Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06995 and MMP_RS07000 are separated by 15 nucleotides MMP_RS07000 and MMP_RS07005 are separated by 44 nucleotides MMP_RS07005 and MMP_RS07010 are separated by 13 nucleotides MMP_RS07010 and MMP_RS07015 are separated by 253 nucleotides MMP_RS07015 and MMP_RS07020 are separated by 81 nucleotides
MMP_RS06995: MMP_RS06995 - TIGR00269 family protein, at 1,335,578 to 1,336,513
_RS06995
MMP_RS07000: MMP_RS07000 - MoaD/ThiS family protein, at 1,336,529 to 1,336,783
_RS07000
MMP_RS07005: MMP_RS07005 - 4Fe-4S binding protein, at 1,336,828 to 1,337,226
_RS07005
MMP_RS07010: MMP_RS07010 - homocysteine biosynthesis protein, at 1,337,240 to 1,338,781
_RS07010
MMP_RS07015: MMP_RS07015 - DNA-directed RNA polymerase subunit H, at 1,339,035 to 1,339,271
_RS07015
MMP_RS07020: MMP_RS07020 - DNA-directed RNA polymerase subunit B'', at 1,339,353 to 1,340,852
_RS07020
Position (kb)
1337
1338
1339 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1336.447 kb on - strand at 1336.448 kb on + strand at 1336.480 kb on + strand at 1336.481 kb on - strand at 1336.517 kb on + strand at 1336.551 kb on - strand at 1336.553 kb on - strand at 1336.553 kb on - strand at 1336.580 kb on - strand at 1336.581 kb on + strand at 1336.622 kb on - strand at 1336.685 kb on - strand at 1336.827 kb on + strand at 1337.128 kb on - strand, within MMP_RS07005 at 1337.405 kb on - strand at 1337.406 kb on + strand at 1337.407 kb on - strand at 1337.407 kb on - strand at 1337.407 kb on - strand at 1337.408 kb on + strand at 1337.409 kb on - strand at 1337.409 kb on - strand at 1337.410 kb on + strand at 1337.410 kb on + strand at 1337.414 kb on + strand at 1337.683 kb on - strand at 1337.835 kb on - strand at 1337.836 kb on + strand at 1338.159 kb on - strand at 1338.159 kb on - strand at 1338.201 kb on + strand at 1338.259 kb on + strand at 1338.259 kb on + strand at 1338.259 kb on + strand at 1338.300 kb on - strand at 1338.398 kb on + strand at 1338.402 kb on - strand at 1338.804 kb on + strand at 1338.822 kb on + strand at 1338.827 kb on + strand at 1338.857 kb on - strand at 1338.858 kb on + strand at 1338.859 kb on - strand at 1338.862 kb on - strand at 1338.864 kb on - strand at 1338.864 kb on - strand at 1338.864 kb on - strand at 1338.864 kb on - strand at 1338.865 kb on + strand at 1338.866 kb on - strand at 1338.867 kb on + strand at 1338.867 kb on + strand at 1338.873 kb on - strand at 1338.878 kb on - strand at 1338.879 kb on + strand at 1338.880 kb on - strand at 1338.880 kb on - strand at 1338.882 kb on - strand at 1338.883 kb on + strand at 1338.883 kb on + strand at 1338.889 kb on + strand at 1338.926 kb on + strand at 1338.942 kb on - strand at 1338.945 kb on - strand at 1338.948 kb on + strand at 1339.284 kb on + strand at 1339.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,336,447 - -0.2 1,336,448 + -3.2 1,336,480 + -1.0 1,336,481 - -2.9 1,336,517 + -1.4 1,336,551 - -2.2 1,336,553 - -0.7 1,336,553 - +0.3 1,336,580 - -0.0 1,336,581 + -1.3 1,336,622 - +1.0 1,336,685 - -2.5 1,336,827 + -1.6 1,337,128 - MMP_RS07005 0.75 -1.5 1,337,405 - -2.9 1,337,406 + -0.7 1,337,407 - -1.5 1,337,407 - -4.5 1,337,407 - -3.8 1,337,408 + -2.0 1,337,409 - -1.0 1,337,409 - -3.3 1,337,410 + -3.2 1,337,410 + -3.2 1,337,414 + -3.5 1,337,683 - -5.0 1,337,835 - -3.0 1,337,836 + -2.4 1,338,159 - -2.3 1,338,159 - -2.7 1,338,201 + -3.0 1,338,259 + -1.0 1,338,259 + -4.4 1,338,259 + -1.1 1,338,300 - -1.2 1,338,398 + -4.0 1,338,402 - -2.8 1,338,804 + -3.6 1,338,822 + -2.6 1,338,827 + -3.5 1,338,857 - +0.3 1,338,858 + -1.7 1,338,859 - -1.0 1,338,862 - +0.3 1,338,864 - -0.5 1,338,864 - -0.7 1,338,864 - -1.2 1,338,864 - +1.8 1,338,865 + -3.7 1,338,866 - -2.3 1,338,867 + -2.8 1,338,867 + -1.9 1,338,873 - +0.6 1,338,878 - +0.0 1,338,879 + -1.4 1,338,880 - +0.5 1,338,880 - +0.1 1,338,882 - +0.2 1,338,883 + +0.1 1,338,883 + +0.6 1,338,889 + +0.4 1,338,926 + +1.9 1,338,942 - -1.9 1,338,945 - +0.7 1,338,948 + -0.7 1,339,284 + -1.5 1,339,317 - -1.5
Or see this region's nucleotide sequence