Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07455 and MMP_RS07460 are separated by 0 nucleotides MMP_RS07460 and MMP_RS07465 are separated by 5 nucleotides MMP_RS07465 and MMP_RS07470 are separated by 8 nucleotides MMP_RS07470 and MMP_RS07475 are separated by 1 nucleotides MMP_RS07475 and MMP_RS07480 overlap by 1 nucleotides MMP_RS07480 and MMP_RS07485 are separated by 21 nucleotides
MMP_RS07455: MMP_RS07455 - hypothetical protein, at 1,418,323 to 1,418,814
_RS07455
MMP_RS07460: MMP_RS07460 - DUF2109 domain-containing protein, at 1,418,815 to 1,419,081
_RS07460
MMP_RS07465: MMP_RS07465 - DUF2108 domain-containing protein, at 1,419,087 to 1,419,365
_RS07465
MMP_RS07470: MMP_RS07470 - EhaE family protein, at 1,419,374 to 1,419,628
_RS07470
MMP_RS07475: MMP_RS07475 - EhaF family protein, at 1,419,630 to 1,420,103
_RS07475
MMP_RS07480: MMP_RS07480 - EhaG family protein, at 1,420,103 to 1,420,789
_RS07480
MMP_RS07485: MMP_RS07485 - membrane protein, at 1,420,811 to 1,421,473
_RS07485
Position (kb)
1419
1420
1421 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1418.868 kb on + strand at 1418.953 kb on - strand at 1419.137 kb on + strand at 1419.172 kb on - strand at 1419.309 kb on - strand at 1419.309 kb on - strand at 1419.364 kb on + strand at 1419.517 kb on + strand at 1419.946 kb on + strand at 1420.228 kb on + strand at 1420.245 kb on - strand at 1420.246 kb on + strand at 1420.455 kb on - strand at 1420.456 kb on + strand at 1420.456 kb on + strand at 1420.808 kb on + strand at 1420.896 kb on - strand at 1421.043 kb on - strand at 1421.044 kb on + strand at 1421.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,418,868 + -3.1 1,418,953 - -5.3 1,419,137 + -4.0 1,419,172 - -2.3 1,419,309 - -4.3 1,419,309 - -4.7 1,419,364 + -3.8 1,419,517 + -3.6 1,419,946 + -4.5 1,420,228 + -2.1 1,420,245 - -2.5 1,420,246 + -3.0 1,420,455 - -2.3 1,420,456 + -3.7 1,420,456 + -2.9 1,420,808 + -4.3 1,420,896 - -2.4 1,421,043 - -4.5 1,421,044 + -2.3 1,421,073 - -2.1
Or see this region's nucleotide sequence