Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06790 and MMP_RS06795 are separated by 203 nucleotides MMP_RS06795 and MMP_RS06800 are separated by 401 nucleotides MMP_RS06800 and MMP_RS06805 are separated by 58 nucleotides MMP_RS06805 and MMP_RS06810 are separated by 23 nucleotides MMP_RS06810 and MMP_RS06815 are separated by 9 nucleotides
MMP_RS06790: MMP_RS06790 - lysine--tRNA ligase, at 1,303,479 to 1,305,080
_RS06790
MMP_RS06795: MMP_RS06795 - tRNA-Cys, at 1,305,284 to 1,305,357
_RS06795
MMP_RS06800: MMP_RS06800 - tRNA-Ser, at 1,305,759 to 1,305,846
_RS06800
MMP_RS06805: MMP_RS06805 - 30S ribosomal protein S13, at 1,305,905 to 1,306,354
_RS06805
MMP_RS06810: MMP_RS06810 - 30S ribosomal protein S4, at 1,306,378 to 1,306,914
_RS06810
MMP_RS06815: MMP_RS06815 - 30S ribosomal protein S11, at 1,306,924 to 1,307,298
_RS06815
Position (kb)
1305
1306
1307 Strain fitness (log2 ratio)
-2
-1
0
1 at 1305.195 kb on - strand at 1305.196 kb on + strand at 1305.211 kb on - strand at 1305.463 kb on - strand at 1305.488 kb on - strand at 1305.493 kb on - strand at 1305.495 kb on - strand at 1305.495 kb on - strand at 1305.496 kb on + strand at 1305.515 kb on - strand at 1305.515 kb on - strand at 1305.516 kb on + strand at 1305.516 kb on + strand at 1305.516 kb on + strand at 1305.517 kb on - strand at 1305.544 kb on - strand at 1305.562 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,305,195 - +0.1 1,305,196 + -1.5 1,305,211 - -0.2 1,305,463 - +0.2 1,305,488 - +0.7 1,305,493 - +0.3 1,305,495 - -0.7 1,305,495 - +0.3 1,305,496 + -0.3 1,305,515 - +0.7 1,305,515 - -1.1 1,305,516 + -2.7 1,305,516 + -0.8 1,305,516 + +0.5 1,305,517 - +1.5 1,305,544 - -0.9 1,305,562 + +0.2
Or see this region's nucleotide sequence