Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06820 and MMP_RS06825 are separated by 56 nucleotides MMP_RS06825 and MMP_RS06830 are separated by 20 nucleotides MMP_RS06830 and MMP_RS06835 are separated by 13 nucleotides MMP_RS06835 and MMP_RS06840 are separated by 38 nucleotides MMP_RS06840 and MMP_RS06845 are separated by 37 nucleotides MMP_RS06845 and MMP_RS06850 are separated by 37 nucleotides
MMP_RS06820: MMP_RS06820 - DNA-directed RNA polymerase subunit D, at 1,307,356 to 1,307,898
_RS06820
MMP_RS06825: MMP_RS06825 - 50S ribosomal protein L18e, at 1,307,955 to 1,308,317
_RS06825
MMP_RS06830: MMP_RS06830 - 50S ribosomal protein L13, at 1,308,338 to 1,308,751
_RS06830
MMP_RS06835: MMP_RS06835 - 30S ribosomal protein S9, at 1,308,765 to 1,309,169
_RS06835
MMP_RS06840: MMP_RS06840 - DNA-directed RNA polymerase subunit N, at 1,309,208 to 1,309,414
_RS06840
MMP_RS06845: MMP_RS06845 - tRNA-Pro, at 1,309,452 to 1,309,525
_RS06845
MMP_RS06850: MMP_RS06850 - DNA-directed RNA polymerase subunit K, at 1,309,563 to 1,309,736
_RS06850
Position (kb)
1308
1309 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1307.432 kb on + strand at 1309.168 kb on + strand at 1309.387 kb on + strand, within MMP_RS06840 at 1309.407 kb on + strand at 1309.425 kb on + strand at 1309.432 kb on - strand at 1309.436 kb on + strand at 1309.540 kb on + strand at 1309.672 kb on - strand at 1309.742 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,307,432 + -3.8 1,309,168 + -1.7 1,309,387 + MMP_RS06840 0.86 -1.5 1,309,407 + -2.5 1,309,425 + -1.5 1,309,432 - -2.3 1,309,436 + -1.0 1,309,540 + -0.6 1,309,672 - -2.8 1,309,742 - -1.0
Or see this region's nucleotide sequence