Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07480 and MMP_RS07485 are separated by 21 nucleotides MMP_RS07485 and MMP_RS07490 are separated by 9 nucleotides MMP_RS07490 and MMP_RS07495 are separated by 19 nucleotides MMP_RS07495 and MMP_RS07500 are separated by 8 nucleotides
MMP_RS07480: MMP_RS07480 - EhaG family protein, at 1,420,103 to 1,420,789
_RS07480
MMP_RS07485: MMP_RS07485 - membrane protein, at 1,420,811 to 1,421,473
_RS07485
MMP_RS07490: MMP_RS07490 - hypothetical protein, at 1,421,483 to 1,421,692
_RS07490
MMP_RS07495: MMP_RS07495 - NADH-quinone oxidoreductase subunit H, at 1,421,712 to 1,422,572
_RS07495
MMP_RS07500: MMP_RS07500 - hypothetical protein, at 1,422,581 to 1,422,832
_RS07500
Position (kb)
1421
1422 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1420.808 kb on + strand at 1420.896 kb on - strand at 1421.043 kb on - strand at 1421.044 kb on + strand at 1421.073 kb on - strand at 1421.399 kb on - strand at 1421.542 kb on + strand at 1421.709 kb on + strand at 1421.711 kb on - strand at 1421.730 kb on - strand at 1421.898 kb on - strand at 1421.898 kb on - strand at 1421.981 kb on + strand at 1422.285 kb on + strand at 1422.302 kb on - strand at 1422.367 kb on - strand at 1422.368 kb on + strand at 1422.556 kb on + strand at 1422.591 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,420,808 + -4.3 1,420,896 - -2.4 1,421,043 - -4.5 1,421,044 + -2.3 1,421,073 - -2.1 1,421,399 - -1.7 1,421,542 + -4.5 1,421,709 + -3.3 1,421,711 - -4.5 1,421,730 - -3.3 1,421,898 - -4.5 1,421,898 - -4.5 1,421,981 + -2.2 1,422,285 + -3.5 1,422,302 - -4.5 1,422,367 - -2.1 1,422,368 + -2.1 1,422,556 + -4.6 1,422,591 + -3.9
Or see this region's nucleotide sequence