Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07920 and MMP_RS07925 are separated by 60 nucleotides MMP_RS07925 and MMP_RS07930 are separated by 268 nucleotides MMP_RS07930 and MMP_RS07935 are separated by 30 nucleotides MMP_RS07935 and MMP_RS07940 are separated by 21 nucleotides
MMP_RS07920: MMP_RS07920 - tRNA-Ile, at 1,497,882 to 1,497,956
_RS07920
MMP_RS07925: MMP_RS07925 - haloacid dehalogenase, at 1,498,017 to 1,498,607
_RS07925
MMP_RS07930: MMP_RS07930 - 50S ribosomal protein L3, at 1,498,876 to 1,499,880
_RS07930
MMP_RS07935: MMP_RS07935 - 50S ribosomal protein L4, at 1,499,911 to 1,500,669
_RS07935
MMP_RS07940: MMP_RS07940 - 50S ribosomal protein L23, at 1,500,691 to 1,500,951
_RS07940
Position (kb)
1498
1499
1500 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1498.074 kb on - strand at 1498.075 kb on + strand at 1498.075 kb on + strand at 1498.141 kb on + strand, within MMP_RS07925 at 1498.188 kb on + strand, within MMP_RS07925 at 1498.299 kb on - strand, within MMP_RS07925 at 1498.300 kb on + strand, within MMP_RS07925 at 1498.355 kb on + strand, within MMP_RS07925 at 1498.639 kb on - strand at 1498.669 kb on + strand at 1498.704 kb on - strand at 1499.007 kb on + strand at 1499.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,498,074 - +0.6 1,498,075 + +0.7 1,498,075 + +0.3 1,498,141 + MMP_RS07925 0.21 +0.4 1,498,188 + MMP_RS07925 0.29 -0.4 1,498,299 - MMP_RS07925 0.48 +1.2 1,498,300 + MMP_RS07925 0.48 +0.3 1,498,355 + MMP_RS07925 0.57 -0.0 1,498,639 - -0.0 1,498,669 + +1.8 1,498,704 - -1.9 1,499,007 + -0.5 1,499,096 - -0.1
Or see this region's nucleotide sequence