Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07345 and MMP_RS07350 are separated by 72 nucleotides MMP_RS07350 and MMP_RS07355 are separated by 54 nucleotides MMP_RS07355 and MMP_RS07360 are separated by 71 nucleotides MMP_RS07360 and MMP_RS07365 are separated by 21 nucleotides
MMP_RS07345: MMP_RS07345 - glycine synthesis protein GlyXL (from data) , at 1,399,464 to 1,400,840
_RS07345
MMP_RS07350: MMP_RS07350 - preprotein translocase subunit Sec61beta, at 1,400,913 to 1,401,074
_RS07350
MMP_RS07355: MMP_RS07355 - transcription factor S, at 1,401,129 to 1,401,446
_RS07355
MMP_RS07360: MMP_RS07360 - TrkH family potassium uptake protein, at 1,401,518 to 1,402,954
_RS07360
MMP_RS07365: MMP_RS07365 - 2-phosphoglycerate kinase, at 1,402,976 to 1,403,914
_RS07365
Position (kb)
1401
1402
1403 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1400.673 kb on + strand, within MMP_RS07345 at 1400.889 kb on + strand at 1400.890 kb on - strand at 1400.891 kb on + strand at 1400.965 kb on + strand, within MMP_RS07350 at 1400.965 kb on + strand, within MMP_RS07350 at 1400.989 kb on - strand, within MMP_RS07350 at 1401.112 kb on + strand at 1401.127 kb on + strand at 1401.131 kb on + strand at 1401.131 kb on + strand at 1401.137 kb on + strand at 1401.150 kb on + strand at 1401.255 kb on - strand, within MMP_RS07355 at 1401.417 kb on + strand at 1401.479 kb on + strand at 1401.481 kb on + strand at 1401.481 kb on + strand at 1401.512 kb on - strand at 1401.574 kb on + strand at 1401.796 kb on + strand at 1401.811 kb on - strand at 1402.015 kb on - strand at 1402.032 kb on + strand at 1402.139 kb on - strand at 1402.234 kb on + strand at 1402.292 kb on - strand at 1402.293 kb on + strand at 1402.324 kb on - strand at 1402.361 kb on - strand at 1402.362 kb on + strand at 1402.393 kb on + strand at 1402.476 kb on - strand at 1402.681 kb on + strand at 1403.012 kb on - strand at 1403.032 kb on - strand at 1403.083 kb on - strand, within MMP_RS07365 at 1403.119 kb on - strand, within MMP_RS07365 at 1403.119 kb on - strand, within MMP_RS07365 at 1403.120 kb on + strand, within MMP_RS07365 at 1403.120 kb on + strand, within MMP_RS07365 at 1403.121 kb on - strand, within MMP_RS07365 at 1403.122 kb on + strand, within MMP_RS07365 at 1403.122 kb on + strand, within MMP_RS07365 at 1403.122 kb on + strand, within MMP_RS07365 at 1403.138 kb on - strand, within MMP_RS07365 at 1403.183 kb on - strand, within MMP_RS07365 at 1403.201 kb on + strand, within MMP_RS07365 at 1403.231 kb on - strand, within MMP_RS07365 at 1403.285 kb on - strand, within MMP_RS07365 at 1403.298 kb on + strand, within MMP_RS07365 at 1403.298 kb on + strand, within MMP_RS07365 at 1403.315 kb on - strand, within MMP_RS07365 at 1403.344 kb on - strand, within MMP_RS07365 at 1403.344 kb on - strand, within