Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06940 and MMP_RS06945 are separated by 16 nucleotides MMP_RS06945 and MMP_RS06950 are separated by 129 nucleotides MMP_RS06950 and MMP_RS08915 are separated by 189 nucleotides
MMP_RS06940: MMP_RS06940 - polyprenyl diphosphate synthase, at 1,325,780 to 1,326,574
_RS06940
MMP_RS06945: MMP_RS06945 - methanogenesis marker protein Mmp4/MtxX, at 1,326,591 to 1,327,322
_RS06945
MMP_RS06950: MMP_RS06950 - histone family protein, at 1,327,452 to 1,327,652
_RS06950
MMP_RS08915: MMP_RS08915 - 2 5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase, at 1,327,842 to 1,328,924
_RS08915
Position (kb)
1327
1328 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1326.657 kb on + strand at 1326.660 kb on - strand at 1326.663 kb on + strand at 1326.734 kb on - strand at 1326.742 kb on + strand at 1326.774 kb on - strand at 1326.797 kb on + strand at 1326.797 kb on + strand at 1326.807 kb on - strand at 1326.999 kb on - strand at 1326.999 kb on - strand at 1327.023 kb on - strand at 1327.104 kb on + strand at 1327.318 kb on - strand at 1327.318 kb on - strand at 1327.319 kb on + strand at 1327.334 kb on - strand at 1327.346 kb on + strand at 1327.374 kb on + strand at 1327.376 kb on - strand at 1327.384 kb on - strand at 1327.384 kb on - strand at 1327.384 kb on - strand at 1327.384 kb on - strand at 1327.385 kb on + strand at 1327.385 kb on + strand at 1327.386 kb on - strand at 1327.387 kb on + strand at 1327.404 kb on - strand at 1327.405 kb on + strand at 1327.419 kb on - strand at 1327.422 kb on - strand at 1327.742 kb on - strand at 1327.816 kb on - strand at 1327.844 kb on + strand at 1327.961 kb on + strand, within MMP_RS08915 at 1328.272 kb on + strand, within MMP_RS08915 at 1328.499 kb on + strand, within MMP_RS08915 at 1328.524 kb on + strand, within MMP_RS08915 at 1328.627 kb on - strand, within MMP_RS08915
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,326,657 + -1.6 1,326,660 - -2.5 1,326,663 + -3.5 1,326,734 - -2.6 1,326,742 + -2.1 1,326,774 - -3.5 1,326,797 + -0.3 1,326,797 + -2.6 1,326,807 - -2.1 1,326,999 - +0.2 1,326,999 - -2.6 1,327,023 - -3.1 1,327,104 + -3.2 1,327,318 - -2.3 1,327,318 - -4.4 1,327,319 + -3.9 1,327,334 - -0.3 1,327,346 + +0.1 1,327,374 + -1.5 1,327,376 - +1.2 1,327,384 - -1.9 1,327,384 - +1.1 1,327,384 - +0.4 1,327,384 - -2.0 1,327,385 + -2.9 1,327,385 + -0.8 1,327,386 - +0.7 1,327,387 + +0.2 1,327,404 - +0.5 1,327,405 + +0.1 1,327,419 - -0.5 1,327,422 - +0.5 1,327,742 - -1.6 1,327,816 - +0.3 1,327,844 + +0.4 1,327,961 + MMP_RS08915 0.11 -3.3 1,328,272 + MMP_RS08915 0.40 -2.7 1,328,499 + MMP_RS08915 0.61 -2.3 1,328,524 + MMP_RS08915 0.63 +0.6 1,328,627 - MMP_RS08915 0.72 -0.9
Or see this region's nucleotide sequence