Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07320 and MMP_RS07325 are separated by 39 nucleotides MMP_RS07325 and MMP_RS07330 are separated by 96 nucleotides
MMP_RS07320: MMP_RS07320 - preprotein translocase subunit SecY, at 1,393,242 to 1,394,570
_RS07320
MMP_RS07325: MMP_RS07325 - glycosyl transferase, at 1,394,610 to 1,395,509
_RS07325
MMP_RS07330: MMP_RS07330 - membrane protein, at 1,395,606 to 1,398,164
_RS07330
Position (kb)
1394
1395
1396 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1393.915 kb on - strand at 1394.152 kb on + strand at 1394.234 kb on + strand at 1394.502 kb on - strand at 1394.582 kb on - strand at 1394.644 kb on + strand at 1394.692 kb on + strand at 1394.702 kb on + strand at 1394.799 kb on - strand at 1394.985 kb on - strand at 1394.986 kb on + strand at 1395.039 kb on + strand at 1395.421 kb on - strand at 1395.490 kb on + strand at 1395.523 kb on + strand at 1395.523 kb on + strand at 1395.523 kb on + strand at 1395.528 kb on + strand at 1395.554 kb on - strand at 1395.555 kb on + strand at 1395.558 kb on - strand at 1395.558 kb on - strand at 1395.559 kb on + strand at 1395.560 kb on - strand at 1395.561 kb on + strand at 1395.561 kb on + strand at 1395.561 kb on + strand at 1395.561 kb on + strand at 1395.563 kb on + strand at 1395.564 kb on - strand at 1395.564 kb on - strand at 1395.564 kb on - strand at 1395.565 kb on + strand at 1395.565 kb on + strand at 1395.565 kb on + strand at 1395.565 kb on + strand at 1395.758 kb on - strand at 1395.758 kb on - strand at 1395.787 kb on + strand at 1395.787 kb on + strand at 1395.960 kb on + strand, within MMP_RS07330 at 1396.053 kb on + strand, within MMP_RS07330 at 1396.106 kb on - strand, within MMP_RS07330 at 1396.106 kb on - strand, within MMP_RS07330 at 1396.107 kb on + strand, within MMP_RS07330 at 1396.107 kb on + strand, within MMP_RS07330 at 1396.157 kb on + strand, within MMP_RS07330 at 1396.158 kb on - strand, within MMP_RS07330 at 1396.158 kb on - strand, within MMP_RS07330 at 1396.159 kb on + strand, within MMP_RS07330 at 1396.159 kb on + strand, within MMP_RS07330 at 1396.160 kb on - strand, within MMP_RS07330 at 1396.201 kb on - strand, within MMP_RS07330 at 1396.213 kb on - strand, within MMP_RS07330 at 1396.222 kb on - strand, within MMP_RS07330 at 1396.311 kb on - strand, within MMP_RS07330 at 1396.370 kb on + strand, within MMP_RS07330 at 1396.372 kb on - strand, within MMP_RS07330 at 1396.372 kb on - strand, within MMP_RS07330 at 1396.373 kb on + strand, within MMP_RS07330 at 1396.374 kb on - strand, within MMP_RS07330 at 1396.374 kb on - strand, within MMP_RS07330 at 1396.383 kb on - strand, within MMP_RS07330 at 1396.383 kb on - strand, within MMP_RS07330 at 