MMP_RS07365 at 1403.345 kb on + strand, within MMP_RS07365 at 1403.345 kb on + strand, within MMP_RS07365 at 1403.349 kb on + strand, within MMP_RS07365 at 1403.349 kb on + strand, within MMP_RS07365 at 1403.351 kb on + strand, within MMP_RS07365 at 1403.446 kb on - strand, within MMP_RS07365 at 1403.464 kb on - strand, within MMP_RS07365 at 1403.466 kb on - strand, within MMP_RS07365 at 1403.492 kb on - strand, within MMP_RS07365 at 1403.507 kb on + strand, within MMP_RS07365 at 1403.626 kb on - strand, within MMP_RS07365 at 1403.632 kb on - strand, within MMP_RS07365 at 1403.633 kb on + strand, within MMP_RS07365 at 1403.709 kb on - strand, within MMP_RS07365 at 1403.751 kb on + strand, within MMP_RS07365 at 1403.766 kb on + strand, within MMP_RS07365 at 1403.918 kb on + strand at 1403.953 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,400,673 + MMP_RS07345 0.88 -3.0 1,400,889 + -3.8 1,400,890 - +0.8 1,400,891 + -1.2 1,400,965 + MMP_RS07350 0.32 -0.5 1,400,965 + MMP_RS07350 0.32 +0.9 1,400,989 - MMP_RS07350 0.47 -0.4 1,401,112 + +0.4 1,401,127 + -0.2 1,401,131 + +1.5 1,401,131 + +0.1 1,401,137 + +0.3 1,401,150 + -4.1 1,401,255 - MMP_RS07355 0.40 -1.9 1,401,417 + -2.4 1,401,479 + -3.1 1,401,481 + -3.8 1,401,481 + -2.8 1,401,512 - -1.4 1,401,574 + -2.4 1,401,796 + -2.3 1,401,811 - -1.0 1,402,015 - -1.6 1,402,032 + -2.3 1,402,139 - -3.2 1,402,234 + -1.7 1,402,292 - -3.8 1,402,293 + -1.7 1,402,324 - -1.4 1,402,361 - -1.8 1,402,362 + -3.8 1,402,393 + -1.6 1,402,476 - -4.0 1,402,681 + -2.9 1,403,012 - -1.0 1,403,032 - -1.2 1,403,083 - MMP_RS07365 0.11 -3.6 1,403,119 - MMP_RS07365 0.15 -2.5 1,403,119 - MMP_RS07365 0.15 -4.4 1,403,120 + MMP_RS07365 0.15 -3.2 1,403,120 + MMP_RS07365 0.15 -0.6 1,403,121 - MMP_RS07365 0.15 -3.8 1,403,122 + MMP_RS07365 0.16 -4.0 1,403,122 + MMP_RS07365 0.16 -4.4 1,403,122 + MMP_RS07365 0.16 -1.7 1,403,138 - MMP_RS07365 0.17 -2.6 1,403,183 - MMP_RS07365 0.22 -2.0 1,403,201 + MMP_RS07365 0.24 -3.6 1,403,231 - MMP_RS07365 0.27 -2.4 1,403,285 - MMP_RS07365 0.33 -3.8 1,403,298 + MMP_RS07365 0.34 -3.9 1,403,298 + MMP_RS07365 0.34 -3.8 1,403,315 - MMP_RS07365 0.36 -3.9 1,403,344 - MMP_RS07365 0.39 -3.7 1,403,344 - MMP_RS07365 0.39 -2.9 1,403,345 + MMP_RS07365 0.39 -2.9 1,403,345 + MMP_RS07365 0.39 -2.8 1,403,349 + MMP_RS07365 0.40 -2.7 1,403,349 + MMP_RS07365 0.40 -3.8 1,403,351 + MMP_RS07365 0.40 -3.9 1,403,446 - MMP_RS07365 0.50 -2.4 1,403,464 - MMP_RS07365 0.52 -3.4 1,403,466 - MMP_RS07365 0.52 -3.4 1,403,492 - MMP_RS07365 0.55 -3.8 1,403,507 + MMP_RS07365 0.57 -2.4 1,403,626 - MMP_RS07365 0.69 -3.4 1,403,632 - MMP_RS07365 0.70 -1.5 1,403,633 + MMP_RS07365 0.70 -4.3 1,403,709 - MMP_RS07365 0.78 -3.6 1,403,751 + MMP_RS07365 0.83 -4.4 1,403,766 + MMP_RS07365 0.84 -4.2 1,403,918 + -2.5 1,403,953 + -1.2
Or see this region's nucleotide sequence