Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07365 and MMP_RS07370 are separated by 84 nucleotides MMP_RS07370 and MMP_RS07375 are separated by 109 nucleotides MMP_RS07375 and MMP_RS07380 are separated by 113 nucleotides
MMP_RS07365: MMP_RS07365 - 2-phosphoglycerate kinase, at 1,402,976 to 1,403,914
_RS07365
MMP_RS07370: MMP_RS07370 - adenylosuccinate synthetase, at 1,403,999 to 1,405,015
_RS07370
MMP_RS07375: MMP_RS07375 - 50S ribosomal protein L11, at 1,405,125 to 1,405,604
_RS07375
MMP_RS07380: MMP_RS07380 - transcription elongation factor Spt5, at 1,405,718 to 1,406,158
_RS07380
Position (kb)
1403
1404
1405
1406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1403.012 kb on - strand at 1403.032 kb on - strand at 1403.083 kb on - strand, within MMP_RS07365 at 1403.119 kb on - strand, within MMP_RS07365 at 1403.119 kb on - strand, within MMP_RS07365 at 1403.120 kb on + strand, within MMP_RS07365 at 1403.120 kb on + strand, within MMP_RS07365 at 1403.121 kb on - strand, within MMP_RS07365 at 1403.122 kb on + strand, within MMP_RS07365 at 1403.122 kb on + strand, within MMP_RS07365 at 1403.122 kb on + strand, within MMP_RS07365 at 1403.138 kb on - strand, within MMP_RS07365 at 1403.183 kb on - strand, within MMP_RS07365 at 1403.201 kb on + strand, within MMP_RS07365 at 1403.231 kb on - strand, within MMP_RS07365 at 1403.285 kb on - strand, within MMP_RS07365 at 1403.298 kb on + strand, within MMP_RS07365 at 1403.298 kb on + strand, within MMP_RS07365 at 1403.315 kb on - strand, within MMP_RS07365 at 1403.344 kb on - strand, within MMP_RS07365 at 1403.344 kb on - strand, within MMP_RS07365 at 1403.345 kb on + strand, within MMP_RS07365 at 1403.345 kb on + strand, within MMP_RS07365 at 1403.349 kb on + strand, within MMP_RS07365 at 1403.349 kb on + strand, within MMP_RS07365 at 1403.351 kb on + strand, within MMP_RS07365 at 1403.446 kb on - strand, within MMP_RS07365 at 1403.464 kb on - strand, within MMP_RS07365 at 1403.466 kb on - strand, within MMP_RS07365 at 1403.492 kb on - strand, within MMP_RS07365 at 1403.507 kb on + strand, within MMP_RS07365 at 1403.626 kb on - strand, within MMP_RS07365 at 1403.632 kb on - strand, within MMP_RS07365 at 1403.633 kb on + strand, within MMP_RS07365 at 1403.709 kb on - strand, within MMP_RS07365 at 1403.751 kb on + strand, within MMP_RS07365 at 1403.766 kb on + strand, within MMP_RS07365 at 1403.918 kb on + strand at 1403.953 kb on + strand at 1403.955 kb on - strand at 1403.955 kb on - strand at 1403.955 kb on - strand at 1403.955 kb on - strand at 1403.956 kb on + strand at 1403.957 kb on - strand at 1403.957 kb on - strand at 1403.957 kb on - strand at 1403.957 kb on - strand at 1403.957 kb on - strand at 