Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08915 and MMP_RS06960 are separated by 27 nucleotides MMP_RS06960 and MMP_RS06965 are separated by 16 nucleotides
MMP_RS08915: MMP_RS08915 - 2 5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,327,842 to 1,328,924
_RS08915
MMP_RS06960: MMP_RS06960 - NAD+ synthase, at 1,328,952 to 1,329,728
_RS06960
MMP_RS06965: MMP_RS06965 - TIGR04014 family B12-binding domain/radical SAM domain-containing protein, at 1,329,745 to 1,331,058
_RS06965
Position (kb)
1328
1329
1330 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1327.961 kb on + strand, within MMP_RS08915 at 1328.272 kb on + strand, within MMP_RS08915 at 1328.499 kb on + strand, within MMP_RS08915 at 1328.524 kb on + strand, within MMP_RS08915 at 1328.627 kb on - strand, within MMP_RS08915 at 1328.664 kb on - strand, within MMP_RS08915 at 1328.677 kb on - strand, within MMP_RS08915 at 1329.051 kb on + strand at 1329.290 kb on + strand at 1329.347 kb on + strand at 1329.349 kb on - strand at 1329.352 kb on + strand at 1329.546 kb on - strand at 1329.735 kb on + strand at 1329.768 kb on - strand at 1329.768 kb on - strand at 1329.768 kb on - strand at 1329.796 kb on + strand at 1329.849 kb on - strand at 1329.923 kb on + strand, within MMP_RS06965 at 1329.930 kb on + strand, within MMP_RS06965 at 1330.037 kb on + strand, within MMP_RS06965 at 1330.068 kb on - strand, within MMP_RS06965 at 1330.283 kb on + strand, within MMP_RS06965 at 1330.346 kb on + strand, within MMP_RS06965 at 1330.348 kb on + strand, within MMP_RS06965
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,327,961 + MMP_RS08915 0.11 -3.3 1,328,272 + MMP_RS08915 0.40 -2.7 1,328,499 + MMP_RS08915 0.61 -2.3 1,328,524 + MMP_RS08915 0.63 +0.6 1,328,627 - MMP_RS08915 0.72 -0.9 1,328,664 - MMP_RS08915 0.76 +0.3 1,328,677 - MMP_RS08915 0.77 +0.4 1,329,051 + -1.5 1,329,290 + -2.4 1,329,347 + -2.8 1,329,349 - -3.9 1,329,352 + -2.7 1,329,546 - -4.2 1,329,735 + -4.0 1,329,768 - -0.3 1,329,768 - -0.7 1,329,768 - -1.8 1,329,796 + +0.4 1,329,849 - +0.4 1,329,923 + MMP_RS06965 0.14 +0.3 1,329,930 + MMP_RS06965 0.14 -0.1 1,330,037 + MMP_RS06965 0.22 -0.4 1,330,068 - MMP_RS06965 0.25 +1.0 1,330,283 + MMP_RS06965 0.41 +0.7 1,330,346 + MMP_RS06965 0.46 -0.0 1,330,348 + MMP_RS06965 0.46 +0.1
Or see this region's nucleotide sequence