1396.384 kb on + strand, within MMP_RS07330 at 1396.394 kb on + strand, within MMP_RS07330 at 1396.394 kb on + strand, within MMP_RS07330 at 1396.394 kb on + strand, within MMP_RS07330 at 1396.439 kb on - strand, within MMP_RS07330 at 1396.440 kb on + strand, within MMP_RS07330 at 1396.440 kb on + strand, within MMP_RS07330 at 1396.445 kb on + strand, within MMP_RS07330 at 1396.445 kb on + strand, within MMP_RS07330 at 1396.445 kb on + strand, within MMP_RS07330 at 1396.445 kb on + strand, within MMP_RS07330 at 1396.445 kb on + strand, within MMP_RS07330 at 1396.494 kb on + strand, within MMP_RS07330 at 1396.496 kb on - strand, within MMP_RS07330
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,393,915 - -1.9 1,394,152 + -2.0 1,394,234 + -2.2 1,394,502 - -0.6 1,394,582 - -1.5 1,394,644 + -1.7 1,394,692 + -0.6 1,394,702 + -1.4 1,394,799 - -1.5 1,394,985 - -2.9 1,394,986 + -1.5 1,395,039 + -0.3 1,395,421 - -0.7 1,395,490 + -0.0 1,395,523 + -1.9 1,395,523 + -5.0 1,395,523 + -1.6 1,395,528 + +0.5 1,395,554 - +0.8 1,395,555 + +0.6 1,395,558 - +0.8 1,395,558 - -0.1 1,395,559 + +0.5 1,395,560 - +0.5 1,395,561 + +1.0 1,395,561 + +0.3 1,395,561 + +0.3 1,395,561 + +0.0 1,395,563 + +1.4 1,395,564 - -1.7 1,395,564 - -0.2 1,395,564 - -1.0 1,395,565 + +0.8 1,395,565 + -0.1 1,395,565 + +1.0 1,395,565 + +0.6 1,395,758 - -0.3 1,395,758 - -1.4 1,395,787 + +0.3 1,395,787 + +0.3 1,395,960 + MMP_RS07330 0.14 -0.2 1,396,053 + MMP_RS07330 0.17 +0.6 1,396,106 - MMP_RS07330 0.20 -0.9 1,396,106 - MMP_RS07330 0.20 -3.9 1,396,107 + MMP_RS07330 0.20 -1.1 1,396,107 + MMP_RS07330 0.20 -0.1 1,396,157 + MMP_RS07330 0.22 -1.4 1,396,158 - MMP_RS07330 0.22 -1.5 1,396,158 - MMP_RS07330 0.22 -0.4 1,396,159 + MMP_RS07330 0.22 -0.6 1,396,159 + MMP_RS07330 0.22 -0.8 1,396,160 - MMP_RS07330 0.22 -1.1 1,396,201 - MMP_RS07330 0.23 -0.3 1,396,213 - MMP_RS07330 0.24 -1.1 1,396,222 - MMP_RS07330 0.24 -0.3 1,396,311 - MMP_RS07330 0.28 -0.2 1,396,370 + MMP_RS07330 0.30 -1.0 1,396,372 - MMP_RS07330 0.30 +0.6 1,396,372 - MMP_RS07330 0.30 +0.6 1,396,373 + MMP_RS07330 0.30 -0.2 1,396,374 - MMP_RS07330 0.30 +0.5 1,396,374 - MMP_RS07330 0.30 +0.2 1,396,383 - MMP_RS07330 0.30 -0.2 1,396,383 - MMP_RS07330 0.30 +0.1 1,396,384 + MMP_RS07330 0.30 -0.9 1,396,394 + MMP_RS07330 0.31 +0.3 1,396,394 + MMP_RS07330 0.31 -0.6 1,396,394 + MMP_RS07330 0.31 -1.1 1,396,439 - MMP_RS07330 0.33 +0.5 1,396,440 + MMP_RS07330 0.33 -1.6 1,396,440 + MMP_RS07330 0.33 -0.7 1,396,445 + MMP_RS07330 0.33 -1.7 1,396,445 + MMP_RS07330 0.33 -0.4 1,396,445 + MMP_RS07330 0.33 -0.3 1,396,445 + MMP_RS07330 0.33 -0.3 1,396,445 + MMP_RS07330 0.33 -1.4 1,396,494 + MMP_RS07330 0.35 -1.3 1,396,496 - MMP_RS07330 0.35 +0.5
Or see this region's nucleotide sequence