Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07210 and MMP_RS07215 are separated by 142 nucleotides MMP_RS07215 and MMP_RS07220 are separated by 19 nucleotides MMP_RS07220 and MMP_RS07225 are separated by 10 nucleotides MMP_RS07225 and MMP_RS07230 are separated by 10 nucleotides MMP_RS07230 and MMP_RS07235 are separated by 83 nucleotides MMP_RS07235 and MMP_RS07240 are separated by 69 nucleotides
MMP_RS07210: MMP_RS07210 - elongation factor 1-beta, at 1,383,440 to 1,383,709
_RS07210
MMP_RS07215: MMP_RS07215 - hypothetical protein, at 1,383,852 to 1,384,010
_RS07215
MMP_RS07220: MMP_RS07220 - 50S ribosomal protein L22, at 1,384,030 to 1,384,491
_RS07220
MMP_RS07225: MMP_RS07225 - 30S ribosomal protein S3, at 1,384,502 to 1,385,137
_RS07225
MMP_RS07230: MMP_RS07230 - 50S ribosomal protein L29, at 1,385,148 to 1,385,363
_RS07230
MMP_RS07235: MMP_RS07235 - stress response translation initiation inhibitor YciH, at 1,385,447 to 1,385,755
_RS07235
MMP_RS07240: MMP_RS07240 - ribonuclease P protein component 1, at 1,385,825 to 1,386,121
_RS07240
Position (kb) 1384
1385
1386 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1383.596 kb on - strand at 1383.597 kb on + strand at 1385.368 kb on + strand at 1385.406 kb on + strand at 1385.743 kb on + strand at 1385.743 kb on + strand at 1386.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,383,596 - -3.8 1,383,597 + -4.6 1,385,368 + -0.2 1,385,406 + -2.8 1,385,743 + -4.5 1,385,743 + -0.4 1,386,111 + -4.1
Or see this region's nucleotide sequence