1403.958 kb on + strand at 1403.958 kb on + strand at 1403.958 kb on + strand at 1403.960 kb on + strand at 1403.962 kb on - strand at 1403.962 kb on - strand at 1403.963 kb on + strand at 1403.963 kb on + strand at 1403.981 kb on - strand at 1404.176 kb on - strand at 1404.177 kb on + strand at 1404.437 kb on + strand at 1404.887 kb on + strand at 1404.957 kb on + strand at 1405.255 kb on - strand at 1405.646 kb on + strand at 1405.673 kb on + strand at 1405.720 kb on + strand at 1405.771 kb on - strand, within MMP_RS07380 at 1405.790 kb on - strand, within MMP_RS07380 at 1405.831 kb on - strand, within MMP_RS07380 at 1405.958 kb on - strand, within MMP_RS07380 at 1405.970 kb on - strand, within MMP_RS07380
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,403,012 - -1.0 1,403,032 - -1.2 1,403,083 - MMP_RS07365 0.11 -3.6 1,403,119 - MMP_RS07365 0.15 -2.5 1,403,119 - MMP_RS07365 0.15 -4.4 1,403,120 + MMP_RS07365 0.15 -3.2 1,403,120 + MMP_RS07365 0.15 -0.6 1,403,121 - MMP_RS07365 0.15 -3.8 1,403,122 + MMP_RS07365 0.16 -4.0 1,403,122 + MMP_RS07365 0.16 -4.4 1,403,122 + MMP_RS07365 0.16 -1.7 1,403,138 - MMP_RS07365 0.17 -2.6 1,403,183 - MMP_RS07365 0.22 -2.0 1,403,201 + MMP_RS07365 0.24 -3.6 1,403,231 - MMP_RS07365 0.27 -2.4 1,403,285 - MMP_RS07365 0.33 -3.8 1,403,298 + MMP_RS07365 0.34 -3.9 1,403,298 + MMP_RS07365 0.34 -3.8 1,403,315 - MMP_RS07365 0.36 -3.9 1,403,344 - MMP_RS07365 0.39 -3.7 1,403,344 - MMP_RS07365 0.39 -2.9 1,403,345 + MMP_RS07365 0.39 -2.9 1,403,345 + MMP_RS07365 0.39 -2.8 1,403,349 + MMP_RS07365 0.40 -2.7 1,403,349 + MMP_RS07365 0.40 -3.8 1,403,351 + MMP_RS07365 0.40 -3.9 1,403,446 - MMP_RS07365 0.50 -2.4 1,403,464 - MMP_RS07365 0.52 -3.4 1,403,466 - MMP_RS07365 0.52 -3.4 1,403,492 - MMP_RS07365 0.55 -3.8 1,403,507 + MMP_RS07365 0.57 -2.4 1,403,626 - MMP_RS07365 0.69 -3.4 1,403,632 - MMP_RS07365 0.70 -1.5 1,403,633 + MMP_RS07365 0.70 -4.3 1,403,709 - MMP_RS07365 0.78 -3.6 1,403,751 + MMP_RS07365 0.83 -4.4 1,403,766 + MMP_RS07365 0.84 -4.2 1,403,918 + -2.5 1,403,953 + -1.2 1,403,955 - -1.2 1,403,955 - +0.1 1,403,955 - +0.2 1,403,955 - -2.5 1,403,956 + -3.1 1,403,957 - -1.8 1,403,957 - -0.3 1,403,957 - -2.8 1,403,957 - +0.4 1,403,957 - -1.1 1,403,958 + -1.6 1,403,958 + -0.8 1,403,958 + -3.0 1,403,960 + -3.1 1,403,962 - +0.5 1,403,962 - -1.1 1,403,963 + -1.9 1,403,963 + -1.5 1,403,981 - -0.6 1,404,176 - -3.3 1,404,177 + -1.4 1,404,437 + -1.5 1,404,887 + -2.8 1,404,957 + -1.2 1,405,255 - -4.0 1,405,646 + -2.3 1,405,673 + +1.3 1,405,720 + -0.2 1,405,771 - MMP_RS07380 0.12 -0.8 1,405,790 - MMP_RS07380 0.16 -4.7 1,405,831 - MMP_RS07380 0.26 -2.5 1,405,958 - MMP_RS07380 0.54 -1.6 1,405,970 - MMP_RS07380 0.57 -1.1
Or see this region's nucleotide sequence