 1539.781 kb on - strand, within MMP_RS08175 at 1539.788 kb on - strand, within MMP_RS08175 at 1539.901 kb on + strand, within MMP_RS08175 at 1540.168 kb on - strand at 1540.169 kb on + strand at 1540.169 kb on + strand at 1540.188 kb on - strand at 1540.192 kb on - strand at 1540.192 kb on - strand at 1540.193 kb on + strand at 1540.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,537,357 - MMP_RS08165 0.54 -1.7 1,537,378 + MMP_RS08165 0.56 -3.2 1,538,061 + -3.8 1,538,077 - -4.2 1,538,154 + -0.4 1,538,154 + +0.3 1,538,162 - +1.5 1,538,709 + -0.1 1,539,333 + -0.0 1,539,352 + +0.3 1,539,358 + -0.1 1,539,382 + -0.6 1,539,465 - -0.1 1,539,466 + -0.3 1,539,633 + MMP_RS08175 0.29 +0.5 1,539,636 + MMP_RS08175 0.29 +0.6 1,539,681 + MMP_RS08175 0.35 +0.4 1,539,693 + MMP_RS08175 0.37 +0.3 1,539,697 + MMP_RS08175 0.37 +0.4 1,539,698 - MMP_RS08175 0.37 +0.2 1,539,727 - MMP_RS08175 0.41 -0.1 1,539,737 - MMP_RS08175 0.42 +1.1 1,539,753 + MMP_RS08175 0.44 -0.2 1,539,781 - MMP_RS08175 0.48 -0.9 1,539,788 - MMP_RS08175 0.49 +1.0 1,539,901 + MMP_RS08175 0.63 -2.3 1,540,168 - +1.0 1,540,169 + +1.6 1,540,169 + +1.4 1,540,188 - +0.8 1,540,192 - -0.0 1,540,192 - -1.4 1,540,193 + +1.0 1,540,197 - +0.9
Or see this region's nucleotide sequence