Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07235 and MMP_RS07240 are separated by 69 nucleotides MMP_RS07240 and MMP_RS07245 are separated by 165 nucleotides MMP_RS07245 and MMP_RS07250 are separated by 20 nucleotides MMP_RS07250 and MMP_RS07255 are separated by 19 nucleotides MMP_RS07255 and MMP_RS07260 are separated by 11 nucleotides
MMP_RS07235: MMP_RS07235 - stress response translation initiation inhibitor YciH, at 1,385,447 to 1,385,755
_RS07235
MMP_RS07240: MMP_RS07240 - ribonuclease P protein component 1, at 1,385,825 to 1,386,121
_RS07240
MMP_RS07245: MMP_RS07245 - 30S ribosomal protein S17, at 1,386,287 to 1,386,616
_RS07245
MMP_RS07250: MMP_RS07250 - 50S ribosomal protein L14, at 1,386,637 to 1,387,035
_RS07250
MMP_RS07255: MMP_RS07255 - 50S ribosomal protein L24, at 1,387,055 to 1,387,414
_RS07255
MMP_RS07260: MMP_RS07260 - 30S ribosomal protein S4e, at 1,387,426 to 1,388,160
_RS07260
Position (kb) 1386
1387
1388 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1385.743 kb on + strand at 1385.743 kb on + strand at 1386.111 kb on + strand at 1386.195 kb on - strand at 1386.211 kb on + strand at 1386.287 kb on + strand at 1386.453 kb on - strand at 1386.637 kb on + strand at 1386.750 kb on + strand at 1386.900 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,385,743 + -4.5 1,385,743 + -0.4 1,386,111 + -4.1 1,386,195 - -1.0 1,386,211 + -2.0 1,386,287 + -1.6 1,386,453 - -3.0 1,386,637 + -2.6 1,386,750 + -1.2 1,386,900 + -3.3
Or see this region's nucleotide sequence