Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08130 and MMP_RS08135 are separated by 181 nucleotides MMP_RS08135 and MMP_RS08140 are separated by 123 nucleotides MMP_RS08140 and MMP_RS08145 overlap by 4 nucleotides
MMP_RS08130: MMP_RS08130 - IGHMBP2 family helicase, at 1,530,899 to 1,532,800
_RS08130
MMP_RS08135: MMP_RS08135 - arginine decarboxylase pyruvoyl-dependent, at 1,532,982 to 1,533,476
_RS08135
MMP_RS08140: MMP_RS08140 - adenosylmethionine decarboxylase, at 1,533,600 to 1,533,968
_RS08140
MMP_RS08145: MMP_RS08145 - polyamine aminopropyltransferase, at 1,533,965 to 1,534,807
_RS08145
Position (kb)
1532
1533
1534 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1532.119 kb on - strand, within MMP_RS08130 at 1532.289 kb on + strand, within MMP_RS08130 at 1532.297 kb on + strand, within MMP_RS08130 at 1532.520 kb on - strand, within MMP_RS08130 at 1532.520 kb on - strand, within MMP_RS08130 at 1532.524 kb on + strand, within MMP_RS08130 at 1532.549 kb on - strand, within MMP_RS08130 at 1532.677 kb on - strand at 1532.722 kb on + strand at 1532.792 kb on + strand at 1533.132 kb on + strand at 1533.479 kb on - strand at 1533.480 kb on + strand at 1533.481 kb on - strand at 1533.483 kb on - strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.484 kb on + strand at 1533.500 kb on - strand at 1533.501 kb on + strand at 1533.501 kb on + strand at 1533.501 kb on + strand at 1533.506 kb on + strand at 1533.517 kb on - strand at 1533.518 kb on + strand at 1533.518 kb on + strand at 1533.839 kb on + strand at 1533.839 kb on + strand at 1534.035 kb on - strand at 1534.040 kb on - strand at 1534.148 kb on + strand at 1534.152 kb on + strand at 1534.306 kb on + strand at 1534.391 kb on - strand at 1534.392 kb on + strand at 1534.423 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,532,119 - MMP_RS08130 0.64 -0.2 1,532,289 + MMP_RS08130 0.73 -1.1 1,532,297 + MMP_RS08130 0.74 +0.1 1,532,520 - MMP_RS08130 0.85 +0.3 1,532,520 - MMP_RS08130 0.85 +0.5 1,532,524 + MMP_RS08130 0.85 +1.1 1,532,549 - MMP_RS08130 0.87 +0.6 1,532,677 - +0.9 1,532,722 + +0.7 1,532,792 + +1.1 1,533,132 + -4.1 1,533,479 - +2.0 1,533,480 + +0.7 1,533,481 - +0.4 1,533,483 - +0.6 1,533,484 + +1.1 1,533,484 + +2.1 1,533,484 + -0.2 1,533,484 + +0.3 1,533,484 + +0.6 1,533,500 - +1.4 1,533,501 + +0.5 1,533,501 + +1.3 1,533,501 + +1.1 1,533,506 + +0.5 1,533,517 - -0.6 1,533,518 + +0.8 1,533,518 + +1.4 1,533,839 + -2.7 1,533,839 + -0.5 1,534,035 - -0.4 1,534,040 - -1.5 1,534,148 + -2.5 1,534,152 + -1.7 1,534,306 + -0.6 1,534,391 - -1.4 1,534,392 + -0.2 1,534,423 + -0.6
Or see this region's nucleotide sequence