Experiment: H2 N-free with 10mM L-Aspartic Acid
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06770 and MMP_RS06775 are separated by 320 nucleotides MMP_RS06775 and MMP_RS06780 are separated by 9 nucleotides MMP_RS06780 and MMP_RS06785 are separated by 99 nucleotides
MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796
_RS06770
MMP_RS06775: MMP_RS06775 - 2-oxoacid:ferredoxin oxidoreductase subunit gamma, at 1,301,117 to 1,301,650
_RS06775
MMP_RS06780: MMP_RS06780 - 2-oxoacid:ferredoxin oxidoreductase subunit beta, at 1,301,660 to 1,302,457
_RS06780
MMP_RS06785: MMP_RS06785 - hypothetical protein, at 1,302,557 to 1,303,333
_RS06785
Position (kb)
1301
1302
1303 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1300.797 kb on + strand at 1300.799 kb on - strand at 1300.836 kb on + strand at 1300.878 kb on - strand at 1300.879 kb on + strand at 1300.881 kb on + strand at 1300.882 kb on - strand at 1300.884 kb on - strand at 1300.884 kb on - strand at 1300.958 kb on + strand at 1300.960 kb on + strand at 1301.108 kb on - strand at 1301.444 kb on + strand at 1301.588 kb on - strand at 1303.135 kb on - strand at 1303.321 kb on - strand at 1303.363 kb on - strand at 1303.366 kb on + strand at 1303.369 kb on + strand at 1303.376 kb on - strand at 1303.406 kb on - strand at 1303.440 kb on - strand at 1303.441 kb on + strand at 1303.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Aspartic Acid remove 1,300,797 + +0.1 1,300,799 - -0.1 1,300,836 + -1.1 1,300,878 - -0.0 1,300,879 + -0.2 1,300,881 + +0.1 1,300,882 - +0.7 1,300,884 - +0.2 1,300,884 - +1.5 1,300,958 + -2.5 1,300,960 + +0.3 1,301,108 - +0.3 1,301,444 + -0.8 1,301,588 - -0.6 1,303,135 - -1.5 1,303,321 - -3.0 1,303,363 - -1.4 1,303,366 + +1.0 1,303,369 + -2.8 1,303,376 - +0.1 1,303,406 - +0.2 1,303,440 - -0.5 1,303,441 + -1.7 1,303,444 - +0.1
Or see this region's nucleotide sequence