Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07035 and MMP_RS07040 are separated by 197 nucleotides MMP_RS07040 and MMP_RS07045 are separated by 16 nucleotides MMP_RS07045 and MMP_RS07050 are separated by 9 nucleotides MMP_RS07050 and MMP_RS07055 are separated by 33 nucleotides
MMP_RS07035: MMP_RS07035 - DNA-directed RNA polymerase subunit A'', at 1,345,497 to 1,346,657
_RS07035
MMP_RS07040: MMP_RS07040 - 50S ribosomal protein L30e, at 1,346,855 to 1,347,175
_RS07040
MMP_RS07045: MMP_RS07045 - NusA-like transcription termination signal-binding factor, at 1,347,192 to 1,347,713
_RS07045
MMP_RS07050: MMP_RS07050 - 30S ribosomal protein S12, at 1,347,723 to 1,348,166
_RS07050
MMP_RS07055: MMP_RS07055 - 30S ribosomal protein S7, at 1,348,200 to 1,348,766
_RS07055
Position (kb)
1347
1348 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1346.209 kb on + strand at 1346.617 kb on - strand at 1346.617 kb on - strand at 1346.617 kb on - strand at 1346.618 kb on + strand at 1346.618 kb on + strand at 1346.698 kb on - strand at 1346.708 kb on + strand at 1346.739 kb on - strand at 1346.740 kb on + strand at 1346.880 kb on - strand at 1346.899 kb on + strand at 1348.302 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,346,209 + -3.2 1,346,617 - -4.0 1,346,617 - -4.1 1,346,617 - -4.7 1,346,618 + -2.3 1,346,618 + -4.1 1,346,698 - +0.7 1,346,708 + +0.4 1,346,739 - +0.3 1,346,740 + +0.7 1,346,880 - -2.3 1,346,899 + -0.8 1,348,302 + -2.3
Or see this region's nucleotide sequence