Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07160 and MMP_RS07165 are separated by 78 nucleotides MMP_RS07165 and MMP_RS07170 are separated by 138 nucleotides MMP_RS07170 and MMP_RS07175 are separated by 2 nucleotides
MMP_RS07160: MMP_RS07160 - aspartate-semialdehyde dehydrogenase, at 1,369,848 to 1,370,894
_RS07160
MMP_RS07165: MMP_RS07165 - RNA-splicing ligase RtcB, at 1,370,973 to 1,372,415
_RS07165
MMP_RS07170: MMP_RS07170 - DUF371 domain-containing protein, at 1,372,554 to 1,372,970
_RS07170
MMP_RS07175: MMP_RS07175 - type I 3-dehydroquinate dehydratase, at 1,372,973 to 1,373,629
_RS07175
Position (kb)
1370
1371
1372
1373 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1370.154 kb on + strand at 1371.067 kb on - strand at 1371.450 kb on - strand at 1371.632 kb on - strand at 1371.632 kb on - strand at 1371.673 kb on - strand at 1371.791 kb on + strand at 1372.079 kb on - strand at 1372.450 kb on - strand at 1372.457 kb on - strand at 1372.463 kb on + strand at 1372.463 kb on + strand at 1372.464 kb on - strand at 1372.494 kb on - strand at 1372.516 kb on - strand at 1372.958 kb on + strand at 1373.241 kb on + strand at 1373.327 kb on + strand at 1373.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,370,154 + -4.9 1,371,067 - -2.1 1,371,450 - -0.8 1,371,632 - -2.6 1,371,632 - -4.7 1,371,673 - -0.4 1,371,791 + -2.7 1,372,079 - -1.5 1,372,450 - -0.2 1,372,457 - -0.0 1,372,463 + -0.0 1,372,463 + -1.7 1,372,464 - -1.2 1,372,494 - -1.7 1,372,516 - +0.8 1,372,958 + +0.0 1,373,241 + -3.0 1,373,327 + -3.6 1,373,360 - -2.8
Or see this region's nucleotide sequence