Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07030 and MMP_RS07035 are separated by 44 nucleotides MMP_RS07035 and MMP_RS07040 are separated by 197 nucleotides MMP_RS07040 and MMP_RS07045 are separated by 16 nucleotides
MMP_RS07030: MMP_RS07030 - DNA-directed RNA polymerase subunit A', at 1,342,783 to 1,345,452
_RS07030
MMP_RS07035: MMP_RS07035 - DNA-directed RNA polymerase subunit A'', at 1,345,497 to 1,346,657
_RS07035
MMP_RS07040: MMP_RS07040 - 50S ribosomal protein L30e, at 1,346,855 to 1,347,175
_RS07040
MMP_RS07045: MMP_RS07045 - NusA-like transcription termination signal-binding factor, at 1,347,192 to 1,347,713
_RS07045
Position (kb)
1345
1346
1347 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1344.563 kb on + strand at 1344.639 kb on + strand at 1344.639 kb on + strand at 1344.684 kb on + strand at 1344.829 kb on + strand at 1344.958 kb on - strand at 1344.963 kb on + strand at 1345.307 kb on + strand at 1345.479 kb on - strand at 1346.209 kb on + strand at 1346.617 kb on - strand at 1346.617 kb on - strand at 1346.617 kb on - strand at 1346.618 kb on + strand at 1346.618 kb on + strand at 1346.698 kb on - strand at 1346.708 kb on + strand at 1346.739 kb on - strand at 1346.740 kb on + strand at 1346.880 kb on - strand at 1346.899 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,344,563 + -4.0 1,344,639 + -1.3 1,344,639 + -1.4 1,344,684 + -2.0 1,344,829 + -2.0 1,344,958 - -1.2 1,344,963 + -1.6 1,345,307 + -3.8 1,345,479 - -2.0 1,346,209 + -3.2 1,346,617 - -4.0 1,346,617 - -4.1 1,346,617 - -4.7 1,346,618 + -2.3 1,346,618 + -4.1 1,346,698 - +0.7 1,346,708 + +0.4 1,346,739 - +0.3 1,346,740 + +0.7 1,346,880 - -2.3 1,346,899 + -0.8
Or see this region's nucleotide sequence