Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08105 and MMP_RS09035 are separated by 186 nucleotides MMP_RS09035 and MMP_RS08110 are separated by 15 nucleotides MMP_RS08110 and MMP_RS08115 are separated by 130 nucleotides MMP_RS08115 and MMP_RS08120 are separated by 135 nucleotides
MMP_RS08105: MMP_RS08105 - 6-carboxyhexanoate--CoA ligase, at 1,525,190 to 1,525,924
_RS08105
MMP_RS09035: MMP_RS09035 - hypothetical protein, at 1,526,111 to 1,526,266
_RS09035
MMP_RS08110: MMP_RS08110 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,526,282 to 1,527,904
_RS08110
MMP_RS08115: MMP_RS08115 - nicotinamide-nucleotide adenylyltransferase, at 1,528,035 to 1,528,550
_RS08115
MMP_RS08120: MMP_RS08120 - 30S ribosomal protein S15, at 1,528,686 to 1,529,141
_RS08120
Position (kb)
1526
1527
1528 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1525.392 kb on + strand, within MMP_RS08105 at 1525.437 kb on + strand, within MMP_RS08105 at 1525.544 kb on - strand, within MMP_RS08105 at 1525.565 kb on + strand, within MMP_RS08105 at 1525.565 kb on + strand, within MMP_RS08105 at 1525.585 kb on - strand, within MMP_RS08105 at 1525.602 kb on + strand, within MMP_RS08105 at 1525.621 kb on - strand, within MMP_RS08105 at 1525.673 kb on - strand, within MMP_RS08105 at 1525.722 kb on + strand, within MMP_RS08105 at 1526.061 kb on + strand at 1526.064 kb on - strand at 1526.066 kb on - strand at 1526.067 kb on + strand at 1526.069 kb on + strand at 1526.069 kb on + strand at 1526.085 kb on + strand at 1526.119 kb on - strand at 1526.129 kb on + strand, within MMP_RS09035 at 1526.235 kb on - strand, within MMP_RS09035 at 1526.236 kb on + strand, within MMP_RS09035 at 1526.242 kb on - strand, within MMP_RS09035 at 1526.243 kb on + strand, within MMP_RS09035 at 1526.254 kb on + strand at 1526.269 kb on - strand at 1526.283 kb on - strand at 1526.337 kb on - strand at 1526.338 kb on + strand at 1526.341 kb on + strand at 1526.563 kb on - strand at 1526.571 kb on + strand at 1526.882 kb on + strand at 1527.074 kb on - strand at 1527.182 kb on + strand at 1527.550 kb on + strand at 1527.875 kb on + strand at 1527.928 kb on + strand at 1527.937 kb on - strand at 1527.937 kb on - strand at 1527.938 kb on + strand at 1527.940 kb on + strand at 1527.940 kb on + strand at 1527.952 kb on - strand at 1527.955 kb on + strand at 1527.965 kb on + strand at 1527.997 kb on - strand at 1528.002 kb on + strand at 1528.002 kb on + strand at 1528.035 kb on + strand at 1528.084 kb on - strand at 1528.163 kb on + strand at 1528.229 kb on + strand at 1528.259 kb on + strand at 1528.390 kb on + strand at 1528.390 kb on + strand at 1528.522 kb on - strand at 1528.548 kb on - strand at 1528.562 kb on - strand at 1528.595 kb on - strand at 1528.598 kb on + strand at 1528.599 kb on - strand at 1528.601 kb on - strand at 1528.601 kb on - strand at 1528.681 kb on - strand at 1528.853 kb on - strand at 1528.857 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,525,392 + MMP_RS08105 0.27 -1.2 1,525,437 + MMP_RS08105 0.34 -0.1 1,525,544 - MMP_RS08105 0.48 -0.4 1,525,565 + MMP_RS08105 0.51 -0.4 1,525,565 + MMP_RS08105 0.51 -1.7 1,525,585 - MMP_RS08105 0.54 +0.1 1,525,602 + MMP_RS08105 0.56 +0.1 1,525,621 - MMP_RS08105 0.59 +1.6 1,525,673 - MMP_RS08105 0.66 -0.4 1,525,722 + MMP_RS08105 0.72 -1.1 1,526,061 + -0.5 1,526,064 - +0.5 1,526,066 - -0.6 1,526,067 + +0.2 1,526,069 + -0.1 1,526,069 + -0.6 1,526,085 + -0.2 1,526,119 - +0.1 1,526,129 + MMP_RS09035 0.12 -1.3 1,526,235 - MMP_RS09035 0.79 -1.3 1,526,236 + MMP_RS09035 0.80 +0.4 1,526,242 - MMP_RS09035 0.84 +0.2 1,526,243 + MMP_RS09035 0.85 +0.0 1,526,254 + +0.3 1,526,269 - +0.2 1,526,283 - +0.3 1,526,337 - -1.6 1,526,338 + -1.7 1,526,341 + -1.0 1,526,563 - -3.4 1,526,571 + -1.5 1,526,882 + -1.5 1,527,074 - +0.3 1,527,182 + -0.8 1,527,550 + -1.7 1,527,875 + -2.2 1,527,928 + -3.1 1,527,937 - -2.2 1,527,937 - +0.4 1,527,938 + -3.1 1,527,940 + -3.1 1,527,940 + -4.1 1,527,952 - -1.0 1,527,955 + -0.3 1,527,965 + -3.0 1,527,997 - -4.5 1,528,002 + -2.1 1,528,002 + -1.9 1,528,035 + -2.6 1,528,084 - -2.5 1,528,163 + -3.5 1,528,229 + -1.7 1,528,259 + -0.8 1,528,390 + -2.7 1,528,390 + -2.9 1,528,522 - -3.8 1,528,548 - -0.1 1,528,562 - -0.3 1,528,595 - -0.6 1,528,598 + -1.8 1,528,599 - -1.1 1,528,601 - -5.3 1,528,601 - -3.7 1,528,681 - -2.7 1,528,853 - -1.7 1,528,857 - -2.7
Or see this region's nucleotide sequence